{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T16:42:42Z","timestamp":1758127362232,"version":"3.38.0"},"reference-count":47,"publisher":"American Veterinary Medical Association (AVMA)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To determine whether 14-day topical ocular administration of high doses of feline recombinant interferon omega (FelFN) or human recombinant interferon alpha-2b (HulFN) solution improves clinical disease and decreases virus shedding in cats with naturally acquired viral keratoconjunctivitis.<\/jats:p>\n<jats:p><jats:bold>Animals<\/jats:bold>\u201436 cats with upper respiratory tract disease and ocular involvement.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014Cats received 1 drop of FelFN solution (1 \u00d7 10<jats:sup>6<\/jats:sup> U\/mL), HulFN solution (1 \u00d7 10<jats:sup>6<\/jats:sup> U\/mL), or saline (0.9% NaCl) solution (12 cats\/group) in each eye twice daily for 14 days (beginning day 1). Oropharyngeal and conjunctival swab samples were collected from each cat before (day 0) and on day 14 of treatment for virus isolation (VI) and real-time quantitative PCR (RT-qPCR) testing to detect feline herpesvirus-1 and feline calicivirus. Subjective clinical scores were recorded on days 0, 3, 7, 10, and 14.<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014The number of cats for which feline herpesvirus-1 was detected via VI or RT-qPCR assay was generally (albeit not always significantly) lower on day 14, compared with day 0 findings; however, findings on days 0 or 14 did not differ among groups. The number of cats for which feline calicivirus was detected via VI or RT-qPCR assay did not differ significantly between days 0 and 14 for any group. Clinical scores significantly decreased over the 14-day period but did not differ among groups.<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014In cats with naturally occurring viral keratoconjunctivitis, bilateral ocular administration of high doses of FelFN or HulFN twice daily for 14 days did not improve clinical disease or virus shedding, compared with treatment with saline solution.<\/jats:p>","DOI":"10.2460\/ajvr.74.2.281","type":"journal-article","created":{"date-parts":[[2013,1,30]],"date-time":"2013-01-30T18:41:44Z","timestamp":1359571304000},"page":"281-289","source":"Crossref","is-referenced-by-count":17,"title":["Effects of topical ocular administration of high doses of human recombinant interferon alpha-2b and feline recombinant interferon omega on naturally occurring viral keratoconjunctivitis in cats"],"prefix":"10.2460","volume":"74","author":[{"given":"Jessica M.","family":"Slack","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean","family":"Stiles","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian M.","family":"Leutenegger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George E.","family":"Moore","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roman M.","family":"Pogranichniy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"Vet Clin North Am Small Anim Pract","doi-asserted-by":"publisher","first-page":"1001","DOI":"10.1016\/S0195-5616(00)05003-8","article-title":"Feline herpesvirus","volume":"30","author":"Stiles","year":"2000"},{"key":"ref2","series-title":"Compend Contin Educ Pract Vet","first-page":"166","article-title":"Feline upper respiratory tract pathogens: herpesvirus-1 and calicivirus","volume":"23","author":"Sykes","year":"2001"},{"key":"ref3","series-title":"J Feline Med Surg","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1016\/j.jfms.2008.11.012","article-title":"Treatment of feline herpesvirus-1 associated disease in cats with famciclovir and related drugs","volume":"11","author":"Malik","year":"2009"},{"key":"ref4","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"99","DOI":"10.2460\/AJVR.2002.63.99","article-title":"Effect of oral administration of L-lysine on conjunctivitis caused by feline herpesvirus in cats","volume":"63","author":"Stiles","year":"2002"},{"key":"ref5","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"289","DOI":"10.2460\/ajvr.69.2.289","article-title":"Effect of topical ophthalmic application of cidofovir on experimentally induced primary ocular feline herpesvirus-1 infection in cats","volume":"69","author":"Fontenelle","year":"2008"},{"key":"ref6","series-title":"Am J Vet Res","first-page":"158","article-title":"In vitro susceptibility of feline herpesvirus-1 to virabidine, idoxuridine, trifluridine, acyclovir or bromovinyldeoxyuridine","volume":"50","author":"Nasisse","year":"1989"},{"key":"ref7","series-title":"Clin Tech Small Anim Pract","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1016\/S1096-2867(03)90014-4","article-title":"Feline herpesvirus","volume":"18","author":"Stiles","year":"2003"},{"key":"ref8","series-title":"J Gen Virol","doi-asserted-by":"publisher","first-page":"2341","DOI":"10.1099\/0022-1317-81-10-2341","article-title":"Interferons: cell signalling, immune modulation, antiviral responses and virus countermeasures","volume":"81","author":"Goodbuourn","year":"2000"},{"key":"ref9","series-title":"J Clin Microbiol","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1128\/JCM.40.2.519-523.2002","article-title":"Quantification of feline herpesvirus-1 DNA in ocular samples of clinically diseased cats by real time TaqMan PCR","volume":"40","author":"V\u00f6gtlin","year":"2002"},{"key":"ref10","series-title":"Vet Rec","doi-asserted-by":"publisher","first-page":"857","DOI":"10.1136\/vr.162.26.857","article-title":"Nucleic acid extraction methods for detection of EHV-1 from blood and nasopharyngeal secretions","volume":"162","author":"Mapes","year":"2008"},{"key":"ref11","series-title":"Vet Rec","doi-asserted-by":"publisher","first-page":"272","DOI":"10.1136\/vr.161.8.272","article-title":"Evaluation of nasopharyngeal swabs and feces as potentially useful diagnostic specimens for Rhodococcus equi pneumonia in foals using real-time TaqMan PCR","volume":"161","author":"Pusterla","year":"2007"},{"key":"ref12","series-title":"Proc R Soc Lond (Biol)","first-page":"258","article-title":"Virus interference. 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