{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T18:08:49Z","timestamp":1790618929104,"version":"4.1.0"},"reference-count":35,"publisher":"American Veterinary Medical Association (AVMA)","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>OBJCTIVE<\/jats:bold> To investigate the effects of dietary lipid overload on bile acid metabolism and gallbladder motility in healthy dogs.<\/jats:p>\n<jats:p><jats:bold>ANIMALS<\/jats:bold> 7 healthy Beagles.<\/jats:p>\n<jats:p><jats:bold>PROCEDURES<\/jats:bold> In a crossover study, dogs were fed a high-fat\u2013high-cholesterol diet (HFCD) or a low-fat diet (LFD) for a period of 2 weeks. After a 4-month washout period, dogs were fed the other diet for 2 weeks. Before and at the end of each feeding period, the concentrations of each of the gallbladder bile acids, cholecystokinin (CCK)-induced gallbladder motility, and bile acid metabolism\u2013related hepatic gene expression were examined in all dogs.<\/jats:p>\n<jats:p><jats:bold>RESULTS<\/jats:bold> The HFCD significantly increased plasma total cholesterol concentrations. The HFCD also increased the concentration of taurochenodeoxycholic acid and decreased the concentration of taurocholic acid in bile and reduced gallbladder contractility, whereas the LFD significantly decreased the concentration of taurodeoxycholic acid in bile. Gene expression analysis revealed significant elevation of cholesterol 7\u03b1-hydroxylase mRNA expression after feeding the HFCD for 2 weeks, but the expression of other genes was unchanged.<\/jats:p>\n<jats:p><jats:bold>CONCLUSIONS AND CLINICAL RELEVANCE<\/jats:bold> Feeding the HFCD and LFD for 2 weeks induced changes in gallbladder bile acid composition and gallbladder motility in dogs. In particular, feeding the HFCD caused an increase in plasma total cholesterol concentration, an increase of hydrophobic bile acid concentration in bile, and a decrease in gallbladder sensitivity to CCK. These results suggested that similar bile acid compositional changes and gallbladder hypomotility might be evident in dogs with hyperlipidemia.<\/jats:p>","DOI":"10.2460\/ajvr.78.12.1406","type":"journal-article","created":{"date-parts":[[2017,11,28]],"date-time":"2017-11-28T14:29:50Z","timestamp":1511879390000},"page":"1406-1413","source":"Crossref","is-referenced-by-count":29,"title":["Effect of a high-fat\u2013high-cholesterol diet on gallbladder bile acid composition and gallbladder motility in 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mucocele","volume":"41","author":"Besso","year":"2000"},{"key":"ref2","series-title":"J Am Vet Med Assoc","doi-asserted-by":"publisher","first-page":"1615","DOI":"10.2460\/javma.2004.224.1615","article-title":"Gallbladder mucocele in dogs: 30 cases (2000\u20132002)","volume":"224","author":"Pike","year":"2004"},{"key":"ref3","series-title":"J Am Vet Med Assoc","doi-asserted-by":"publisher","first-page":"79","DOI":"10.2460\/javma.231.1.79","article-title":"Gallbladder disease in Shetland Sheepdogs: 38 cases (1995\u20132005)","volume":"231","author":"Aguirre","year":"2007"},{"key":"ref4","series-title":"J S Afr Vet Assoc","first-page":"1318","article-title":"Gallbladder mucocoele: a review","volume":"86","author":"Smalle","year":"2015"},{"key":"ref5","series-title":"J Vet Med Sci","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1292\/jvms.56.299","article-title":"Changes in bile acid composition of serum and gallbladder bile in bile duct ligated dogs","volume":"56","author":"Washizu","year":"1994"},{"key":"ref6","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"223","DOI":"10.2460\/ajvr.78.2.223","article-title":"Bile acid composition of gallbladder contents in dogs with gallbladder mucocele and biliary sludge","volume":"78","author":"Kakimoto","year":"2017"},{"key":"ref7","series-title":"Vet Radiol Ultrasound","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1111\/j.1740-8261.2011.01868.x","article-title":"Decreased gallbladder emptying in dogs with biliary sludge or gallbladder mucocele","volume":"53","author":"Tsukagoshi","year":"2012"},{"key":"ref8","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1111\/jvim.14612","article-title":"Increased leptin and leptin receptor expression in dogs with gallbladder