{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:37:57Z","timestamp":1760985477515,"version":"3.38.0"},"reference-count":41,"publisher":"American Veterinary Medical Association (AVMA)","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2016,8]]},"abstract":"Abstract<\/jats:title>\nOBJECTIVE<\/jats:bold> To assess platelet closure time (CT), mean platelet component (MPC) concentration, and platelet component distribution width (PCDW) in dogs with subclinical chronic valvular heart disease.<\/jats:p>\nANIMALS<\/jats:bold> 89 Cavalier King Charles Spaniels (CKCSs) and 39 control dogs (not CKCSs).<\/jats:p>\nPROCEDURES<\/jats:bold> Platelet count, MPC concentration, PCDW, and Hct were measured by use of a hematology analyzer, and CT was measured by use of a platelet function analyzer. Murmur grade and echocardiographic variables (mitral valve regurgitant jet size relative to left atrial area, left atrial-to-aortic diameter ratio, and left ventricular internal dimensions) were recorded. Associations between explanatory variables (sex, age, murmur grade, echocardiographic variables, platelet count, and Hct) and outcomes (CT, MPC concentration, and PCDW) were examined by use of multivariate regression models.<\/jats:p>\nRESULTS<\/jats:bold> A model with 5 variables best explained variation in CT (R2<\/jats:sup>, 0.74), with > 60% of the variance of CT explained by mitral valve regurgitant jet size. The model of best fit to explain variation in MPC concentration included only platelet count (R2<\/jats:sup>, 0.24). The model of best fit to explain variation in PCDW included platelet count and sex (R<\/jats:italic>2<\/jats:sup>, 0.25).<\/jats:p>\nCONCLUSIONS AND CLINICAL RELEVANCE<\/jats:bold> In this study, a significant effect of mitral valve regurgitant jet size on CT was consistent with platelet dysfunction. However, platelet activation, as assessed on the basis of the MPC concentration and PCDW, was not a feature of subclinical chronic valvular heart disease in CKCSs.<\/jats:p>","DOI":"10.2460\/ajvr.77.8.860","type":"journal-article","created":{"date-parts":[[2016,7,27]],"date-time":"2016-07-27T17:48:34Z","timestamp":1469641714000},"page":"860-868","source":"Crossref","is-referenced-by-count":5,"title":["Platelet function and activation in Cavalier King Charles Spaniels with subclinical chronic valvular heart disease"],"prefix":"10.2460","volume":"77","author":[{"given":"Linda J.","family":"Tong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giselle L.","family":"Hosgood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne T.","family":"French","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Irwin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert E.","family":"Shiel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"Textbook of canine and feline cardiology principles and clinical practice.","first-page":"536","article-title":"Acquired valvular heart diseases of dogs and cats","author":"Sisson","year":"1999"},{"key":"ref2","series-title":"Vet Rec","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1136\/vr.144.12.315","article-title":"Echocardiographic mitral valve prolapse in Cavalier King Charles Spaniels: epidemiology and prognostic significance for regurgitation","volume":"144","author":"Pedersen","year":"1999"},{"key":"ref3","series-title":"J Lab Clin Med","first-page":"462","article-title":"Morphological, biochemical, and functional changes in human platelets subjected to shear 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