{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T03:39:38Z","timestamp":1773286778424,"version":"3.50.1"},"reference-count":32,"publisher":"American Veterinary Medical Association (AVMA)","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To investigate the influence of oxidative stress in terms of antioxidant capacity and lipid peroxidation on the probability of motor neuron disease (MND) in horses.<\/jats:p>\n<jats:p><jats:bold>Animals<\/jats:bold>\u201488 horses with MND (cases) and 49 controls.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014Blood samples were collected from all horses enrolled, and RBCs and plasma were harvested. Activity of the enzyme erythrocytic superoxide dismutase 1 (SOD1) was determined in the RBCs. Plasma concentrations of \u03b1-tocopherols and \u03b2-carotenes and activity of glutathione peroxidase were also evaluated. Degree of lipid peroxidation was measured by determining plasma concentrations of lipid hydroperoxides. Differences were evaluated between horse groups.<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014Cases had lower erythrocyte SOD1 activity than did controls, but the difference was not significant. On the other hand, plasma vitamin E concentrations differed significantly between groups, with the cases having lower concentrations. Neither plasma vitamin A concentration nor glutathione peroxidase activity differed between groups; however, cases had significantly higher concentrations of lipid hydroperoxides (18.53\u03bcM) than did controls (12.35\u03bcM).<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Horses with MND differed from those without MND by having a lower plasma concentration of vitamin E and higher concentrations of lipid hydroperoxides. Results parallel the findings in humans with sporadic amyotrophic sclerosis and provide evidence supporting the involvement of oxidative stress in the 2 conditions.<\/jats:p>","DOI":"10.2460\/ajvr.73.12.1957","type":"journal-article","created":{"date-parts":[[2012,11,23]],"date-time":"2012-11-23T17:26:48Z","timestamp":1353691608000},"page":"1957-1962","source":"Crossref","is-referenced-by-count":14,"title":["Association of oxidative stress with motor neuron disease in horses"],"prefix":"10.2460","volume":"73","author":[{"given":"Hussni O.","family":"Mohammed","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas J.","family":"Divers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justin","family":"Kwak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adil 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Res","doi-asserted-by":"publisher","first-page":"120","DOI":"10.2460\/ajvr.67.1.120","article-title":"Evaluation of the risk of motor neuron disease in horses fed a diet low in vitamin E and high in copper and iron","volume":"67","author":"Divers","year":"2006"},{"key":"ref4","series-title":"Acta Neuropathol (Berl)","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1007\/BF00227725","article-title":"Eosinophilic cytoplasmic inclusions in sporadic equine motor neuron disease: an electron microscopic study","volume":"85","author":"Cummings","year":"1993"},{"key":"ref5","series-title":"Acta Vet Scand","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1186\/1751-0147-49-17","article-title":"Vitamin E deficiency and risk of equine motor neuron disease","volume":"49","author":"Mohammed","year":"2007"},{"key":"ref6","series-title":"J Am Vet Med Assoc","first-page":"1261","article-title":"Intrinsic, management, and nutritional factors associated with equine motor neuron disease","volume":"211","author":"Rua-Domenech","year":"1997"},{"key":"ref7","series-title":"Equine Vet J","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1111\/j.2042-3306.1999.tb03801.x","article-title":"Ocular manifestations of equine motor neuron disease","volume":"31","author":"Riis","year":"1999"},{"key":"ref8","series-title":"Antioxid Redox Signal","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1089\/ars.2009.2668","article-title":"Lipid oxidation and peroxidation in CNS health and disease: from molecular mechanisms to therapeutic opportunities","volume":"12","author":"Adibhatla","year":"2010"},{"key":"ref9","series-title":"Neurochem Res","doi-asserted-by":"publisher","first-page":"1530","DOI":"10.1007\/s11064-010-0212-5","article-title":"The role of oxidative stress in amyotrophic lateral sclerosis and Parkinson's disease","volume":"35","author":"Baillet","year":"2010"},{"key":"ref10","series-title":"J Neurochem","doi-asserted-by":"publisher","first-page":"2322","DOI":"10.1111\/j.1471-4159.1993.tb07478.x","article-title":"Superoxide dismutase activity, oxidative damage, and mitochondrial energy metabolism in familial and sporadic amyotrophic lateral sclerosis","volume":"61","author":"Bowling","year":"1993"},{"key":"ref11","series-title":"Gene","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/0378-1119(96)00339-3","article-title":"Equine motor neuron disease is not linked to Cu\/Zn superoxide dismutase mutations: sequence analysis of the equine Cu\/Zn superoxide dismutase cDNA","volume":"178","author":"Rua-Domenech","year":"1996"},{"key":"ref12","series-title":"Equine Vet J","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1111\/j.2042-3306.1994.tb04411.x","article-title":"Equine motor neuron disease: findings in 28 horses and proposal of a pathophysiological mechanism for the disease","volume":"26","author":"Divers","year":"1994"},{"key":"ref13","series-title":"Cancer Res","first-page":"6674","article-title":"Changes in CuZn-superoxide dismutase during induced