{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T21:24:45Z","timestamp":1774041885380,"version":"3.50.1"},"reference-count":57,"publisher":"American Veterinary Medical Association (AVMA)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>OBJECTIVE<\/jats:bold> To assess the in vitro and in vivo platelet function of healthy dogs during administration of a low-dose aspirin regimen.<\/jats:p>\n<jats:p><jats:bold>ANIMALS<\/jats:bold> 16 dogs.<\/jats:p>\n<jats:p><jats:bold>PROCEDURES<\/jats:bold> Dogs received aspirin (1 mg\/kg, PO, q 24 h) for 7 days. Blood and urine samples were collected before (day 1; baseline) and on days 3 and 7 of the low-dose aspirin regimen. Platelet function was evaluated by use of turbidimetric and conventional impedance aggregometry, multiple-electrode impedance aggregometry, a platelet function analyzer (PFA), and determination of urine 11-dehydro-thromboxane B<jats:sub>2<\/jats:sub> concentration. Turbidimetric aggregometry results were compared with the results obtained by the other 4 methods. Fourteen days after cessation of aspirin, platelet-rich plasma was incubated with acetylsalicylic acid and platelet function was assessed by turbidimetric aggregometry to determine whether this technique could accurately identify dogs that responded to the low-dose aspirin regimen.<\/jats:p>\n<jats:p><jats:bold>RESULTS<\/jats:bold> Of the 16 dogs, 13 had turbidimetric and conventional impedance aggregometry results that were decreased by &gt; 25% from baseline on days 3 and 7, and 4 and 7 dogs had PFA closure times &gt; 300 seconds on days 3 and 7, respectively. The median urine 11-dehydro-thromboxane B<jats:sub>2<\/jats:sub> concentration\u2013to\u2013creatinine concentration ratio decreased by 49% between days 1 and 7. Turbidimetric aggregometry results were correlated with conventional impedance aggregometry results. There was poor agreement between the turbidimetric aggregometry and PFA results. The multiple-electrode impedance aggregometry protocol failed to reliably detect aspirin-induced platelet dysfunction. In vitro incubation of platelet-rich plasma with acetylsalicylic acid followed by turbidimetric aggregometry did not predict whether dogs responded to the low-dose aspirin regimen.<\/jats:p>\n<jats:p><jats:bold>CONCLUSIONS AND CLINICAL RELEVANCE<\/jats:bold> Results indicated that the response to a low-dose aspirin regimen varied among healthy dogs. 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1985\u20131995","volume":"13","author":"Johnson","year":"1999"},{"key":"ref3","series-title":"Chest","doi-asserted-by":"publisher","first-page":"39S","DOI":"10.1378\/chest.119.1_suppl.39S","article-title":"Platelet-active drugs: the relationships among dose, effectiveness, and side effects","volume":"119","author":"Patrono","year":"2001"},{"key":"ref4","series-title":"J Am Vet Med Assoc","doi-asserted-by":"publisher","first-page":"1869","DOI":"10.2460\/javma.2005.226.1869","article-title":"Evaluation of prognostic factors, survival rates, and treatment protocols for immune-mediated hemolytic anemia in dogs: 151 cases (1993\u20132002)","volume":"226","author":"Weinkle","year":"2005"},{"key":"ref5","series-title":"J Thromb Haemost","doi-asserted-by":"publisher","first-page":"1710","DOI":"10.1046\/j.1538-7836.2003.00284.x","article-title":"Aspirin resistance: definition, mechanisms and clinical read-outs","volume":"1","author":"Patrono","year":"2003"},{"key":"ref6","series-title":"Pharmacotherapy","doi-asserted-by":"publisher","first-page":"942","DOI":"10.1592\/phco.2005.25.7.942","article-title":"Aspirin resistance: an evaluation of current evidence and measurement methods","volume":"25","author":"Martin","year":"2005"},{"key":"ref7","series-title":"Circulation","doi-asserted-by":"publisher","first-page":"2888","DOI":"10.1161\/CIRCULATIONAHA.105.596627","article-title":"Residual arachidonic acid-induced platelet activation via an adenosine diphosphate-dependent but cyclooxygenase-1- and cyclooxygenase-2-independent pathway: a 700-patient study of aspirin resistance","volume":"113","author":"Frelinger AL","year":"2006"},{"key":"ref8","series-title":"JK Sci","first-page":"116","article-title":"Aspirin resistance","volume":"8","author":"Kour","year":"2006"},{"key":"ref9","series-title":"Life Sci","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1016\/j.lfs.2010.10.017","article-title":"Inducible COX-2 dominates over COX-1 in prostacyclin biosynthesis: