{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,4]],"date-time":"2024-08-04T14:42:02Z","timestamp":1722782522612},"reference-count":44,"publisher":"American Veterinary Medical Association (AVMA)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>OBJECTIVE<\/jats:bold> To evaluate the lipidomic profile of surfactant obtained from horses with asthma at various clinical stages and to compare results with findings for healthy horses exposed to the same conditions.<\/jats:p>\n<jats:p><jats:bold>SAMPLE<\/jats:bold> Surfactant samples obtained from 6 horses with severe asthma and 7 healthy horses.<\/jats:p>\n<jats:p><jats:bold>PROCEDURES<\/jats:bold> Clinical evaluation of horses and surfactant analysis were performed. Samples obtained from horses with severe asthma and healthy horses before (baseline), during, and after exposure to hay were analyzed. Crude surfactant pellets were dried prior to dissolution in a solution of isopropanol:methanol:chloroform (4:2:1) containing 7.5mM ammonium acetate. Shotgun lipidomics were performed by use of high-resolution data acquisition on an ion-trap mass spectrometer. Findings were analyzed by use of an ANOVA with a Tukey-Kramer post hoc test.<\/jats:p>\n<jats:p><jats:bold>RESULTS<\/jats:bold> Results of lipidomic analysis were evaluated to detect significant differences between groups of horses and among exposure statuses within groups of horses. Significantly increased amounts of cyclic phosphatidic acid (cPA) and diacylglycerol (DAG) were detected in surfactant from severely asthmatic horses during exposure to hay, compared with baseline and postexposure concentrations. Concentrations of cPA and DAG did not change significantly in healthy horses regardless of exposure status.<\/jats:p>\n<jats:p><jats:bold>CONCLUSIONS AND CLINICAL RELEVANCE<\/jats:bold> cPA 16:0 and DAG 36:2 were 2 novel lipid mediators identified in surfactant obtained from asthmatic horses with clinical disease. These molecules were likely biomarkers of sustained inflammation. Further studies are needed to evaluate a possible correlation with disease severity and potential alterations in the plasma lipidomic profile of horses with asthma.<\/jats:p>","DOI":"10.2460\/ajvr.80.3.300","type":"journal-article","created":{"date-parts":[[2019,2,25]],"date-time":"2019-02-25T15:53:09Z","timestamp":1551109989000},"page":"300-305","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of lipid markers in surfactant obtained from asthmatic horses exposed to hay"],"prefix":"10.2460","volume":"80","author":[{"given":"Undine","family":"Christmann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. Duncan","family":"Hite","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharon G.","family":"Witonsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virginia A.","family":"Buechner-Maxwell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul L.","family":"Wood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"Pediatr Pulmonol","doi-asserted-by":"publisher","first-page":"794","DOI":"10.1002\/ppul.20657","article-title":"Developmental changes in rat surfactant lipidomics in the context of species variability","volume":"42","author":"Bernhard","year":"2007"},{"key":"ref2","series-title":"Integr Comp Biol","doi-asserted-by":"publisher","first-page":"610","DOI":"10.1093\/icb\/icm079","article-title":"The anatomy, physics, and physiology of gas exchange surfaces: is there a universal function for pulmonary surfactant in animal respiratory structures?","volume":"47","author":"Orgeig","year":"2007"},{"key":"ref3","series-title":"J Lipid Res","doi-asserted-by":"publisher","first-page":"1322","DOI":"10.1194\/jlr.M600054-JLR200","article-title":"Lipidomics of cellular and secreted phospholipids from differentiated human fetal type II alveolar epithelial cells","volume":"47","author":"Postle","year":"2006"},{"key":"ref4","series-title":"Sci Rep","doi-asserted-by":"publisher","first-page":"40555","DOI":"10.1038\/srep40555","article-title":"Lipidomics reveals dramatic lipid compositional changes in the maturing postnatal