{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,8]],"date-time":"2025-03-08T05:22:38Z","timestamp":1741411358468,"version":"3.38.0"},"reference-count":31,"publisher":"American Veterinary Medical Association (AVMA)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>OBJECTIVE<\/jats:bold> To evaluate the eosinophilic response in intestinal mucosa of horses with intestinal ischemia and reperfusion or with strangulation of the jejunum or colon.<\/jats:p>\n<jats:p><jats:bold>SAMPLE<\/jats:bold> Mucosal samples from horses with naturally occurring strangulation (n = 24 horses) or distention (n = 6) of the jejunum or colon (11), with experimentally induced ischemia and reperfusion of the jejunum (6) or colon (15), or that were euthanized for reasons other than gastrointestinal tract disease (13).<\/jats:p>\n<jats:p><jats:bold>PROCEDURES<\/jats:bold> Mucosal samples were collected and grouped by type of intestinal injury. Slides were stained with Luna eosinophil stain and histologically examined to determine eosinophil accumulation and distribution. Number of eosinophils per mm<jats:sup>2<\/jats:sup> of mucosa was calculated as a measure of eosinophil accumulation. Additionally, mucosa was categorized into 5 regions; the percentage of eosinophils in each of the 5 regions, relative to the total eosinophil count in all regions, was determined.<\/jats:p>\n<jats:p><jats:bold>RESULTS<\/jats:bold> Eosinophil migration toward and onto the luminal surface was evident in tissues after ischemia and reperfusion and after naturally occurring strangulating disease of the jejunum and colon, as indicated by a decrease in the number of eosinophils near the muscularis mucosa and an increase in the number of eosinophils on or near the luminal surface. Ischemia alone did not change eosinophil distribution in the jejunum or colon.<\/jats:p>\n<jats:p><jats:bold>CONCLUSIONS AND CLINICAL RELEVANCE<\/jats:bold> Eosinophils responded to mucosal damage evoked by ischemia and reperfusion by migration toward and onto the luminal surface. This migration could represent an important component of the inflammatory response to injury in equine gastrointestinal mucosa.<\/jats:p>","DOI":"10.2460\/ajvr.77.5.534","type":"journal-article","created":{"date-parts":[[2016,4,25]],"date-time":"2016-04-25T17:39:49Z","timestamp":1461605989000},"page":"534-539","source":"Crossref","is-referenced-by-count":4,"title":["Effects of ischemia and reperfusion on eosinophilic accumulation and distribution in mucosa of equine jejunum and colon"],"prefix":"10.2460","volume":"77","author":[{"given":"Anna K.","family":"R\u00f6tting","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David E.","family":"Freeman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter D.","family":"Constable","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo Ann 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Immunopathol","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1016\/S0165-2427(00)00222-1","article-title":"Cloning of equine chemokines eotaxin, monocyte chemoattractant protein (MCP)-1, MCP-2 and MCP-4, mRNA expression in tissues and induction by IL-4 in dermal fibroblasts","volume":"76","author":"Benarafa","year":"2000"},{"key":"ref11","series-title":"Am J Vet Res","first-page":"1153","article-title":"Histamine-induced adherence and migration of equine eosinophils","volume":"59","author":"Foster","year":"1998"},{"key":"ref12","series-title":"J Vet Pharmacol Ther","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1046\/j.1365-2885.2003.00453.x","article-title":"Substance P induces activation, adherence and migration of equine eosinophils","volume":"26","author":"Foster","year":"2003"},{"key":"ref13","series-title":"Inflamm Res","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1007\/s00011-004-1329-2","article-title":"The role of protein kinase C in regulating equine eosinophil adherence and superoxide production","volume":"54","author":"Sepulveda","year":"2005"},{"key":"ref14","series-title":"Am J Vet Res","first-page":"340","article-title":"Effect of ischemia and reperfusion on oxidative processes in the large colon and jejunum of horses","volume":"59","author":"Kooreman","year":"1998"},{"key":"ref15","series-title":"Am J Vet Res","first-page":"807","article-title":"Myeloperoxidase activity of the large intestine in an equine model of acute colitis","volume":"60","author":"McConnico","year":"1999"},{"key":"ref16","series-title":"Am J Vet Res","first-page":"1454","article-title":"Neutrophil accumulation in the large colon of horses during low-flow ischemia and reperfusion","volume":"55","author":"Moore","year":"1994"},{"key":"ref17","series-title":"Compend Contin Educ Pract Vet","first-page":"342","article-title":"Idiopathic eosinophilic enteritis in a 10-week-old colt","volume":"24","author":"Stanar","year":"2002"},{"key":"ref18","series-title":"Equine Vet J Suppl","first-page":"86","article-title":"Segmental eosinophilic colitis: a review of 22 cases","author":"Edwards","year":"2000"},{"key":"ref19","series-title":"Vet Surg","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1053\/jvet.2000.7543","article-title":"Idiopathic focal eosinophilic enteritis associated with small intestinal obstruction in 6 horses","volume":"29","author":"Southwood","year":"2000"},{"key":"ref20","series-title":"Vet Pathol","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1177\/030098589603300215","article-title":"Eosinophilic colitis and hepatitis in a horse with colonic intramucosal ciliated protozoa","volume":"33","author":"French","year":"1996"},{"key":"ref21","series-title":"Tijdschr Diergeneeskd","first-page":"680","article-title":"Een paard met een chronische eosinofiele enteritis","volume":"120","author":"van der 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Res","doi-asserted-by":"publisher","first-page":"53","DOI":"10.2460\/ajvr.73.1.53","article-title":"Effects of ischemia and reperfusion on production or nitrotyrosine, activation of eosinophils, and apoptosis in the large colonic mucosa of horses","volume":"73","author":"Grosche","year":"2012"},{"key":"ref26","series-title":"Equine Vet J","first-page":"329","article-title":"Post operative neutrophilic inflammation in equine small intestine after manipulation and ischemia","volume":"37","author":"Little","year":"2005"},{"key":"ref27","series-title":"Histopathologic methods and color atlas of special stains and tissue artifacts","first-page":"255","author":"Luna","year":"1992"},{"key":"ref28","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"1205","DOI":"10.2460\/ajvr.2003.64.1205","article-title":"Effects of acetylcysteine and migration of resident eosinophils in an in vitro model of mucosal injury and restitution in equine right dorsal 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