mechanisms of COX-2 inhibitor risk to heart disease","volume":"88","author":"Ruan","year":"2011"},{"key":"ref10","series-title":"Thromb Haemost","doi-asserted-by":"publisher","first-page":"652","DOI":"10.1160\/TH05-10-0653","article-title":"Dose- and time-dependent antiplatelet effects of aspirin","volume":"95","author":"Perneby","year":"2006"},{"key":"ref11","series-title":"Thromb Res","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1016\/S0049-3848(99)00126-7","article-title":"Optimization of an enzyme immunoassay for 11-dehydro-thromboxane B(2) in urine: comparison with GC-MS","volume":"96","author":"Perneby","year":"1999"},{"key":"ref12","series-title":"Proc Natl Acad Sci U S A","doi-asserted-by":"publisher","first-page":"5861","DOI":"10.1073\/pnas.83.16.5861","article-title":"11-Dehydrothromboxane B2: a quantitative index of thromboxane A2 formation in the human circulation","volume":"83","author":"Catella","year":"1986"},{"key":"ref13","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"1106","DOI":"10.1111\/j.1939-1676.2011.00781.x","article-title":"Platelet cyclooxygenase expression in normal dogs","volume":"25","author":"Thomason","year":"2011"},{"key":"ref14","series-title":"J Am Vet Med Assoc","doi-asserted-by":"publisher","first-page":"721","DOI":"10.2460\/javma.2000.217.721","article-title":"Physiologic characteristics and clinical importance of the cyclooxygenase isoforms in dogs and cats","volume":"217","author":"Jones","year":"2000"},{"key":"ref15","series-title":"Blood","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1182\/blood.V69.1.180.180","article-title":"Inhibition of thromboxane formation in vivo and ex vivo: implications for therapy with platelet inhibitory drugs","volume":"69","author":"Reilly","year":"1987"},{"key":"ref16","series-title":"J Am Anim Hosp Assoc","first-page":"23","article-title":"The effect of three different dosages of acetylsalicylic acid on canine platelet aggregation","volume":"24","author":"Rackear","year":"1988"},{"key":"ref17","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1111\/jvim.12022","article-title":"Cyclooxygenase expression and platelet function in healthy dogs receiving low-dose aspirin","volume":"27","author":"Dudley","year":"2013"},{"key":"ref18","series-title":"Vet Clin Pathol","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1111\/j.1939-165X.2007.tb00222.x","article-title":"Platelet function in dogs: breed differences and effect of acetylsalicylic acid administration","volume":"36","author":"Nielsen","year":"2007"},{"key":"ref19","series-title":"Eur Heart J","doi-asserted-by":"publisher","first-page":"1702","DOI":"10.1093\/eurheartj\/ehm226","article-title":"A comparison of six major platelet function tests to determine the prevalence of aspirin resistance in patients with stable coronary artery disease","volume":"28","author":"Lordkipanidz\u00e9","year":"2007"},{"key":"ref20","series-title":"Vet J","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1016\/j.tvjl.2009.05.028","article-title":"Measurement of platelet function in dogs using a novel impedance aggregometer","volume":"185","author":"Kalbantner","year":"2010"},{"key":"ref21","series-title":"Eur J Vasc Endovasc Surg","doi-asserted-by":"publisher","first-page":"456","DOI":"10.1016\/j.ejvs.2003.12.025","article-title":"Aspirin resistance in cardiovascular disease: a review","volume":"27","author":"Wong","year":"2004"},{"key":"ref22","series-title":"Lancet","doi-asserted-by":"publisher","first-page":"606","DOI":"10.1016\/S0140-6736(06)68040-9","article-title":"Aspirin resistance","volume":"367","author":"Hankey","year":"2006"},{"key":"ref23","series-title":"Nat Rev Drug Discov","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1038\/nrd985","article-title":"Scientific and therapeutic advances in antiplatelet therapy","volume":"2","author":"Bhatt","year":"2003"},{"key":"ref24","series-title":"Platelets","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1080\/09537100120104890","article-title":"Towards a definition of aspirin resistance: a typological approach","volume":"13","author":"Weber","year":"2002"},{"key":"ref25","series-title":"Vet Ther","first-page":"237","article-title":"Guidelines for safe and effective use of NSAIDs in dogs","volume":"6","author":"Lascelles","year":"2005"},{"key":"ref26","series-title":"Chrono-Log 700 manual","year":"2006"},{"key":"ref27","series-title":"J Thromb