differentiation of murine erythroleukemia cells","volume":"48","author":"Paoletti","year":"1988"},{"key":"ref14","series-title":"J Lab Clin Med","first-page":"158","article-title":"Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase","volume":"70","author":"Paglia","year":"1967"},{"key":"ref15","series-title":"Free Radic Biol Med","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1016\/j.freeradbiomed.2009.11.018","article-title":"Oxidative stress in ALS: key role in motor neuron injury and therapeutic target","volume":"48","author":"Barber","year":"2010"},{"key":"ref16","series-title":"Neurol Res","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1179\/016164105X18430","article-title":"Oxidative stress and metal content in blood and cerebrospinal fluid of amyotrophic lateral sclerosis patients with and without a Cu, Zn-superoxide dismutase mutation","volume":"27","author":"Ihara","year":"2005"},{"key":"ref17","series-title":"Nutr Neurosci","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1080\/10284150290028954","article-title":"Vitamin E and neurodegenerative disorders associated with oxidative stress","volume":"5","author":"Butterfield","year":"2002"},{"key":"ref18","series-title":"J Clin Neurosci","doi-asserted-by":"publisher","first-page":"991","DOI":"10.1016\/j.jocn.2005.10.019","article-title":"Red cell superoxide dismutase activity in sporadic amyotrophic lateral sclerosis","volume":"13","author":"Tuncel","year":"2006"},{"issue":"suppl","key":"ref19","series-title":"J Neurol Sci","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/0022-510X(95)00074-C","article-title":"Cu, Zn SOD in German families with ALS","volume":"129","author":"Bachus","year":"1995"},{"issue":"suppl 1","key":"ref20","series-title":"Ann Neurol","doi-asserted-by":"publisher","first-page":"S17","DOI":"10.1002\/ana.21544","article-title":"Managing amyotrophic lateral sclerosis: slowing disease progression and improving patient quality of life","volume":"65","author":"Brooks","year":"2009"},{"key":"ref21","series-title":"Neuroepidemiology","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1159\/000201563","article-title":"Fruit and vegetable intake and risk of amyotrophic lateral sclerosis in Japan","volume":"32","author":"Okamoto","year":"2009"},{"key":"ref22","series-title":"Am J Epidemiol","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1093\/aje\/kwq416","article-title":"Vitamin E intake and risk of amyotrophic lateral sclerosis: a pooled analysis of data from 5 prospective cohort studies","volume":"173","author":"Wang","year":"2011"},{"key":"ref23","series-title":"J Neural Transm","doi-asserted-by":"publisher","first-page":"1021","DOI":"10.1007\/s007020070050","article-title":"Serum levels of coenzyme Q10 in patients with amyotrophic lateral sclerosis","volume":"107","author":"Molina","year":"2000"},{"key":"ref24","series-title":"Acta Neuropathol","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1007\/s00401-005-1019-3","article-title":"Redox system expression in the motor neurons in amyotrophic lateral sclerosis (ALS): immunohistochemical studies on sporadic ALS, superoxide dismutase 1 (SOD1)-mutated familial ALS, and SOD1-mutated ALS animal models","volume":"110","author":"Kato","year":"2005"},{"key":"ref25","series-title":"J Neurol","doi-asserted-by":"publisher","first-page":"762","DOI":"10.1007\/s00415-010-5825-8","article-title":"Monitoring systemic oxidative stress in an animal model of amyotrophic lateral sclerosis","volume":"258","author":"Miana-Mena","year":"2011"},{"key":"ref26","series-title":"J Neurol Sci","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/S0022-510X(00)00365-8","article-title":"Blood oxidative stress in amyotrophic lateral sclerosis","volume":"178","author":"Bonnefont-Rousselot","year":"2000"},{"key":"ref27","series-title":"Neurochem Res","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1023\/A:1027384432715","article-title":"Evaluation of antioxidants, protein, and lipid oxidation products in blood from sporadic amyotrophic lateral sclerosis patients","volume":"22","author":"Oteiza","year":"1997"},{"key":"ref28","series-title":"Exp Gerontol","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/0531-5565(87)90045-3","article-title":"Serum lipid peroxide assayed by a new colorimetric method","volume":"22","author":"Tateishi","year":"1987"},{"key":"ref29","series-title":"Vet J","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1016\/S1090-0233(97)80021-4","article-title":"Association between plasma vitamin E concentration and the risk of equine motor neuron disease","volume":"154","author":"Rua-Domenech","year":"1997"},{"key":"ref30","series-title":"J Neuropathol Exp Neurol","doi-asserted-by":"publisher","first-page":"1187","DOI":"10.1097\/NEN.0b013e31818f8f51","article-title":"The role of dietary insufficiency with antioxidants on the permeability of the blood-brain barrier","volume":"67","author":"Mohammed","year":"2008"},{"key":"ref31","series-title":"Equine Pract","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1053\/j.ctep.2006.01.005","article-title":"Equine motor neuron disease: a review of clinical and experimental studies","volume":"5","author":"Divers","year":"2006"},{"key":"ref32","series-title":"J Nutr","doi-asserted-by":"publisher","first-page":"1628","DOI":"10.1093\/jn\/132.6.1628S","article-title":"Lipoic acid as an antioxidant in mature thoroughbred geldings: a preliminary study","volume":"132","author":"Williams","year":"2002"}],"container-title":["American Journal of 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