{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T20:31:28Z","timestamp":1787776288588,"version":"build-2784847793"},"reference-count":51,"publisher":"American Veterinary Medical Association (AVMA)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>OBJECTIVE<\/jats:bold> To investigate regional differences of relative metabolite concentrations in the brain of healthy dogs with short echo time, single voxel proton magnetic resonance spectroscopy (<jats:sup>1<\/jats:sup>H MRS) at 3.0 T.<\/jats:p>\n<jats:p><jats:bold>ANIMALS<\/jats:bold> 10 Beagles.<\/jats:p>\n<jats:p><jats:bold>PROCEDURES<\/jats:bold> Short echo time, single voxel <jats:sup>1<\/jats:sup>H MRS was performed at the level of the right and left basal ganglia, right and left thalamus, right and left parietal lobes, occipital lobe, and cerebellum. Data were analyzed with an automated fitting method (linear combination model). Metabolite concentrations relative to water content were obtained, including <jats:italic>N<\/jats:italic>-acetyl aspartate, total choline, creatine, myoinositol, the sum of glutamine and glutamate (glutamine-glutamate complex), and glutathione. Metabolite ratios with creatine as the reference metabolite were calculated. Concentration differences between right and left hemispheres and sexes were evaluated with a Wilcoxon signed rank test and among various regions of the brain with an independent <jats:italic>t<\/jats:italic> test and 1-way ANOVA.<\/jats:p>\n<jats:p><jats:bold>RESULTS<\/jats:bold> No significant differences were detected between sexes and right and left hemispheres. All metabolites, except the glutamine-glutamate complex and glutathione, had regional concentrations that differed significantly. The creatine concentration was highest in the basal ganglia and cerebellum and lowest in the parietal lobes. The <jats:italic>N<\/jats:italic>-acetyl aspartate concentration was highest in the parietal lobes and lowest in the cerebellum. Total choline concentration was highest in the basal ganglia and lowest in the occipital lobe.<\/jats:p>\n<jats:p><jats:bold>CONCLUSIONS AND CLINICAL RELEVANCE<\/jats:bold> Metabolite concentrations differed among brain parenchymal regions in healthy dogs. This study may provide reference values for clinical and research studies involving <jats:sup>1<\/jats:sup>H MRS performed at 3.0 T.<\/jats:p>","DOI":"10.2460\/ajvr.76.2.129","type":"journal-article","created":{"date-parts":[[2015,1,28]],"date-time":"2015-01-28T19:03:18Z","timestamp":1422471798000},"page":"129-141","source":"Crossref","is-referenced-by-count":15,"title":["Regional metabolite concentrations in the brain of healthy dogs measured by use of short echo time, single voxel proton magnetic resonance spectroscopy at 3.0 Tesla"],"prefix":"10.2460","volume":"76","author":[{"given":"In\u00e9s","family":"Carrera","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henning","family":"Richter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieter","family":"Meier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick 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Imaging","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1097\/RMR.0b013e31821e568f","article-title":"Quantitative proton magnetic resonance spectroscopy and spectroscopic imaging of the brain: a didactic review","volume":"21","author":"Alger","year":"2010"},{"key":"ref5","series-title":"NMR Biomed","doi-asserted-by":"publisher","first-page":"617","DOI":"10.1002\/nbm.1060","article-title":"Quantification of MRS data in the frequency domain using a wavelet filter, an approximated Voigt lineshape model and prior knowledge","volume":"19","author":"Gillies","year":"2006"},{"key":"ref6","series-title":"J Magn Reson","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/j.jmr.2004.01.015","article-title":"Subspace-based MRS data quantitation of multiplets using prior knowledge","volume":"168","author":"Laudadio","year":"2004"},{"key":"ref7","series-title":"NMR