{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T08:52:57Z","timestamp":1770972777104,"version":"3.50.1"},"reference-count":20,"publisher":"American Veterinary Medical Association (AVMA)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>OBJECTIVE<\/jats:bold> To evaluate a hypervariable octameric oligonucleotide fingerprints (HOOF-Prints) assay for identification of and discrimination between wild-type and vaccine strains of <jats:italic>Brucella melitensis.<\/jats:italic><\/jats:p>\n<jats:p><jats:bold>SAMPLE<\/jats:bold> <jats:italic>Brucella melitensis<\/jats:italic> vaccine strain M5 and wild-type strain M43.<\/jats:p>\n<jats:p><jats:bold>PROCEDURES<\/jats:bold> 8 pairs of primers (alterable, octameric nucleotides) were designed on the basis of a biological analysis of 8 flanking sequences in the DNA of <jats:italic>B melitensis.<\/jats:italic> The HOOF-Prints technique was used to identify wild-type and vaccine strains of <jats:italic>B melitensis.<\/jats:italic> Phylogenetic analysis of short, polymorphic fragments of DNA from <jats:italic>B melitensis<\/jats:italic> strains M5 and M43 was performed.<\/jats:p>\n<jats:p><jats:bold>RESULTS<\/jats:bold> Variable-number tandem repeat DNA segments of <jats:italic>B melitensis<\/jats:italic> vaccine strain M5 and wild-type strain M43 were successfully amplified by means of PCR assay. All target gene fragments ranged in size from 100 to 300 bp. Separate phylogenetic analysis of each <jats:italic>Brucella<\/jats:italic> strain revealed considerable differences between the vaccine and wild-type strains.<\/jats:p>\n<jats:p><jats:bold>CONCLUSIONS AND CLINICAL RELEVANCE<\/jats:bold> The results of this study suggested the HOOF-Prints assay may be useful for discriminating vaccine strains of <jats:italic>B melitensis<\/jats:italic> from wild-type strains. This ability could allow discrimination between animals that are seropositive because of vaccination against <jats:italic>B melitensis<\/jats:italic> and those that are seropositive because of <jats:italic>B melitensis<\/jats:italic> infection and could decrease the likelihood of importing Brucella-infected animals.<\/jats:p>","DOI":"10.2460\/ajvr.78.4.495","type":"journal-article","created":{"date-parts":[[2017,3,27]],"date-time":"2017-03-27T19:29:47Z","timestamp":1490642987000},"page":"495-499","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of a hypervariable octameric oligonucleotide fingerprints assay for identification of and discrimination between wild-type and vaccine strains of Brucella 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