{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T21:41:52Z","timestamp":1770414112841,"version":"3.49.0"},"reference-count":49,"publisher":"American Veterinary Medical Association (AVMA)","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To assess the anesthetic efficacy and local tolerance of topically applied 0.4% oxybuprocaine ophthalmic solution to in dogs and compare its effects with those of 1% tetracaine solution.<\/jats:p>\n<jats:p><jats:bold>Animals<\/jats:bold>\u201434 ophthalmically normal Beagles.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014Dogs were assigned to 2 groups, and baseline corneal touch threshold (CTT) was measured bilaterally with a Cochet-Bonnet aesthesiometer. Dogs of group 1 (n = 22) received a single drop of 0.4% oxybuprocaine ophthalmic solution in one eye and saline (0.9% NaCl) solution (control treatment) in the contralateral eye. Dogs of group 2 (n = 12) received a single drop of 0.4% oxybuprocaine ophthalmic solution in one eye and 1% tetracaine ophthalmic solution in the contralateral eye. The CTT of each eye was measured 1 and 5 minutes after topical application and then at 5-minute intervals until 75 minutes after topical application.<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014CTT changes over time differed significantly between oxybuprocaine-treated and control eyes. After instillation of oxybuprocaine, maximal corneal anesthesia (CTT = 0) was achieved within 1 minute, and CTT was significantly decreased from 1 to 45 minutes, compared with the baseline value. No significant difference in onset, depth, and duration of corneal anesthesia was found between oxybuprocaine-treated and tetracaine-treated eyes. Conjunctival hyperemia and chemosis were detected more frequently in tetracaine-treated eyes than in oxybuprocaine-treated eyes.<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Topical application of oxybuprocaine and tetracaine similarly reduced corneal sensitivity in dogs, but oxybuprocaine was less irritating to the conjunctiva than was tetracaine.<\/jats:p>","DOI":"10.2460\/ajvr.74.10.1321","type":"journal-article","created":{"date-parts":[[2013,9,25]],"date-time":"2013-09-25T19:54:24Z","timestamp":1380138864000},"page":"1321-1326","source":"Crossref","is-referenced-by-count":23,"title":["Degree and duration of corneal anesthesia after topical application of 0.4% oxybuprocaine hydrochloride ophthalmic solution in ophthalmically normal 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antifibrotic agents, tissue adhesives, and anticollagenase agents","author":"Herring","year":"2007"},{"key":"ref3","series-title":"Textbook of ocular pharmacology","first-page":"791","article-title":"Topical and local anesthetic agents","author":"Ellis","year":"1997"},{"key":"ref4","series-title":"Goodman and Gilman's the pharmacological basis of therapeutics","first-page":"565","article-title":"Local anesthetics","author":"Catterall","year":"2010"},{"key":"ref5","series-title":"Anesthesiology","doi-asserted-by":"publisher","first-page":"711","DOI":"10.1097\/00000542-199004000-00022","article-title":"Molecular mechanisms of local anesthesia: a review","volume":"72","author":"Butterworth","year":"1990"},{"key":"ref6","series-title":"Surv Ophthalmol","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1016\/S0039-6257(01)00248-X","article-title":"Topical\/intracameral anesthesia for cataract surgery","volume":"46","author":"Hasan","year":"2001"},{"key":"ref7","series-title":"Ophthalmol Clin North Am","first-page":"155","article-title":"Pharmacology of local anesthetics","volume":"19","author":"Jackson","year":"2006"},{"key":"ref8","series-title":"Expert Opin Drug Saf","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1517\/14740338.6.6.637","article-title":"Toxicities of topical ophthalmic anesthetics","volume":"6","author":"McGee","year":"2007"},{"key":"ref9","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"459","DOI":"10.2460\/ajvr.74.3.459","article-title":"Comparison of efficacy and duration of effect on corneal sensitivity among anesthetic agents following ocular administration in clinically normal horses","volume":"74","author":"Pucket","year":"2013"},{"key":"ref10","series-title":"Veterinary ophthalmic pharmacology and therapeutics","first-page":"23","article-title":"Anesthetic agents","author":"Gelatt","year":"1987"},{"key":"ref11","series-title":"Acad Emerg Med","first-page":"364","article-title":"A comparison of proparacaine and tetracaine eye anesthetics","volume":"1","author":"Bartfield","year":"1994"},{"key":"ref12","series-title":"Arq Bras Med Vet Zootec","doi-asserted-by":"publisher","first-page":"1337","DOI":"10.1590\/S0102-09352011000600009","article-title":"Ophthalmic and anesthetic evaluation of topical 1% tetracaine and 0.5% proparacaine in dogs","volume":"63","author":"Parchen","year":"2011"},{"key":"ref13","series-title":"J