{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:32:37Z","timestamp":1761744757140,"version":"3.38.0"},"reference-count":39,"publisher":"American Veterinary Medical Association (AVMA)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>OBJECTIVE<\/jats:bold> To use proteomic analysis to determine the protein constituents of synovial fluid samples from the stifle joints of dogs with and without osteoarthritis secondary to cranial cruciate ligament rupture (CCLR).<\/jats:p>\n<jats:p><jats:bold>ANIMALS<\/jats:bold> 12 dogs with clinically normal stifle joints (controls) and 16 dogs with osteoarthritis secondary to CCLR.<\/jats:p>\n<jats:p><jats:bold>PROCEDURES<\/jats:bold> A synovial fluid sample was obtained from all dogs. Synovial fluid total protein concentration was determined by the Bradford assay. Proteins were separated by use of a 1-D SDS-PAGE to detect protein bands that differed between dogs with and without osteoarthritis. Those protein bands then underwent trypsin digestion and were analyzed by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry, the results of which were compared with a curated protein sequence database for protein identification. One of the most frequently identified proteins, apoprotein (apo) A-I, was then quantified in all synovial fluid samples by use of a competitive-inhibition ELISA. Results were compared between dogs with and without osteoarthritis.<\/jats:p>\n<jats:p><jats:bold>RESULTS<\/jats:bold> Median synovial fluid total protein and apo A-I concentrations for dogs with osteoarthritis were significantly greater than those for control dogs. The most abundant proteins identified in the synovial fluid were albumin and apo A-I.<\/jats:p>\n<jats:p><jats:bold>CONCLUSIONS AND CLINICAL RELEVANCE<\/jats:bold> Results indicated that quantification of synovial fluid total protein and apo A-I concentrations might facilitate diagnosis of osteoarthritis secondary to CCLR in dogs. Further research and validation of synovial fluid apo A-I concentration as a biomarker for osteoarthritis in dogs are necessary before it can be recommended for clinical use.<\/jats:p>","DOI":"10.2460\/ajvr.79.4.397","type":"journal-article","created":{"date-parts":[[2018,3,27]],"date-time":"2018-03-27T15:11:02Z","timestamp":1522163462000},"page":"397-403","source":"Crossref","is-referenced-by-count":6,"title":["Use of proteomic analysis to determine the protein constituents of synovial fluid samples from the stifle joints of dogs with and without osteoarthritis secondary to cranial cruciate ligament rupture"],"prefix":"10.2460","volume":"79","author":[{"given":"Muhammad","family":"Shahid","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Manchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leo","family":"Brunnberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jens","family":"Raila","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"J Knee Surg","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1055\/s-0031-1297361","article-title":"Using animal models in osteoarthritis biomarker research","volume":"24","author":"Garner","year":"2011"},{"key":"ref2","series-title":"Nat Rev Drug 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