{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T20:28:28Z","timestamp":1775075308313,"version":"3.50.1"},"reference-count":17,"publisher":"American Veterinary Medical Association (AVMA)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>OBJECTIVE<\/jats:bold> To determine whether stored (cooled or frozen-thawed) jejunal segments can be used to obtain dependable leak pressure data after enterotomy closure.<\/jats:p>\n<jats:p><jats:bold>SAMPLE<\/jats:bold> 36 jejunal segments from 3 juvenile pigs.<\/jats:p>\n<jats:p><jats:bold>PROCEDURES<\/jats:bold> Jejunal segments were harvested from euthanized pigs and assigned to 1 of 3 treatment groups (n = 12 segments\/group) as follows: fresh (used within 4 hours after collection), cooled (stored overnight at 5\u00b0C before use), and frozen-thawed (frozen at \u221212\u00b0C for 8 days and thawed at room temperature [23\u00b0C] for 1 hour before use). Jejunal segments were suspended and 2-cm enterotomy incisions were made on the antimesenteric border. Enterotomies were closed with a simple continuous suture pattern. Lactated Ringer solution was infused into each segment until failure at the suture line was detected. Leak pressure was measured by use of a digital transducer.<\/jats:p>\n<jats:p><jats:bold>RESULTS<\/jats:bold> Mean \u00b1 SD leak pressure for fresh, cooled, and frozen-thawed segments was 68.3 \u00b1 23.7 mm Hg, 55.3 \u00b1 28.1 mm Hg, and 14.4 \u00b1 14.8 mm Hg, respectively. Overall, there were no significant differences in mean leak pressure among pigs, but a significant difference in mean leak pressure was detected among treatment groups. Mean leak pressure was significantly lower for frozen-thawed segments than for fresh or cooled segments, but mean leak pressure did not differ significantly between fresh and cooled segments.<\/jats:p>\n<jats:p><jats:bold>CONCLUSIONS AND CLINICAL RELEVANCE<\/jats:bold> Fresh porcine jejunal segments or segments cooled overnight may be used for determining intestinal leak pressure, but frozen-thawed segments should not be used.<\/jats:p>","DOI":"10.2460\/ajvr.79.5.576","type":"journal-article","created":{"date-parts":[[2018,4,24]],"date-time":"2018-04-24T16:08:48Z","timestamp":1524586128000},"page":"576-580","source":"Crossref","is-referenced-by-count":22,"title":["Comparison of enterotomy leak pressure among fresh, cooled, and frozen-thawed porcine jejunal segments"],"prefix":"10.2460","volume":"79","author":[{"given":"Kimberly A.","family":"Aeschlimann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. A.","family":"Mann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John R.","family":"Middleton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca C.","family":"Belter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"J Am Anim Hosp Assoc","first-page":"70","article-title":"Prevalence of small intestinal dehiscence and associated clinical factors: a retrospective study of 121 dogs","volume":"28","author":"Allen","year":"1992"},{"key":"ref2","series-title":"J Am Vet Med Assoc","doi-asserted-by":"publisher","first-page":"486","DOI":"10.2460\/javma.238.4.486","article-title":"Identification of risk factors for septic peritonitis and failure to survive following gastrointestinal surgery in 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