Am Vet Med Assoc","doi-asserted-by":"publisher","first-page":"1645","DOI":"10.2460\/javma.241.12.1645","article-title":"Effects of action of proparacaine and tetracaine topical ophthalmic formulations on corneal sensitivity in horses","volume":"241","author":"Sharrow-Reabe","year":"2012"},{"key":"ref14","series-title":"AMA Arch Ophthalmol","doi-asserted-by":"publisher","first-page":"663","DOI":"10.1001\/archopht.1954.00920040673010","article-title":"Benoxinate (dorsacaine) for rapid corneal anesthesia","volume":"51","author":"Schlegel HE","year":"1954"},{"key":"ref15","series-title":"Am J Ophthalmol","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1016\/0002-9394(55)91114-4","article-title":"An experimental clinical evaluation of dorsacaine hydrochloride (benoxinate novesine); report on the instillation of a 0.4-percent solution","volume":"40","author":"Emmerich","year":"1955"},{"key":"ref16","series-title":"Am J Ophthalmol","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1016\/0002-9394(55)91498-7","article-title":"Topical anesthesia in ophthalmology","volume":"40","author":"Linn JG","year":"1955"},{"key":"ref17","series-title":"Clinical ophthalmic pharmacology","first-page":"335","article-title":"Local anesthetics","author":"Gandhi","year":"1987"},{"key":"ref18","series-title":"Clinical ocular pharmacology","first-page":"85","article-title":"Local anesthetics","author":"Than","year":"2008"},{"key":"ref19","series-title":"Acta Ophthalmol Scand","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1034\/j.1600-0420.1998.760518.x","article-title":"Accurate time interval in repeated tonometry","volume":"76","author":"Recep","year":"1998"},{"key":"ref20","series-title":"Anaesthesia","doi-asserted-by":"publisher","first-page":"616","DOI":"10.1111\/j.1365-2044.1992.tb02339.x","article-title":"Haemodynamic stability during general anaesthesia for intra-ocular surgery: the effect of topical oxybuprocaine","volume":"47","author":"Lytle","year":"1992"},{"key":"ref21","series-title":"Cont Lens Anterior Eye","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/j.clae.2003.11.001","article-title":"Effects of benoxinate hydrochloride 0.4% on the morphological appearance of the cornea using confocal microscopy","volume":"27","author":"Perez-Gomez","year":"2004"},{"key":"ref22","series-title":"Br J Ophthalmol","doi-asserted-by":"publisher","first-page":"847","DOI":"10.1136\/bjo.2004.056614","article-title":"A comparison of four methods of tonometry: method agreement and interobserver variability","volume":"89","author":"Tonnu","year":"2005"},{"key":"ref23","series-title":"Br J Ophthalmol","doi-asserted-by":"publisher","first-page":"1679","DOI":"10.1136\/bjo.2009.173021","article-title":"Nitrous oxide cryotherapy for primary periocular basal cell carcinoma: outcome at 5 years follow-up","volume":"95","author":"Moesen","year":"2011"},{"key":"ref24","series-title":"Vet Ophthalmol","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1046\/j.1463-5224.2001.00114.x","article-title":"The effects of topical tropicamide and systemic medetomidine, followed by atipamezole reversal, on pupil size and intraocular pressure in normal dogs","volume":"4","author":"Wallin-H\u00e5kanson","year":"2001"},{"key":"ref25","series-title":"Vet Ophthalmol","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1111\/j.1463-5224.2005.00435.x","article-title":"Ocular effects of topical 0.03% bimatoprost solution in normotensive feline eyes","volume":"9","author":"Regnier","year":"2006"},{"key":"ref26","series-title":"Bull Soc Ophtalmol Fr","first-page":"541","article-title":"Corneal esthesiometry. Performance and practical importance","volume":"6","author":"Cochet","year":"1961"},{"key":"ref27","series-title":"Prog Retin Eye Res","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/S1350-9462(96)00027-4","article-title":"Neurobiology of ocular pain","volume":"16","author":"Belmonte","year":"1997"},{"key":"ref28","series-title":"Vet Comp Ophthalmol","first-page":"245","article-title":"Absolute corneal sensitivity and corneal trigeminal nerve anatomy in normal dogs","volume":"1","author":"Barrett","year":"1991"},{"key":"ref29","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"7","DOI":"10.2460\/ajvr.2003.64.7","article-title":"Corneal sensitivity in dogs with diabetes mellitus","volume":"64","author":"Good","year":"2003"},{"key":"ref30","series-title":"Veterinary ophthalmology","first-page":"438","article-title":"The eye examination and diagnostic procedure","author":"Ollivier","year":"2007"},{"key":"ref31","series-title":"Vet Ophthalmol","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1111\/j.1463-5224.2012.01069.x","article-title":"Correlation between corneal sensitivity and quantity of reflex tearing in cows, horses, goats, sheep, dogs, cats, rabbits, and guinea