Biomed","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1002\/nbm.697","article-title":"MR spectroscopy quantitation: a review of frequency domain methods","volume":"14","author":"Mierisov\u00e1","year":"2001"},{"key":"ref8","series-title":"NMR Biomed","doi-asserted-by":"publisher","first-page":"260","DOI":"10.1002\/nbm.698","article-title":"Automatic quantitation of localized in vivo 1H spectra with LCModel","volume":"14","author":"Provencher","year":"2001"},{"key":"ref9","series-title":"Magn Reson Med","doi-asserted-by":"publisher","first-page":"822","DOI":"10.1002\/mrm.1910400607","article-title":"Automated spectral analysis III: application to in vivo proton MR spectroscopy and spectroscopic imaging","volume":"40","author":"Soher","year":"1998"},{"key":"ref10","series-title":"J Magn Reson","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1006\/jmre.1999.1835","article-title":"Time-domain quantification of series of biomedical magnetic resonance spectroscopy signals","volume":"140","author":"Vanhamme","year":"1999"},{"key":"ref11","series-title":"Magn Reson Med","doi-asserted-by":"publisher","first-page":"672","DOI":"10.1002\/mrm.1910300604","article-title":"Estimation of metabolite concentrations from localized in vivo proton NMR spectra","volume":"30","author":"Provencher","year":"1993"},{"key":"ref12","series-title":"Radiology","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1148\/radiol.2402050314","article-title":"1H MR spectroscopy of the brain: absolute quantification of metabolites","volume":"240","author":"Jansen","year":"2006"},{"key":"ref13","series-title":"Magn Reson Imaging","doi-asserted-by":"publisher","first-page":"923","DOI":"10.1016\/S0730-725X(03)00181-4","article-title":"Metabolite ratios to assumed stable creatine level may confound the quantification of proton brain MR spectroscopy","volume":"21","author":"Li","year":"2003"},{"key":"ref14","series-title":"NMR Biomed","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1002\/nbm.1940040203","article-title":"A review of chemical issues in 1H NMR spectroscopy:N-acetyl-l-aspartate, creatine and choline","volume":"4","author":"Miller","year":"1991"},{"key":"ref15","series-title":"J Magn Reson Imaging","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1002\/jmri.21285","article-title":"Regional apparent metabolite concentrations in young adult brain measured by 1H MR spectroscopy at 3 Tesla","volume":"27","author":"Baker","year":"2008"},{"key":"ref16","series-title":"Magn Reson Med","doi-asserted-by":"publisher","first-page":"699","DOI":"10.1002\/mrm.1248","article-title":"Quantitative proton MR spectroscopic imaging of normal human cerebellum and brain stem","volume":"46","author":"Jacobs","year":"2001"},{"key":"ref17","series-title":"Psychiatry Res","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1016\/j.pscychresns.2006.11.005","article-title":"In vivo metabolite differences between the basal ganglia and cerebellum of the rat brain detected with proton MRS at 3T","volume":"154","author":"Mayer","year":"2007"},{"key":"ref18","series-title":"Eur J Radiol","doi-asserted-by":"publisher","first-page":"e223","DOI":"10.1016\/j.ejrad.2011.01.106","article-title":"Age-related differences in metabolites in the posterior cingulate cortex and hippocampus of normal ageing brain: a 1H-MRS study","volume":"81","author":"Reyngoudt","year":"2012"},{"key":"ref19","series-title":"NMR Biomed","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1002\/nbm.701","article-title":"Cramer-Rao bounds: an evaluation tool for quantitation","volume":"14","author":"Cavassila","year":"2001"},{"key":"ref20","series-title":"NMR Biomed","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1002\/nbm.891","article-title":"Issues of spectral quality in clinical 1H-magnetic resonance spectroscopy and a gallery of artifacts","volume":"17","author":"Kreis","year":"2004"},{"key":"ref21","series-title":"ScientificWorldJournal","first-page":"546171","article-title":"Spectroscopic