lung","volume":"7","author":"Dautel","year":"2017"},{"key":"ref5","series-title":"Chem Phys Lipids","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1016\/j.chemphyslip.2011.04.004","article-title":"Analysis of lung surfactant phosphatidylcholine metabolism in transgenic mice using stable isotopes","volume":"164","author":"Postle","year":"2011"},{"key":"ref6","series-title":"J Lipid Res","doi-asserted-by":"publisher","first-page":"926","DOI":"10.1194\/jlr.M074955","article-title":"Lipidomic characterization and localization of phospholipids in the human lung","volume":"58","author":"Zemski Berry","year":"2017"},{"key":"ref7","series-title":"Mol Biosyst","doi-asserted-by":"publisher","first-page":"3163","DOI":"10.1039\/C4MB00435C","article-title":"Alterations in rat pulmonary phosphatidylcholines after chronic exposure to ambient fine particulate matter","volume":"10","author":"Chen","year":"2014"},{"key":"ref8","series-title":"Am J Respir Crit Care Med","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1164\/rccm.201312-2210OC","article-title":"Untargeted lipidomic analysis in chronic obstructive pulmonary disease. Uncovering sphingolipids","volume":"190","author":"Telenga","year":"2014"},{"key":"ref9","series-title":"J Proteome Res","doi-asserted-by":"publisher","first-page":"3919","DOI":"10.1021\/pr5002059","article-title":"Novel approach for analysis of bronchoalveolar lavage fluid (BALF) using HPLCQTOF-MS-based lipidomics: lipid levels in asthmatics and corticosteroid-treated asthmatic patients","volume":"13","author":"Kang","year":"2014"},{"key":"ref10","series-title":"Clin Exp Allergy","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1111\/j.1365-2222.2006.02626.x","article-title":"Lysophosphatidic acid is detectable in human broncholaveolar lavage fluids at baseline and increased after segmental allergen challenge","volume":"37","author":"Georas","year":"2007"},{"key":"ref11","series-title":"Pulm Circ","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1086\/686311","article-title":"Plasma 12- and 15-hydroxyeicosanoids are predictors of survival in pulmonary arterial hypertension","volume":"6","author":"Al-Naamani","year":"2016"},{"key":"ref12","series-title":"PLoS One","doi-asserted-by":"publisher","first-page":"e0117985","DOI":"10.1371\/journal.pone.0117985","article-title":"Surfactant lipidomics in healthy children and childhood interstitial lung disease","volume":"10","author":"Griese","year":"2015"},{"key":"ref13","series-title":"J Immunol","doi-asserted-by":"publisher","first-page":"3870","DOI":"10.4049\/jimmunol.1601798","article-title":"Orchestrating role of apoptosis inhibitor of macrophage in the resolution of acute lung injury","volume":"199","author":"Kimura","year":"2017"},{"key":"ref14","series-title":"Cancer Res","doi-asserted-by":"publisher","first-page":"4608","DOI":"10.1158\/0008-5472.CAN-15-3403","article-title":"Myc expression drives aberrant lipid metabolism in lung cancer","volume":"76","author":"Hall","year":"2016"},{"key":"ref15","series-title":"Sci Rep","doi-asserted-by":"publisher","first-page":"11087","DOI":"10.1038\/s41598-017-11339-1","article-title":"Lipidomes of lung cancer and tumour-free lung tissues reveal distinct molecular signatures for cancer differentiation, age, inflammation, and pulmonary emphysema","volume":"7","author":"Eggers","year":"2017"},{"key":"ref16","series-title":"Biomark Med","doi-asserted-by":"publisher","first-page":"123","DOI":"10.2217\/bmm.15.93","article-title":"Polyunsaturated lysophosphatidic acid as a potential asthma biomarker","volume":"10","author":"Ackerman","year":"2016"},{"key":"ref17","series-title":"J Equine Vet Sci","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/j.jevs.2016.01.013","article-title":"Plasma lipidomic and inflammatory cytokine profiles of horses with equine metabolic syndrome","volume":"40","author":"Elzinga","year":"2016"},{"key":"ref18","series-title":"Theriogenology","doi-asserted-by":"publisher","first-page":"1212","DOI":"10.1016\/j.theriogenology.2016.04.012","article-title":"Lipidomics of equine sperm and seminal plasma: