{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T19:03:10Z","timestamp":1790535790590,"version":"4.1.0"},"reference-count":25,"publisher":"American Veterinary Medical Association (AVMA)","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>OBJECTIVE<\/jats:bold> To determine the prevalence of naturally occurring anti-dog erythrocyte antigen (DEA) 7 antibodies in DEA 7\u2013negative dogs from Spain and Italy.<\/jats:p>\n<jats:p><jats:bold>ANIMALS<\/jats:bold> 252 DEA 7\u2013negative dogs from a population of 312 dogs that were previously tested for DEA 1, DEA 4, and DEA 7.<\/jats:p>\n<jats:p><jats:bold>PROCEDURES<\/jats:bold> A plasma sample was obtained from each dog and evaluated for anti-DEA 7 antibodies by the use of gel column agglutination. Each plasma sample underwent major crossmatching with RBCs from DEA 7-positive dogs. Samples that resulted in agglutination were then crossmatched with RBCs from DEA 1-negative, DEA 4-positive, and DEA 7\u2013negative dogs to confirm the presence of anti-DEA 7 antibodies. Results were then used to calculate the risk for a delayed transfusion reaction in a DEA 7\u2013negative dog with anti-DEA 7 antibodies after a transfusion with blood that was not crossmatched or typed for DEA 7.<\/jats:p>\n<jats:p><jats:bold>RESULTS<\/jats:bold> 96 of 252 (38.1%) plasma samples contained anti-DEA 7 antibodies. A DEA 7\u2013negative dog with anti-DEA 7 antibodies had a 5.9% chance of developing a delayed hemolytic reaction after transfusion with blood not crossmatched or typed for DEA 7.<\/jats:p>\n<jats:p><jats:bold>CONCLUSIONS AND CLINICAL RELEVANCE<\/jats:bold> Results indicated that canine blood used for transfusion should be crossmatched with the blood or plasma of the intended recipient prior to transfusion to minimize the likelihood that the recipient will develop a hemolytic reaction associated with anti-DEA 7 antibodies. Ideal canine blood donors should be negative for both DEA 1 and DEA 7.<\/jats:p>","DOI":"10.2460\/ajvr.77.8.877","type":"journal-article","created":{"date-parts":[[2016,7,27]],"date-time":"2016-07-27T17:48:34Z","timestamp":1469641714000},"page":"877-881","source":"Crossref","is-referenced-by-count":25,"title":["Prevalence of naturally occurring antibodies against dog erythrocyte antigen 7 in a population of dog erythrocyte antigen 7\u2013negative dogs from Spain and Italy"],"prefix":"10.2460","volume":"77","author":[{"given":"Eva","family":"Spada","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Proverbio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis Miguel","family":"Vi\u00f1als Fl\u00f3rez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"del Rosario Perlado Chamizo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blanca","family":"Serra y G\u00f3mez de la Serna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Perego","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana","family":"Baggiani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"J Vet Emerg Crit Care (San Antonio)","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1111\/j.1476-4431.2009.00387.x","article-title":"Increasing patient safety in veterinary transfusion medicine: an overview of pretransfusion testing","volume":"19","author":"Tocci","year":"2009"},{"key":"ref2","series-title":"Modern blood banking and transfusion practices","first-page":"45","article-title":"Fundamentals of immunology","author":"Caruccio","year":"2012"},{"key":"ref3","series-title":"J Am Vet Med Assoc","first-page":"1358","article-title":"An acute hemolytic transfusion reaction caused by dog erythrocyte antigen 1.1 incompatibility in a previously sensitized dog","volume":"206","author":"Giger","year":"1995"},{"key":"ref4","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1111\/j.1939-1676.1995.tb01080.x","article-title":"Hemolytic transfusion reactions in a dog with an alloantibody to a common antigen","volume":"9","author":"Callan","year":"1995"},{"key":"ref5","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1111\/j.1939-1676.2007.tb02961.x","article-title":"Canine Dal blood type: a red cell antigen lacking in some Dalmatians","volume":"21","author":"Blais","year":"2007"},{"key":"ref6","series-title":"Vet Clin Pathol","doi-asserted-by":"publisher","first-page":"306","DOI":"10.1111\/j.1939-165X.2010.00249.x","article-title":"Dog erythrocyte antigens 1.1, 1.2, 3, 4, 7, and Dal blood typing and crossmatching by gel column technique","volume":"39","author":"Kessler","year":"2010"},{"key":"ref7","series-title":"Vox Sang","doi-asserted-by":"publisher","first-page":"542","DOI":"10.1111\/j.1423-0410.1971.tb00466.x","article-title":"Tr: a canine red cell antigen related to the A-antigen of human red cells","volume":"20","author":"Bowdler","year":"1971"},{"key":"ref8","series-title":"Tissue Antigens","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1111\/tan.1973.3.3.145","article-title":"Joint report of 1st International Workshop on Canine Immunogenetics","volume":"3","author":"Vriesendorp","year":"1973"},{"key":"ref9","series-title":"Anim Blood Groups Biochem Genet","first-page":"13","article-title":"Canine blood groups. 2. 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