evaluation of glioma grading at 3T: the combined role of short and long TE","volume":"2012","author":"Kousi","year":"2012"},{"key":"ref22","series-title":"Magn Reson Med","doi-asserted-by":"publisher","first-page":"1279","DOI":"10.1002\/mrm.21961","article-title":"MR spectroscopy of the human brain with enhanced signal intensity at ultrashort echo times on a clinical platform at 3T and 7T","volume":"61","author":"Mekle","year":"2009"},{"key":"ref23","series-title":"Magn Reson Imaging","doi-asserted-by":"publisher","first-page":"1159","DOI":"10.1016\/S0730-725X(00)00212-5","article-title":"Short TE in vivo 1H MR spectroscopic imaging at 1.5 T: acquisition and automated spectral analysis","volume":"18","author":"Soher","year":"2000"},{"key":"ref24","series-title":"Int J Neurosci","doi-asserted-by":"publisher","first-page":"537","DOI":"10.3109\/00207454.2013.774395","article-title":"3 Tesla magnetic resonance spectroscopy: cerebral gliomas vs. metastatic brain tumors. Our experience and review of the literature","volume":"123","author":"Caivano","year":"2013"},{"key":"ref25","series-title":"J Neuroimmune Pharmacol","doi-asserted-by":"publisher","first-page":"576","DOI":"10.1007\/s11481-013-9460-x","article-title":"Magnetic resonance spectroscopy to assess neuroinflammation and neuropathic pain","volume":"8","author":"Chang","year":"2013"},{"key":"ref26","series-title":"Neurol Neurochir Pol","doi-asserted-by":"publisher","first-page":"247","DOI":"10.5114\/ninp.2013.32998","article-title":"Differential diagnosis of intracranial meningiomas based on magnetic resonance spectroscopy","volume":"47","author":"Jask\u00f3lski","year":"2013"},{"key":"ref27","series-title":"Eur J Radiol","doi-asserted-by":"publisher","first-page":"250","DOI":"10.1016\/j.ejrad.2008.02.033","article-title":"1H MR spectroscopy of inflammation, infection and ischemia of the brain","volume":"67","author":"Mader","year":"2008"},{"key":"ref28","series-title":"Neuroradiology","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1007\/s00234-001-0760-0","article-title":"Clinical application of proton magnetic resonance spectroscopy in the diagnosis of intracranial mass lesions","volume":"44","author":"M\u00f6ller-Hartmann","year":"2002"},{"key":"ref29","series-title":"Magn Reson Med Sci","doi-asserted-by":"publisher","first-page":"167","DOI":"10.2463\/mrms.9.167","article-title":"Clinical application of MR spectroscopy and imaging of brain tumor","volume":"9","author":"Morita","year":"2010"},{"key":"ref30","series-title":"Pediatr Radiol","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1007\/s00247-009-1450-z","article-title":"Magnetic resonance spectroscopy in pediatric neuroradiology: clinical and research applications","volume":"40","author":"Panigrahy","year":"2010"},{"key":"ref31","series-title":"Neuroimaging Clin North Am","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1016\/j.nic.2012.12.013","article-title":"Magnetic resonance spectroscopy in metabolic disorders","volume":"23","author":"Rossi","year":"2013"},{"issue":"11","key":"ref32","series-title":"PLoS ONE","doi-asserted-by":"publisher","first-page":"e49272","DOI":"10.1371\/journal.pone.0049272","article-title":"Change in brain magnetic resonance spectroscopy after treatment during acute HIV infection","volume":"7","author":"Sailasuta","year":"2012"},{"key":"ref33","series-title":"Adv Neurol","first-page":"185","article-title":"The role of MRS and fMRI in multiple sclerosis","volume":"98","author":"Tartaglia","year":"2006"},{"key":"ref34","series-title":"Invest Radiol","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1097\/00004424-199406000-00001","article-title":"Multimodality correlative study of canine brain tumors. 