mucocele","volume":"31","author":"Lee","year":"2017"},{"key":"ref9","series-title":"Gut","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1136\/gut.52.1.109","article-title":"Gall bladder dysmotility: a risk factor for gall stone formation in hypertriglyceridaemia and reversal on triglyceride lowering therapy by bezafibrate and fish oil","volume":"52","author":"Jonkers","year":"2003"},{"key":"ref10","series-title":"Hepatology","doi-asserted-by":"publisher","first-page":"2112","DOI":"10.1002\/hep.22204","article-title":"Coordinate regulation of gallbladder motor function in the gut-liver axis","volume":"47","author":"Portincasa","year":"2008"},{"key":"ref11","series-title":"Eur J Clin Invest","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1111\/eci.12580","article-title":"The cholecystokinin-1 receptor antagonist devazepide increases cholesterol cholelithogenesis in mice","volume":"46","author":"Wang","year":"2016"},{"key":"ref12","series-title":"PLoS One","doi-asserted-by":"publisher","first-page":"e0138988","DOI":"10.1371\/journal.pone.0138988","article-title":"Excess secretion of gelforming mucins and associated innate defense proteins with defective mucin un-packaging underpin gallbladder mucocele formation in dogs","volume":"10","author":"Kesimer","year":"2015"},{"key":"ref13","series-title":"J Small Anim Pract","doi-asserted-by":"publisher","first-page":"630","DOI":"10.1111\/j.1748-5827.2009.00811.x","article-title":"Gall bladder mucoceles and their association with endocrinopathies in dogs: a retrospective case-control study","volume":"50","author":"Mesich","year":"2009"},{"key":"ref14","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"1464","DOI":"10.1111\/jvim.13649","article-title":"Association of gallbladder mucocele histologic diagnosis with selected drug use in dogs: a matched case-control study","volume":"29","author":"Gookin","year":"2015"},{"key":"ref15","series-title":"Vet J","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1016\/j.tvjl.2013.10.019","article-title":"The association between gall bladder mucoceles and hyperlipidaemia in dogs: a retrospective case control study","volume":"199","author":"Kutsunai","year":"2014"},{"key":"ref16","series-title":"Vet J","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1016\/j.tvjl.2014.02.024","article-title":"The association between hyperlipidemia and gall bladder mucoceles in dogs","volume":"200","author":"Xenoulis","year":"2014"},{"key":"ref17","series-title":"World J Gastroenterol","doi-asserted-by":"publisher","first-page":"923","DOI":"10.3748\/wjg.v18.i9.923","article-title":"High-fat-induced intestinal permeability dysfunction associated with altered fecal bile acids","volume":"18","author":"Stenman","year":"2012"},{"key":"ref18","series-title":"Nat Rev Drug Discov","doi-asserted-by":"publisher","first-page":"678","DOI":"10.1038\/nrd2619","article-title":"Targeting bileacid signalling for metabolic diseases","volume":"7","author":"Thomas","year":"2008"},{"key":"ref19","series-title":"J Endocrinol","first-page":"233","article-title":"Liver X receptor in cholesterol metabolism","volume":"204","author":"Zhao","year":"2010"},{"key":"ref20","series-title":"J Clin Invest","doi-asserted-by":"publisher","first-page":"1408","DOI":"10.1172\/JCI21025","article-title":"Bile acids lower triglyceride levels via a pathway involving FXR, SHP, and SREBP-1c","volume":"113","author":"Watanabe","year":"2004"},{"key":"ref21","series-title":"Br J Nutr","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1017\/S0007114514003717","article-title":"Effect of a high-fat diet on the hepatic expression of nuclear receptors and their target genes: relevance to drug disposition","volume":"113","author":"Ghoneim","year":"2015"},{"key":"ref22","series-title":"Vet Radiol Ultrasound","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1111\/vru.12224","article-title":"Feasibility of percutaneous contrast ultrasound-guided cholecystography in dogs","volume":"56","author":"Ji","year":"2015"},{"key":"ref23","series-title":"Chem Lett","first-page":"1443","article-title":"A new analytical method of individual bile acids using high performance liquid chromatography and immobilized 3\u03b1-hydroxysteroid dehydrogenase in column form","volume":"12","author":"Okuyama","year":"1979"},{"key":"ref24","series-title":"J Pharmacol Toxicol