{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T09:41:13Z","timestamp":1771234873298,"version":"3.50.1"},"reference-count":36,"publisher":"American Veterinary Medical Association (AVMA)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To characterize systemic immune responses in <jats:italic>Cytauxzoon felis-infected<\/jats:italic> cats.<\/jats:p>\n<jats:p><jats:bold>Sample<\/jats:bold>\u2014Blood and lung samples obtained from 27 cats.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014Cats were allocated into 4 groups: cats that died of cytauxzoonosis, acutely ill <jats:italic>C felis-infected<\/jats:italic> cats, healthy survivors of <jats:italic>C felis<\/jats:italic> infection, and healthy uninfected cats. Serum concentrations of tumor necrosis factor-\u03b1 and interleukin-1 \u03b2 were measured and serum proteins characterized. Blood smears were stained immunocytochemically and used to assess immunoglobulin deposition. Immunohistochemical expression of CD18 and tumor necrosis factor-\u03b1 were compared in lung tissues obtained from cats that died and healthy uninfected cats. A real-time reverse-transcription PCR assay for CD18 expression was performed on selected blood samples from all groups.<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014Concentrations of both cytokines were greater and serum albumin concentrations were significantly lower in cats that died of cytauxzoonosis, compared with results for all other groups. Erythrocytes from acutely ill cats and survivors of <jats:italic>C felis<\/jats:italic> infection had staining for plasmalemmal IgM, whereas erythrocytes from the other groups did not. Increased staining of <jats:italic>C felis<\/jats:italic>-infected monocytes and interstitial neutrophils for CD18 was detected. The real-time reverse-transcription PCR assay confirmed a relative increase in CD18 expression in cats that died of cytauxzoonosis and acutely ill cats, compared with expression in other groups. Immunostaining for TNF-\u03b1 in lung samples confirmed a local proinflammatory response.<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Results indicated immunopathologic responses were greater in cats that died of <jats:italic>C felis<\/jats:italic> infection than in cats that survived <jats:italic>C felis<\/jats:italic> infection.<\/jats:p>","DOI":"10.2460\/ajvr.74.6.901","type":"journal-article","created":{"date-parts":[[2013,5,29]],"date-time":"2013-05-29T15:21:11Z","timestamp":1369840871000},"page":"901-909","source":"Crossref","is-referenced-by-count":11,"title":["Systemic immune responses in Cytauxzoon felis-infected domestic cats"],"prefix":"10.2460","volume":"74","author":[{"given":"Karelma","family":"Frontera-Acevedo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole M.","family":"Balsone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melissa A.","family":"Dugan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheryl R.","family":"Makemson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Llewelyn B.","family":"Sellers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Holly M.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David S.","family":"Peterson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kate E.","family":"Creevy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bridget C.","family":"Garner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaori","family":"Sakamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"Cytauxzoonosis","first-page":"243","year":"2007"},{"key":"ref2","series-title":"Cytauxzoonosis","first-page":"716","author":"Greene","year":"2006"},{"key":"ref3","series-title":"Chapter 13: bone marrow, blood cells, and lymphatic system","first-page":"784","author":"Zachary","year":"2012"},{"key":"ref4","series-title":"Transmission of Cytauxzoon felis to a domestic cat by Amblyomma americanum","first-page":"110","volume":"161","author":"Reichard","year":"2009"},{"key":"ref5","series-title":"Detection of anticytauxzoon antibodies in cats infected with a Cytauxzoon organism from bobcats","first-page":"43","volume":"28","author":"Cowell","year":"1988"},{"key":"ref6","series-title":"The 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