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normal cats","volume":"23","author":"Magnitsky","year":"2010"},{"key":"ref38","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"818","DOI":"10.2460\/ajvr.75.9.818","article-title":"In vivo proton magnetic resonance spectroscopy for the evaluation of hepatic encephalopathy in dogs","volume":"75","author":"Carrera","year":"2014"},{"key":"ref39","series-title":"Vet Radiol Ultrasound","doi-asserted-by":"publisher","first-page":"S23","DOI":"10.1111\/j.1740-8261.2010.01782.x","article-title":"Correlating magnetic resonance findings with neuropathology and clinical signs in dogs and cats","volume":"52","author":"Vite","year":"2011"},{"key":"ref40","series-title":"Neurobiol Aging","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1016\/j.neurobiolaging.2007.07.005","article-title":"A systematic review of brain metabolite changes, measured with 1H magnetic resonance spectroscopy, in healthy aging","volume":"30","author":"Haga","year":"2009"},{"key":"ref41","series-title":"Magn Reson Imaging","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1016\/S0730-725X(98)00186-6","article-title":"Effects of gender and region on proton MRS of normal human brain","volume":"17","author":"Komoroski","year":"1999"},{"key":"ref42","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"179","DOI":"10.2460\/ajvr.75.2.179","article-title":"Regional variations and age-related changes detected with magnetic resonance spectroscopy in the brain of healthy dogs","volume":"75","author":"Ono","year":"2014"},{"key":"ref43","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"1291","DOI":"10.2460\/ajvr.74.10.1291","article-title":"Relative metabolite concentrations and ratios determined by use of 3-T region-specific proton magnetic resonance spectroscopy of the brain of healthy Beagles","volume":"74","author":"Warrington","year":"2013"},{"key":"ref44","series-title":"Radiology","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1148\/radiology.187.1.8451417","article-title":"Absolute concentrations of metabolites in the adult human brain in vivo: quantification of localized proton MR spectra","volume":"187","author":"Michaelis","year":"1993"},{"key":"ref45","series-title":"Magn Reson Med","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1002\/mrm.1910390110","article-title":"Regional metabolite concentrations in human brain as determined by quantitative localized proton MRS","volume":"39","author":"Pouwels","year":"1998"},{"key":"ref46","series-title":"NMR Biomed","doi-asserted-by":"publisher","first-page":"1044","DOI":"10.1002\/nbm.1529","article-title":"Improvement of resolution for brain coupled metabolites by optimized 1H MRS at 7T","volume":"23","author":"Choi","year":"2010"},{"key":"ref47","series-title":"NMR Biomed","doi-asserted-by":"publisher","first-page":"888","DOI":"10.1002\/nbm.1646","article-title":"Noninvasive quantification of ascorbate and glutathione concentration in the elderly human brain","volume":"24","author":"Emir","year":"2011"},{"key":"ref48","series-title":"Psychopharmacology (Berl)","article-title":"Neurochemistry of major depression: a study using magnetic resonance spectroscopy [Published online ahead of print Jul 31, 2014]","author":"Godlewska"},{"key":"ref49","series-title":"J Magn Reson Imaging","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1002\/jmri.10429","article-title":"Asymmetry and gender effect in functionally lateralized cortical regions: a proton MRS imaging study","volume":"19","author":"Nagae-Poetscher","year":"2004"},{"key":"ref50","series-title":"Magn Reson Med","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1002\/mrm.1183","article-title":"Analysis of volume MRI and MR spectroscopic imaging data for the evaluation of patients with brain tumors","volume":"46","author":"Nelson","year":"2001"},{"key":"ref51","series-title":"Prog Neuropsychopharmacol Biol Psychiatry","doi-asserted-by":"publisher","first-page":"995","DOI":"10.1016\/0278-5846(94)90125-2","article-title":"Proton spectroscopy of human brain: effects of age and sex","volume":"18","author":"Charles","year":"1994"}],"container-title":["American Journal of Veterinary 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