{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:01Z","timestamp":1792627201257,"version":"4.5.0"},"reference-count":42,"publisher":"American Veterinary Medical Association (AVMA)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:sec id=\"s001\">\n<jats:title>OBJECTIVE<\/jats:title>\n<jats:p>To quantify acute immunologic and metabolic responses of beef heifers following topical administration of transdermal flunixin meglumine (TDFM) at various times relative to bovine herpesvirus 1 (BHV1) and <jats:italic>Mannheimia haemolytica<\/jats:italic> challenges.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"s002\">\n<jats:title>ANIMALS<\/jats:title>\n<jats:p>32 beef heifers (mean body weight, 170 kg).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"s003\">\n<jats:title>PROCEDURES<\/jats:title>\n<jats:p>Heifers were assigned to 1 of 4 groups. Heifers in the control group did not receive TDFM, whereas 1 dose of TDFM (3.3 mg\/kg) was topically applied to heifers of groups A, V, and B at \u2212144, \u221272, and 0 hours. All heifers were inoculated with 1 \u00d7 10<jats:sup>8<\/jats:sup> plaque-forming units of BHV1 in each nostril at \u221272 hours and with 1.18 \u00d7 10<jats:sup>6<\/jats:sup> CFUs of <jats:italic>M haemolytica<\/jats:italic> intratracheally at 0 hours. Vaginal temperature was recorded and blood samples were collected for quantification of select immunologic and metabolic biomarkers at predetermined times from \u2212144 to 360 hours.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"s004\">\n<jats:title>RESULTS<\/jats:title>\n<jats:p>Mean vaginal temperature was similar between group A and the control group. Mean vaginal temperatures for groups V and B were generally lower than that for the control group following BHV1 and <jats:italic>M haemolytica<\/jats:italic> challenges, respectively. Mean neutrophil oxidative burst capacity and L-selectin expression at 0 hours were significantly decreased for group V relative to the other groups. Other biomarkers did not differ among the groups at any time.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"s005\">\n<jats:title>CONCLUSIONS AND CLINICAL RELEVANCE<\/jats:title>\n<jats:p>Results suggested that topical administration of TDFM to beef cattle effectively alleviated pyrexia without adverse effects on acute immunologic or metabolic responses when TDFM was administered at the same time as, but not before, respiratory pathogen challenge.<\/jats:p>\n<\/jats:sec>","DOI":"10.2460\/ajvr.81.3.243","type":"journal-article","created":{"date-parts":[[2020,2,26]],"date-time":"2020-02-26T16:06:12Z","timestamp":1582733172000},"page":"243-253","source":"Crossref","is-referenced-by-count":5,"title":["Acute immunologic and metabolic responses of beef heifers following topical administration of flunixin meglumine at various times relative to bovine herpesvirus 1 and Mannheimia haemolytica challenges"],"prefix":"10.2460","volume":"81","author":[{"given":"Alyssa B.","family":"Word","sequence":"first","affiliation":[{"name":"1Department of Animal and Food Sciences, College of Agricultural Sciences and Natural Resources, Texas Tech University, Lubbock, TX 79409."},{"name":"2Livestock Issues Research Unit, USDA Agricultural Research Service, Lubbock, TX 79403."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. Rand","family":"Broadway","sequence":"additional","affiliation":[{"name":"2Livestock Issues Research Unit, USDA Agricultural Research Service, Lubbock, TX 79403."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole C.","family":"Burdick Sanchez","sequence":"additional","affiliation":[{"name":"2Livestock Issues Research Unit, USDA Agricultural Research Service, Lubbock, TX 79403."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John P.","family":"Hutcheson","sequence":"additional","affiliation":[{"name":"3Merck Animal Health, Summit, NJ 07901."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy B.","family":"Ellis","sequence":"additional","affiliation":[{"name":"3Merck Animal Health, Summit, NJ 07901."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben P.","family":"Holland","sequence":"additional","affiliation":[{"name":"4Cactus Research Ltd, Amarillo, TX 79101."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Ballou","sequence":"additional","affiliation":[{"name":"1Department of Animal and Food Sciences, College of Agricultural Sciences and Natural Resources, Texas Tech University, Lubbock, TX 79409."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffery A.","family":"Carroll","sequence":"additional","affiliation":[{"name":"2Livestock Issues Research Unit, USDA Agricultural Research Service, Lubbock, TX 79403."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"Types and costs of respiratory disease treatments in US feedlots"},{"key":"ref2","series-title":"Acta Vet Scand","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1186\/BF03547031","article-title":"Local tissue damage in cows after intramuscular administration of preparations containing phenylbutazone, flunixin, ketoprofen, and 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