Haemost","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1111\/j.1538-7836.2008.02891.x","article-title":"Standardization of light transmittance aggregometry for monitoring antiplatelet therapy: an adjustment for platelet count is not necessary","volume":"6","author":"Linnemann","year":"2008"},{"key":"ref28","series-title":"Platelets","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1080\/09537100701326721","article-title":"To adjust or not to adjust the platelet count in light transmission aggregometry in patients receiving dual aspirin\/clopidogrel treatment","volume":"18","author":"van der Stelt","year":"2007"},{"key":"ref29","series-title":"J Clin Pathol","doi-asserted-by":"publisher","first-page":"747","DOI":"10.1136\/jcp.2004.022129","article-title":"Use of native or platelet count adjusted platelet rich plasma for platelet aggregation measurements","volume":"58","author":"Mani","year":"2005"},{"key":"ref30","series-title":"Haematologica","doi-asserted-by":"publisher","first-page":"694","DOI":"10.3324\/haematol.10999","article-title":"Platelet aggregation studies: autologous platelet-poor plasma inhibits platelet aggregation when added to platelet-rich plasma to normalize platelet count","volume":"92","author":"Cattaneo","year":"2007"},{"key":"ref31","series-title":"Multiplate Analyzer manual","year":"2012"},{"key":"ref32","series-title":"Haematologica","doi-asserted-by":"publisher","first-page":"2095","DOI":"10.3324\/haematol.2010.027102","article-title":"Inconsistency of different methods for assessing ex vivo platelet function: relevance for the detection of aspirin resistance","volume":"95","author":"Renda","year":"2010"},{"key":"ref33","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"472","DOI":"10.1111\/j.1939-1676.2007.tb02992.x","article-title":"Effects of 2 concentrations of sodium citrate on coagulation test results, von Willebrand factor concentration, and platelet function in dogs","volume":"21","author":"Morales","year":"2007"},{"key":"ref34","series-title":"Vet J","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/S1090-0233(02)00169-7","article-title":"Influence of platelet count, acetylsalicylic acid, von Willebrand's disease, coagulopathies, and haematocrit on results obtained using a platelet function analyser in dogs","volume":"165","author":"Mischke","year":"2003"},{"key":"ref35","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"652","DOI":"10.2460\/ajvr.2001.62.652","article-title":"Assessment of a point-of-care instrument for identification of primary hemostatic disorders in dogs","volume":"62","author":"Callan","year":"2001"},{"key":"ref36","series-title":"Thromb Res","doi-asserted-by":"publisher","first-page":"e132","DOI":"10.1016\/j.thromres.2009.11.005","article-title":"The risk of false results in the assessment of platelet function in the absence of antiplatelet medication: comparison of the PFA-100, multiplate electrical impedance aggregometry and verify now assays","volume":"125","author":"Can","year":"2010"},{"key":"ref37","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"1038","DOI":"10.2460\/ajvr.72.8.1038","article-title":"Evaluation of effects of low-dose aspirin administration on urinary thromboxane metabolites in healthy dogs","volume":"72","author":"Hoh","year":"2011"},{"key":"ref38","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"78","DOI":"10.2460\/ajvr.67.1.78","article-title":"Measurement of urinary 11-dehydro-thromboxane B2 excretion in dogs with gastric dilatation-volvulus","volume":"67","author":"Baltzer","year":"2006"},{"key":"ref39","series-title":"Neurosci Lett","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1016\/S0304-3940(01)02260-1","article-title":"Urinary 11-dehydro-thromboxane B(2) and coagulation activation markers measured within 24 h of human acute ischemic stroke","volume":"313","author":"McConnell","year":"2001"},{"key":"ref40","series-title":"Biometrics","doi-asserted-by":"publisher","first-page":"159","DOI":"10.2307\/2529310","article-title":"The measurement of observer agreement for categorical data","volume":"33","author":"Landis","year":"1977"},{"key":"ref41","series-title":"Thromb J","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1477-9560-2-1","article-title":"The antithrombotic profile of aspirin. Aspirin resistance, or simply failure?","volume":"2","author":"Altman","year":"2004"},{"key":"ref42","series-title":"Platelets","first-page":"519","article-title":"Clinical tests of platelet