{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:03:41Z","timestamp":1770746621822,"version":"3.49.0"},"reference-count":18,"publisher":"American Veterinary Medical Association (AVMA)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>OBJECTIVE<\/jats:bold> To determine whether plasma ACTH concentrations vary following administration of a thyrotropin-releasing hormone (TRH) solution prepared for research purposes and stored at \u221220\u00b0C (rTRH) or prepared by a compounding pharmacy and stored at room temperature (approx 22\u00b0C; cTRH).<\/jats:p>\n<jats:p><jats:bold>ANIMALS<\/jats:bold> 34 adult horses.<\/jats:p>\n<jats:p><jats:bold>PROCEDURES<\/jats:bold> The study consisted of 2 experiments. In experiment 1, each horse underwent 2 TRH stimulation tests separated by 24 hours; 10 horses were administered cTRH for the first test and rTRH for the second test (group 1), 10 horses were administered rTRH for the first test and cTRH for the second test (group 2), and 10 horses were administered rTRH for both tests (group 3). Plasma ACTH concentrations were measured at 0 (baseline) and 30 minutes after TRH administration and the delta ACTH responses (change in ACTH concentration after TRH administration) were calculated. In experiment 2, the design was the same as that for experiment 1 except there were 14 days between tests, ACTH was measured at 0 and 10 minutes after TRH administration, and 11, 9, and 10 horses were assigned to groups 1, 2, and 3, respectively.<\/jats:p>\n<jats:p><jats:bold>RESULTS<\/jats:bold> Adverse effects associated with TRH administration included transient coughing and yawning. In experiment 1, the median delta ACTH response for the second test was significantly lower than that for the first test for all groups. In experiment 2, the median delta ACTH response did not differ significantly between the first and second tests for any group, ACTH concentrations after rTRH administration were positively correlated (<jats:italic>r<\/jats:italic><jats:sub>s<\/jats:sub> = 0.95) with those after cTRH administration, and the mean \u00b1 SD bias in post-TRH ACTH concentration between rTRH and cTRH was 2.9 \u00b1 12.4 pg\/mL.<\/jats:p>\n<jats:p><jats:bold>CONCLUSIONS AND CLINICAL RELEVANCE<\/jats:bold> Results indicated that the TRH stimulation test should not be repeated within 24 hours, and cTRH solution stored at room temperature could be used to effectively perform TRH stimulation testing in horses.<\/jats:p>","DOI":"10.2460\/ajvr.76.5.437","type":"journal-article","created":{"date-parts":[[2015,4,24]],"date-time":"2015-04-24T16:07:46Z","timestamp":1429891666000},"page":"437-444","source":"Crossref","is-referenced-by-count":10,"title":["Evaluation of a thyrotropin-releasing hormone solution stored at 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Endocrinol","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/j.domaniend.2014.02.004","article-title":"Influence of feeding status, time of the day, and season on baseline adrenocorticotropic hormone and the response to thyrotropin releasing hormone-stimulation test in healthy horses","volume":"48","author":"Diez de Castro","year":"2014"},{"key":"ref15","series-title":"Endocrinology","doi-asserted-by":"publisher","first-page":"1430","DOI":"10.1210\/endo-110-4-1430","article-title":"Equine Cushing's disease: plasma immunoreactive proopiolipomelanocortin peptide and cortisol levels basally and in response to diagnostic tests","volume":"110","author":"Orth","year":"1982"},{"key":"ref16","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"1431","DOI":"10.1111\/j.1939-1676.2011.00810.x","article-title":"Comparison of cortisol and ACTH responses after administration of thyrotropin releasing hormone in normal horses and those with pituitary pars 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