pigs","volume":"16","author":"Wieser","year":"2013"},{"key":"ref32","series-title":"Dtsch Med Wochenschr","first-page":"212","article-title":"Die Sensibilit\u00e4t der Hornhaut und Bindehaut des menschlichen Auges","volume":"48","author":"Von Frey","year":"1922"},{"key":"ref33","series-title":"Optom Vis Sci","doi-asserted-by":"publisher","first-page":"1025","DOI":"10.1097\/00006324-199712000-00024","article-title":"Effect of 0.4% benoxinate hydrochloride on corneal sensitivity, measured using the non-contact corneal aesthesiometer (NCCA)","volume":"74","author":"Murphy","year":"1997"},{"key":"ref34","series-title":"Acta Ophthalmol (Copenh)","first-page":"434","article-title":"Effect of oxygen deprivation on corneal sensitivity","volume":"58","author":"Millodot","year":"1980"},{"key":"ref35","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"77","DOI":"10.2460\/ajvr.2005.66.77","article-title":"Duration of effect and effect of multiple doses of topical ophthalmic 0.5% proparacaine hydrochloride in clinically normal dogs","volume":"66","author":"Herring","year":"2005"},{"key":"ref36","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"1780","DOI":"10.2460\/ajvr.67.10.1780","article-title":"Duration of corneal anesthesia following topical administration of 0.5% proparacaine hydrochloride solution in clinically normal cats","volume":"67","author":"Binder","year":"2006"},{"key":"ref37","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"1655","DOI":"10.2460\/ajvr.69.12.1655","article-title":"Evaluation of duration of corneal anesthesia induced with ophthalmic 0.5% proparacaine hydrochloride by use of a Cochet-Bonnet aesthesiometer in clinically normal horses","volume":"69","author":"Kalf","year":"2008"},{"key":"ref38","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"223","DOI":"10.2460\/ajvr.71.2.223","article-title":"Effect of treatment with a topical ophthalmic preparation of 1% nalbuphine solution on corneal sensitivity in clinically normal horses","volume":"71","author":"Wotman","year":"2010"},{"key":"ref39","series-title":"Equine Vet J","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1111\/j.2042-3306.2010.00118.x","article-title":"Duration of corneal anaesthesia following multiple doses and two concentrations of tetracaine hydrochloride eyedrops on the normal equine cornea","volume":"43","author":"Monclin","year":"2011"},{"key":"ref40","series-title":"Br J Ophthalmol","doi-asserted-by":"publisher","first-page":"588","DOI":"10.1136\/bjo.80.7.588","article-title":"Control of microbial contamination in unpreserved eyedrops","volume":"80","author":"Oldham","year":"1996"},{"key":"ref41","series-title":"Ophthalmic Physiol Opt","doi-asserted-by":"publisher","first-page":"393","DOI":"10.1046\/j.1475-1313.1998.00381.x","article-title":"Comparison of the tolerability and efficacy of unit-dose, preservative-free topical ocular anaesthetics","volume":"18","author":"Lawrenson","year":"1998"},{"key":"ref42","series-title":"Contact Lens","first-page":"5","article-title":"Effect of bending of the nylon thread of the Cochet-Bonnet corneal aesthesiometer upon the recorded pressure","volume":"1","author":"Millodot","year":"1967"},{"key":"ref43","series-title":"Acta Ophthalmol (Copenh)","first-page":"789","article-title":"Corneal sensivity by low temperatures","volume":"48","author":"Kolstad","year":"1970"},{"key":"ref44","series-title":"Am J Optom Physiol Opt","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1097\/00006324-197801000-00002","article-title":"Dose-response effects of corneal anesthetics","volume":"55","author":"Polse","year":"1978"},{"key":"ref45","series-title":"J Am Vet Med Assoc","first-page":"15","article-title":"Ocular sensitivity of dogs to topical tetracaine HCl","volume":"154","author":"Koch","year":"1969"},{"key":"ref46","series-title":"J Am Vet Med Assoc","first-page":"1449","article-title":"Corneal epithelial sloughing in dogs with glaucoma","volume":"151","author":"Rubin","year":"1967"},{"key":"ref47","series-title":"Ophthalmic Physiol Opt","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1111\/j.1475-1313.1997.0_841.x","article-title":"Eye drop container delivery: a source of response variation?","volume":"17","author":"German","year":"1997"},{"key":"ref48","series-title":"Eye (Lond)","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1038\/eye.1999.17","article-title":"Reliability of drop size from multi-dose eye drop bottles: is it cause for concern?","volume":"13","author":"German","year":"1999"},{"key":"ref49","series-title":"Surv Ophthalmol","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1016\/j.survophthal.2003.12.009","article-title":"Determinants of eye drop size","volume":"49","author":"Van Santvliet","year":"2004"}],"container-title":["American Journal of Veterinary 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