function","author":"Harrison","year":"2013"},{"key":"ref43","series-title":"Exp Toxicol Pathol","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1016\/j.etp.2012.07.003","article-title":"Optimization of Multiplate whole blood platelet aggregometry in the Beagle dog and Wi-star rat for ex vivo drug toxicity testing","volume":"65","author":"Defontis","year":"2013"},{"key":"ref44","series-title":"J Vet Emerg Crit Care (San Antonio)","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1111\/j.1476-4431.2011.00709.x","article-title":"Evaluation of platelet aggregometry in dogs using the Multiplate platelet analyzer: impact of anticoagulant choice and assay duration","volume":"22","author":"Marschner","year":"2012"},{"key":"ref45","series-title":"Int J Lab Hematol","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1111\/j.1751-553X.2010.01286.x","article-title":"The assessment of aspirin resistance by using light transmission and multiple electrode aggregometry","volume":"33","author":"Ulehlova","year":"2011"},{"key":"ref46","series-title":"JAm Vet Med Assoc","first-page":"1177","article-title":"Aspirin dosages for the cat","volume":"163","author":"Yeary","year":"1973"},{"key":"ref47","series-title":"J Vet Pharmacol Ther","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1111\/j.1365-2885.1993.tb00174.x","article-title":"Plasma salicylate concentrations in immature dogs following aspirin administration: comparison with adult dogs","volume":"16","author":"Waters","year":"1993"},{"key":"ref48","series-title":"Am J Vet Res","first-page":"1586","article-title":"Serum salicylate concentrations and endoscopic evaluation of the gastric mucosa in dogs after oral administration of aspirin-containing products","volume":"47","author":"Lipowitz","year":"1986"},{"key":"ref49","series-title":"J Am Vet Med Assoc","first-page":"65","article-title":"Clinical pharmacology of salicylates","volume":"176","author":"Davis","year":"1980"},{"key":"ref50","series-title":"J S Afr Vet Assoc","first-page":"191","article-title":"Pharmacokinetics of aspirin and its application in canine veterinary medicine","volume":"60","author":"Morton","year":"1989"},{"key":"ref51","series-title":"Clin Sci (Lond)","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1042\/cs0740491","article-title":"Inhibition of platelet aggregation and thromboxane production by low concentrations of aspirin in vitro","volume":"74","author":"Sils","year":"1988"},{"key":"ref52","series-title":"J Thromb Thrombolysis","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1007\/s11239-010-0489-x","article-title":"Platelet activation patterns in platelet size sub-populations: differential responses to aspirin in vitro","volume":"30","author":"Mangalpally","year":"2010"},{"key":"ref53","series-title":"Thromb Res","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1016\/0049-3848(95)93882-Z","article-title":"Evaluation of plasma 11-dehydro-thromboxane B2 as an indicator for thromboxane A2 synthesis in vivo in laboratory animals","volume":"77","author":"Kawamura","year":"1995"},{"key":"ref54","series-title":"J Clin Invest","doi-asserted-by":"publisher","first-page":"2469","DOI":"10.1172\/JCI116855","article-title":"Thromboxane-insensitive dog platelets have impaired activation of phospholipase C due to receptor-linked G protein dysfunction","volume":"92","author":"Johnson","year":"1993"},{"key":"ref55","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"1569","DOI":"10.2460\/ajvr.73.10.1569","article-title":"Effects of oral prednisone administration with or without ultralow-dose acetylsalicylic acid on coagulation parameters in healthy dogs","volume":"73","author":"O'Kell","year":"2012"},{"key":"ref56","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"1325","DOI":"10.2460\/ajvr.72.10.1325","article-title":"Independent and combined effects of prednisone and acetylsalicylic acid on thromboelastography variables in healthy dogs","volume":"72","author":"Flint","year":"2011"},{"key":"ref57","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"1294","DOI":"10.2460\/ajvr.71.11.1294","article-title":"Influence of treatment with ultralow-dose aspirin on platelet aggregation as measured by whole blood impedance aggregometry and platelet P-selectin expression in clinically normal dogs","volume":"71","author":"Sharpe","year":"2010"}],"container-title":["American Journal of Veterinary 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