identification of amphiphilic (O-acyl)-omega-hydroxy-fatty acids","volume":"86","author":"Wood","year":"2016"},{"key":"ref19","series-title":"J Vet Med Res","first-page":"1071","article-title":"Lipidomics evaluation of the effects of feeding a yeast-based supplement containing algal docosahexaenoic acid (DHA) on DHA-containing glycerophospholipids in stallion spermatozoa","volume":"4","author":"Wood","year":"2017"},{"key":"ref20","series-title":"Theriogenology","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1016\/j.theriogenology.2017.09.012","article-title":"Lipidomics of equine amniotic fluid: identification of amphiphilic (O-acyl)-\u03c9-hydroxy-fatty acids","volume":"105","author":"Wood","year":"2018"},{"key":"ref21","series-title":"PLoS One","doi-asserted-by":"publisher","first-page":"e0193424","DOI":"10.1371\/journal.pone.0193424","article-title":"Lipid biomarkers of immune activation in equine leptospirosis and Leptospira-vaccinated horses","volume":"13","author":"Wood","year":"2018"},{"key":"ref22","series-title":"Arthritis Res Ther","doi-asserted-by":"publisher","first-page":"R123","DOI":"10.1186\/ar3427","article-title":"A targeted lipidomics approach to the study of eicosanoid release in synovial joints","volume":"13","author":"de Grauw","year":"2011"},{"key":"ref23","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"1452","DOI":"10.1111\/j.1939-1676.2008.0177.x","article-title":"Abnormalities in lung surfactant in horses clinically affected with recurrent airway obstruction (RAO)","volume":"22","author":"Christmann","year":"2008"},{"key":"ref24","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"468","DOI":"10.2460\/ajvr.71.4.468","article-title":"Surfactant alterations in horses with recurrent airway obstruction at various clinical stages","volume":"71","author":"Christmann","year":"2010"},{"key":"ref25","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1111\/j.1939-1676.2008.0269.x","article-title":"Role of lung surfactant in respiratory disease: current knowledge in large animal medicine","volume":"23","author":"Christmann","year":"2009"},{"key":"ref26","series-title":"Equine Vet J","first-page":"393","article-title":"Relationship between clinical signs and lung function in horses with recurrent airway obstruction (heaves) during a bronchodilator trial","volume":"32","author":"Robinson","year":"2000"},{"key":"ref27","series-title":"Can J Biochem Physiol","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1139\/y59-099","article-title":"A rapid method of total lipid extraction and purification","volume":"37","author":"Bligh","year":"1959"},{"key":"ref28","series-title":"Lipids","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1007\/BF02668129","article-title":"Quantitative analysis of phospholipids by thin-layer chromatography and phosphorus analysis of spots","volume":"1","author":"Rouser","year":"1966"},{"key":"ref29","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"612","DOI":"10.1111\/j.1939-1676.2009.0298.x","article-title":"Influence of age on surfactant isolated from healthy horses maintained on pasture","volume":"23","author":"Christmann","year":"2009"},{"key":"ref30","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"1402","DOI":"10.1111\/j.1939-1676.2006.tb00758.x","article-title":"Lung surfactant function and composition in neonatal foals and adult horses","volume":"20","author":"Christmann","year":"2006"},{"key":"ref31","series-title":"Biochim Biophys Acta","doi-asserted-by":"publisher","first-page":"612","DOI":"10.1016\/j.bbalip.2012.09.010","article-title":"Surfactant phospholipid metabolism","volume":"1831","author":"Agassandian","year":"2013"},{"key":"ref32","series-title":"Cell Mol Life Sci","doi-asserted-by":"publisher","first-page":"3931","DOI":"10.1007\/s00018-015-1982-3","article-title":"DAG tales: the multiple faces of diacylglycerol\u2014stereochemistry, metabolism, and signaling","volume":"72","author":"Eichmann","year":"2015"},{"key":"ref33","series-title":"Am J Vet Res","first-page":"495","article-title":"Alveolar clearance in horses with chronic