{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T02:58:02Z","timestamp":1792292282871,"version":"4.4.0"},"reference-count":43,"publisher":"American Veterinary Medical Association (AVMA)","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>OBJECTIVE<\/jats:bold> To determine whether major histocompatability complex (MHC) class II expression in equine mesenchymal stem cells (MSCs) changes with exposure to a proinflammatory environment reflective of an inflamed joint.<\/jats:p>\n<jats:p><jats:bold>SAMPLE<\/jats:bold> Cryopreserved bone marrow-derived MSCs from 12 horses and cartilage and synovium samples from 1 horse euthanized for reasons other than lameness.<\/jats:p>\n<jats:p><jats:bold>PROCEDURES<\/jats:bold> In part 1 of a 3-part study, the suitability of a quantitative reverse transcriptase PCR (qRT-PCR) assay for measurement of MHC class II expression in MSCs following stimulation with interferon (IFN)-\u03b3 was assessed. In part 2, synoviocyte-cartilage cocultures were or were not stimulated with interleukin (IL)-1\u03b2 (10 ng\/mL) to generate conditioned media that did and did not (control) mimic an inflamed joint environment. In part 3, a qRT-PCR assay was used to measure MSC MHC class II expression after 96 hours of incubation with 1 of 6 treatments (control-conditioned medium, IL-1\u03b2-conditioned medium, and MSC medium alone [untreated control] or with IL-1\u03b2 [10 ng\/mL], tumor necrosis factor-\u03b1 [10 ng\/mL], or IFN-\u03b3 [100 ng\/mL]).<\/jats:p>\n<jats:p><jats:bold>RESULTS<\/jats:bold> The qRT-PCR assay accurately measured MHC class II expression. Compared with MHC class II expression for MSCs exposed to the untreated control medium, that for MSCs exposed to IL-1\u03b2 was decreased, whereas that for MSCs exposed to IFN-\u03b3 was increased. Neither the control-conditioned nor tumor necrosis factor-\u03b1 medium altered MHC class II expression.<\/jats:p>\n<jats:p><jats:bold>CONCLUSIONS AND CLINICAL RELEVANCE<\/jats:bold> Results indicated that MSC exposure to proinflammatory cytokine IL-1\u03b2 decreased MHC class II expression and antigenicity. Treatment of inflamed joints with allogeneic MSCs might not be contraindicated, but further investigation is warranted.<\/jats:p>","DOI":"10.2460\/ajvr.78.7.867","type":"journal-article","created":{"date-parts":[[2017,6,26]],"date-time":"2017-06-26T14:33:27Z","timestamp":1498487607000},"page":"867-875","source":"Crossref","is-referenced-by-count":22,"title":["Antigenicity of mesenchymal stem cells in an inflamed joint environment"],"prefix":"10.2460","volume":"78","author":[{"given":"Jacqueline A.","family":"Hill","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer M.","family":"Cassano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret B.","family":"Goodale","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa 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