{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T01:34:54Z","timestamp":1784252094641,"version":"3.55.0"},"reference-count":16,"publisher":"American Veterinary Medical Association (AVMA)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To evaluate the efficacy of vaccination with the <jats:italic>Leptospira interrogans<\/jats:italic> serovar <jats:italic>hardjo<\/jats:italic> type hardjoprajitno component of a pentavalent <jats:italic>Leptospira<\/jats:italic> bacterin against a virulent experimental challenge with <jats:italic>Leptospira borgpetersenii<\/jats:italic> serovar <jats:italic>hardjo<\/jats:italic> type hardjo-bovis strain 203 in cattle.<\/jats:p>\n<jats:p><jats:bold>Animals<\/jats:bold>\u2014Fifty-five 6-month-old Holstein heifers.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014Heifers that were negative for persistent infection with bovine viral diarrhea virus determined via immunohistochemical testing and negative for <jats:italic>Leptospira interrogans<\/jats:italic> serovar <jats:italic>pomona, Leptospira interrogans<\/jats:italic> serovar <jats:italic>hardjo, Leptospira interrogans<\/jats:italic> serovar <jats:italic>grippotyphosa, Leptospira interrogans<\/jats:italic> serovar <jats:italic>bratislava, Leptospira interrogans<\/jats:italic> serovar <jats:italic>canicola<\/jats:italic>, and <jats:italic>Leptospira interrogans<\/jats:italic> serovar <jats:italic>icterohaemorrhagiae<\/jats:italic> determined via microscopic agglutination assay were enrolled in the study. Two heifers were separated and used for the challenge passage. The remaining heifers were vaccinated twice with a commercial pentavalent bacterin or a sham vaccine 21 days apart and subsequently challenged with <jats:italic>L borgpetersenii<\/jats:italic> serovar <jats:italic>hardjo<\/jats:italic> type hardjo-bovis strain 203. Urinary shedding, antibody titers, and clinical signs of leptospirosis infection were recorded for 8 weeks after challenge.<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014Heifers that received the pentavalent bacterin did not shed the organism in urine after challenge and did not have renal colonization at necropsy. Heifers that were sham vaccinated shed the organism in urine and had renal colonization.<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Results provided evidence that a pentavalent <jats:italic>Leptospira<\/jats:italic> vaccine containing <jats:italic>L interrogans<\/jats:italic> serovar <jats:italic>hardjo<\/jats:italic> type hardjoprajitno can provide protection against challenge with <jats:italic>L borgpetersenii<\/jats:italic> serovar <jats:italic>hardjo<\/jats:italic> type hardjo-bovis strain 203. It is important to demonstrate cross-protection that is vaccine specific against disease-causing strains of organisms that are prevalent under field conditions.<\/jats:p>","DOI":"10.2460\/ajvr.73.5.735","type":"journal-article","created":{"date-parts":[[2012,4,25]],"date-time":"2012-04-25T18:13:49Z","timestamp":1335377629000},"page":"735-740","source":"Crossref","is-referenced-by-count":30,"title":["Efficacy of vaccination of cattle with the Leptospira interrogans serovar hardjo type hardjoprajitno component of a pentavalent Leptospira bacterin against experimental challenge with Leptospira borgpetersenii serovar hardjo type hardjo-bovis"],"prefix":"10.2460","volume":"73","author":[{"given":"Carol L.","family":"Rinehart","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicia D.","family":"Zimmerman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin E.","family":"Buterbaugh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rika A.","family":"Jolie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher C. L.","family":"Chase","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"Leptospira and leptospirosis","author":"Faine","year":"1999"},{"key":"ref2","series-title":"J Am Vet Med Assoc","first-page":"1518","article-title":"Leptospirosis","volume":"205","author":"Heath","year":"1994"},{"key":"ref3","series-title":"Vet Clin North Am Food Anim Pract","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1016\/S0749-0720(15)30532-6","article-title":"Leptospirosis as a cause of reproductive failure","volume":"10","author":"Ellis","year":"1994"},{"key":"ref4","series-title":"Am J Vet Res","first-page":"161","article-title":"Effect of vaccination with a pentavalent leptospiral vaccine on Leptospira interrogans serovar hardjo type hardjo-bovis infection of pregnant cattle","volume":"50","author":"Bolin","year":"1989"},{"key":"ref5","series-title":"Am J Vet 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