{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T12:17:38Z","timestamp":1762604258325},"reference-count":26,"publisher":"American Veterinary Medical Association (AVMA)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2021,6]]},"abstract":"Abstract<\/jats:title>\n\nOBJECTIVE<\/jats:title>\nTo evaluate whether mesenchymal stem cells (MSCs) can be safely administered IV to dogs with congestive heart failure (CHF) secondary to myxomatous mitral valve disease (MMVD) to improve cardiac function and prolong survival time.<\/jats:p>\n<\/jats:sec>\n\nANIMALS<\/jats:title>\n10 client-owned dogs with CHF secondary to MMVD.<\/jats:p>\n<\/jats:sec>\n\nPROCEDURES<\/jats:title>\nDogs with an initial episode of CHF secondary to MMVD were enrolled in a double-blind, placebo-controlled clinical trial. Five dogs in the MSC group received allogeneic Wharton jelly\u2013derived MSCs (2 X 106<\/jats:sup> cells\/kg, IV), and 5 dogs in the placebo group received a 1% solution of autologous serum (IV) for 3 injections 3 weeks apart. Cell-release criteria included trilineage differentiation, expression of CD44 and CD90 and not CD34 and major histocompatability complex class II, normal karyotype, and absence of contamination by pathogenic microorganisms. Patients were followed for 6 months or until death or euthanasia. Echocardiographic data, ECG findings, serum cardiac biomarker concentrations, CBC, and serum biochemical analysis results were obtained prior to and 4 hours after the first injection and every 3 months after the final injection.<\/jats:p>\n<\/jats:sec>\n\nRESULTS<\/jats:title>\nLymphocyte and eosinophil counts decreased significantly 4 hours after injection, and monocytes decreased significantly only in dogs that received an MSC injection. No significant differences were seen in the echocardiographic variables, ECG results, serum cardiac biomarker concentrations, survival time, and time to first diuretic drug dosage escalation between the 2 groups.<\/jats:p>\n<\/jats:sec>\n\nCONCLUSIONS AND CLINICAL RELEVANCE<\/jats:title>\nThis study showed that MSCs can be easily collected from canine Wharton jelly as an allogeneic source of MSCs and can be safely delivered IV to dogs with CHF secondary to MMVD.<\/jats:p>\n<\/jats:sec>","DOI":"10.2460\/ajvr.82.6.487","type":"journal-article","created":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T13:47:39Z","timestamp":1621950459000},"page":"487-493","source":"Crossref","is-referenced-by-count":11,"title":["Intravenous administration of allogeneic Wharton jelly\u2013derived mesenchymal stem cells for treatment of dogs with congestive heart failure secondary to myxomatous mitral valve disease"],"prefix":"10.2460","volume":"82","author":[{"given":"Vicky K.","family":"Yang","sequence":"first","affiliation":[{"name":"From the Department of Clinical Sciences, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA 01536 (Yang, Meola, Davis, Hoffman); and Department of Population and Quantitative Health Sciences, University of Massachusetts Medical School, Worcester, MA 01655 (Barton). Dr. Davis' present address is the Astellas Institute for Regenerative Medicine, Marlborough MA 01752. Dr. Hoffman's present address is the Office of the Dean, School of Veterinary Medicine, University of Pennsylvania..."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dawn M.","family":"Meola","sequence":"additional","affiliation":[{"name":"From the Department of Clinical Sciences, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA 01536 (Yang, Meola, Davis, Hoffman); and Department of Population and Quantitative Health Sciences, University of Massachusetts Medical School, Worcester, MA 01655 (Barton). Dr. Davis' present address is the Astellas Institute for Regenerative Medicine, Marlborough MA 01752. Dr. Hoffman's present address is the Office of the Dean, School of Veterinary Medicine, University of Pennsylvania..."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Airiel","family":"Davis","sequence":"additional","affiliation":[{"name":"From the Department of Clinical Sciences, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA 01536 (Yang, Meola, Davis, Hoffman); and Department of Population and Quantitative Health Sciences, University of Massachusetts Medical School, Worcester, MA 01655 (Barton). Dr. Davis' present address is the Astellas Institute for Regenerative Medicine, Marlborough MA 01752. Dr. Hoffman's present address is the Office of the Dean, School of Veterinary Medicine, University of Pennsylvania..."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce","family":"Barton","sequence":"additional","affiliation":[{"name":"From the Department of Clinical Sciences, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA 01536 (Yang, Meola, Davis, Hoffman); and Department of Population and Quantitative Health Sciences, University of Massachusetts Medical School, Worcester, MA 01655 (Barton). Dr. Davis' present address is the Astellas Institute for Regenerative Medicine, Marlborough MA 01752. Dr. Hoffman's present address is the Office of the Dean, School of Veterinary Medicine, University of Pennsylvania..."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew M.","family":"Hoffman","sequence":"additional","affiliation":[{"name":"From the Department of Clinical Sciences, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA 01536 (Yang, Meola, Davis, Hoffman); and Department of Population and Quantitative Health Sciences, University of Massachusetts Medical School, Worcester, MA 01655 (Barton). Dr. Davis' present address is the Astellas Institute for Regenerative Medicine, Marlborough MA 01752. Dr. Hoffman's present address is the Office of the Dean, School of Veterinary Medicine, University of Pennsylvania..."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"J Vet Cardiol","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/j.jvc.2012.01.006","article-title":"Myxomatous mitral valve disease in dogs: does size matter?","volume":"14","author":"Parker HG","year":"2012","unstructured":"Parker HG, Kilroy-Glynn P. Myxomatous mitral valve disease in dogs: does size matter? J Vet Cardiol 2012;14:19\u201329.10.1016\/j.jvc.2012.01.006"},{"key":"ref2","series-title":"J Vet Cardiol","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/j.jvc.2012.01.011","article-title":"Historical review, epidemiology and natural history of degenerative mitral valve disease","volume":"14","author":"Borgarelli M","year":"2012","unstructured":"Borgarelli M, Buchanan JW. 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