obstructive pulmonary disease","volume":"60","author":"Votion","year":"1999"},{"key":"ref34","series-title":"Life Sci","doi-asserted-by":"publisher","first-page":"2185","DOI":"10.1016\/S0024-3205(99)00483-X","article-title":"Existence of a bioactive lipid, cyclic phosphatidic acid, bound to human serum albumin","volume":"65","author":"Kobayashi","year":"1999"},{"key":"ref35","series-title":"Biochem J","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1042\/bj2910677","article-title":"The bioactive phospholipid lysophosphatidic acid is released from activated platelets","volume":"291","author":"Eichholtz","year":"1993"},{"key":"ref36","series-title":"J Chromatogr B Analyt Technol Biomed Life Sci","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/j.jchromb.2018.01.002","article-title":"Quantitative determination of cyclic phosphatidic acid and its carba analog in mouse organs and plasma using LC-MS\/MS","volume":"1076","author":"Shimizu","year":"2018"},{"key":"ref37","series-title":"J Biol Chem","doi-asserted-by":"publisher","first-page":"31062","DOI":"10.1074\/jbc.M109.050617","article-title":"Diacylglycerol kinase epsilon is selective for both acyl chains of phosphatidic acid or diacylglycerol","volume":"284","author":"Lung","year":"2009"},{"key":"ref38","series-title":"Curr Pharm Biotechnol","doi-asserted-by":"publisher","first-page":"1026","DOI":"10.2174\/138920111795909087","article-title":"Lipid mediator profiling in pulmonary disease","volume":"12","author":"Lundstr\u00f6m","year":"2011"},{"key":"ref39","series-title":"Biochim Biophys Acta","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1016\/j.bbalip.2008.05.002","article-title":"Cyclic phosphatidic acid\u2014a unique bioactive phospholipid","volume":"1781","author":"Fujiwara","year":"2008"},{"key":"ref40","series-title":"Acta Biochim Pol","doi-asserted-by":"publisher","first-page":"227","DOI":"10.18388\/abp.2008_3070","article-title":"Lysophosphatidic acids, cyclic phosphatidic acids and autotaxin as promising targets in therapies of cancer and other diseases","volume":"55","author":"Gendaszewska-Darmach","year":"2008"},{"key":"ref41","series-title":"Eur J Pharmacol","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/j.ejphar.2006.05.017","article-title":"Cyclic phosphatidic acid stimulates respiration without producing vasopressor or tachycardiac effects in rats","volume":"543","author":"Hotta","year":"2006"},{"key":"ref42","series-title":"Am J Respir Crit Care Med","doi-asserted-by":"publisher","first-page":"928","DOI":"10.1164\/rccm.201306-1014OC","article-title":"Autotaxin production of lysophosphatidic acid mediates allergic asthmatic inflammation","volume":"188","author":"Park","year":"2013"},{"key":"ref43","series-title":"Am J Respir Cell Mol Biol","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1165\/rcmb.2015-0124OC","article-title":"Novel inhibitory effect of a lysophosphatidic acid 2 agonist on allergen-driven airway inflammation","volume":"54","author":"Knowlden","year":"2016"},{"key":"ref44","series-title":"JSM Alzheimer's Dis Relat Dement","first-page":"1001","article-title":"Increased lysophosphatidylethanolamine and diacylglycerol levels in Alzheimer's disease plasma","volume":"1","author":"Wood","year":"2014"}],"container-title":["American Journal of Veterinary Research"],"original-title":[],"link":[{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/ajvr\/80\/3\/ajvr.80.3.300.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/ajvr\/80\/3\/ajvr.80.3.300.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/ajvr\/80\/3\/ajvr.80.3.300.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T20:43:03Z","timestamp":1696538583000},"score":1,"resource":{"primary":{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/ajvr\/80\/3\/ajvr.80.3.300.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3]]},"references-count":44,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.2460\/ajvr.80.3.300","relation":{},"ISSN":["0002-9645"],"issn-type":[{"value":"0002-9645","type":"print"}],"subject":[],"published":{"date-parts":[[2019,3]]}}}