Methods","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1016\/1056-8719(92)90029-Z","article-title":"Real-time ultrasonography as a noninvasive tool for the examination of canine gallbladder emptying: a validation study","volume":"27","author":"Jonderko","year":"1992"},{"key":"ref25","series-title":"Acta Cir Bras","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1590\/S0102-86502014000400004","article-title":"Effects of cholecystectomy on the changes of motility of Beagle dogs' sphincter of Oddi","volume":"29","author":"Li","year":"2014"},{"key":"ref26","series-title":"Am J Physiol","first-page":"G665","article-title":"Contrasting cholinergic dependence of pancreatic and gallbladder responses to cholecystokinin","volume":"250","author":"Strah","year":"1986"},{"key":"ref27","series-title":"Vet Immunol Immunopathol","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1016\/j.vetimm.2007.01.011","article-title":"Development and application of multiple internal reference (housekeeper) gene assays for accurate normalisation of canine gene expression studies","volume":"117","author":"Peters","year":"2007"},{"key":"ref28","series-title":"Glob Adv Health Med","doi-asserted-by":"publisher","first-page":"58","DOI":"10.7453\/gahmj.2014.017","article-title":"The unexpected uses of urso- and tauroursodeoxycholic acid in the treatment of non-liver diseases","volume":"3","author":"Vang","year":"2014"},{"key":"ref29","series-title":"Neurogastroenterol Motil","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1111\/nmo.12094","article-title":"Roles of cholesterol and bile salts in the pathogenesis of gallbladder hypomotility and inflammation: cholecystitis is not caused by cystic duct obstruction","volume":"25","author":"Behar","year":"2013"},{"key":"ref30","series-title":"Lab Invest","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1038\/labinvest.2014.140","article-title":"Cholesterol gallstone disease: focusing on the role of gallbladder","volume":"95","author":"Chen","year":"2015"},{"key":"ref31","series-title":"Prog Liver Dis","first-page":"165","article-title":"Cholesterol gallstone formation. 2. Pathobiology and pathomechanics","volume":"10","author":"Lamont","year":"1992"},{"key":"ref32","series-title":"Am J Physiol Gastrointest Liver Physiol","doi-asserted-by":"publisher","first-page":"G490","DOI":"10.1152\/ajpgi.2001.281.2.G490","article-title":"Abnormalities of gallbladder muscle associated with acute inflammation in guinea pigs","volume":"281","author":"Xiao","year":"2001"},{"key":"ref33","series-title":"J Physiol","doi-asserted-by":"publisher","first-page":"3295","DOI":"10.1113\/jphysiol.2010.192146","article-title":"Hydrophobic bile salts inhibit gallbladder smooth muscle function via stimulation of GPBAR1 receptors and activation of KATP channels","volume":"588","author":"Lavoie","year":"2010"},{"key":"ref34","series-title":"Biochem J","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1042\/bj3320257","article-title":"Bile salts stimulate mucin secretion by cultured dog gallbladder epithelial cells independent of their detergent effect","volume":"332","author":"Klinkspoor","year":"1998"},{"key":"ref35","series-title":"Cell","doi-asserted-by":"publisher","first-page":"693","DOI":"10.1016\/S0092-8674(00)81432-4","article-title":"Cholesterol and bile acid metabolism are impaired in mice lacking the nuclear oxysterol receptor LXR alpha","volume":"93","author":"Peet","year":"1998"}],"container-title":["American Journal of Veterinary Research"],"original-title":[],"link":[{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/ajvr\/78\/12\/ajvr.78.12.1406.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/ajvr\/78\/12\/ajvr.78.12.1406.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/ajvr\/78\/12\/ajvr.78.12.1406.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T12:40:07Z","timestamp":1741351207000},"score":1,"resource":{"primary":{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/ajvr\/78\/12\/ajvr.78.12.1406.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12]]},"references-count":35,"journal-issue":{"issue":"12"},"URL":"https:\/\/doi.org\/10.2460\/ajvr.78.12.1406","relation":{},"ISSN":["0002-9645"],"issn-type":[{"value":"0002-9645","type":"print"}],"subject":[],"published":{"date-parts":[[2017,12]]}}}