{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:09:15Z","timestamp":1791230955621,"version":"4.1.0"},"reference-count":47,"publisher":"American Veterinary Medical Association (AVMA)","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:sec id=\"s001\">\n<jats:title>OBJECTIVE<\/jats:title>\n<jats:p>To identify differential microRNA (miRNA) expression in dogs with splenic hemangiosarcoma, splenic hematoma, and histologically normal spleens.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"s002\">\n<jats:title>ANIMALS<\/jats:title>\n<jats:p>Dogs with splenic hemangiosarcoma (n = 10), splenic hematoma (n = 5), and histologically normal spleens (n = 5).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"s003\">\n<jats:title>PROCEDURES<\/jats:title>\n<jats:p>Splenic tissue and serum samples were collected from dogs with splenic masses (ie, hemangiosarcoma or hematoma samples) and healthy control dogs (ie, control samples), and total RNA was extracted. Reverse transcription quantitative real-time PCR was performed with 28 miRNAs associated with hemangiosarcoma, angiosarcoma, or associated genes. Differential expression analysis was performed.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"s004\">\n<jats:title>RESULTS<\/jats:title>\n<jats:p>Control tissue and serum samples had similar miRNA expression patterns, and hemangiosarcoma tissue and serum samples did not. Hemangiosarcoma serum samples had higher expression than hemangiosarcoma tissue for 13 miRNAs and lower expression for 1 miRNA. Control tissue and hemangiosarcoma tissue had varying expressions for 12 miRNAs, with 10 more highly expressed in control samples and 2 more highly expressed in hemangiosarcoma samples. Five miRNAs (miR-214-3p, miR-452, miR-494-3p, miR-497-5p, miR-543) had significantly different expression in serum between dogs with splenic masses (ie, hemangiosarcoma or hematoma) and serum of dogs with histologically normal spleens, with higher expression in the serum of dogs with splenic masses for all 5 miRNAs.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"s005\">\n<jats:title>CONCLUSIONS AND CLINICAL RELEVANCE<\/jats:title>\n<jats:p>5 circulating miRNAs were identified that distinguished dogs with splenic hemangiosarcoma or hematoma from those with histologically normal spleens. These 5 miRNAs had higher expression in dogs with splenic masses, indicating upregulation of these circulating miRNAs occurs in these splenic disease states. These miRNAs may be useful as a noninvasive screening tool that uses serum to identify dogs with splenic masses.<\/jats:p>\n<\/jats:sec>","DOI":"10.2460\/ajvr.82.8.659","type":"journal-article","created":{"date-parts":[[2021,7,23]],"date-time":"2021-07-23T13:52:19Z","timestamp":1627048339000},"page":"659-666","source":"Crossref","is-referenced-by-count":10,"title":["Identification of serum microRNAs with differential expression between dogs with splenic masses and healthy dogs with histologically normal spleens"],"prefix":"10.2460","volume":"82","author":[{"given":"Janet A.","family":"Grimes","sequence":"first","affiliation":[{"name":"Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA 30602."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelsey R.","family":"Robinson","sequence":"additional","affiliation":[{"name":"Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA 30602."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna-Claire M.","family":"Bullington","sequence":"additional","affiliation":[{"name":"Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA 30602."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer M.","family":"Schmiedt","sequence":"additional","affiliation":[{"name":"Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA 30602."},{"name":"Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA 30602."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1111\/j.1939-1676.2011.0695.x","article-title":"Mortality in North American dogs from 1984 to 2004: an investigation into age-, size-, and breed-related causes of death","volume":"25","author":"Fleming JM","year":"2011","unstructured":"Fleming JM, Creevy KE, Promislow DE. Mortality in North American dogs from 1984 to 2004: an investigation into age-, size-, and breed-related causes of death. J Vet Intern Med 2011;25:187\u2013198.\n10.1111\/j.1939-1676.2011.0695.x"},{"key":"ref2","series-title":"Withrow and MacEwen's small animal clinical oncology","first-page":"679","article-title":"Miscellaneous tumors: hemangiosarcoma","author":"Thamm DH","year":"2013","unstructured":"Thamm DH. Miscellaneous tumors: hemangiosarcoma. In: Withrow SJ, Vail DM, Page RL, eds. Withrow and MacEwen's small animal clinical oncology. 5th ed. St Louis: Elsevier\/Saunders, 2013;679\u2013687."},{"key":"ref3","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1111\/j.1939-1676.2004.tb00162.x","article-title":"Efficacy and toxicity of a dose-intensified doxorubicin protocol in canine hemangiosarcoma","volume":"18","author":"Sorenmo KU","year":"2004","unstructured":"Sorenmo KU, Baez JL, Clifford CA,  Efficacy and toxicity of a dose-intensified doxorubicin protocol in canine hemangiosarcoma. J Vet Intern Med 2004;18:209\u2013213.\n10.1111\/j.1939-1676.2004.tb00162.x"},{"key":"ref4","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1111\/j.1939-1676.1996.tb02085.x","article-title":"Surgery and doxorubicin in dogs with hemangiosarcoma","volume":"10","author":"Ogilvie GK","year":"1996","unstructured":"Ogilvie GK, Powers BE, Mallinckrodt CH,  Surgery and doxorubicin in dogs with hemangiosarcoma. J Vet Intern Med 1996;10:379\u2013384.\n10.1111\/j.1939-1676.1996.tb02085.x"},{"key":"ref5","series-title":"J Am Anim Hosp Assoc","doi-asserted-by":"publisher","first-page":"72","DOI":"10.5326\/0450072","article-title":"Prognosis for acute nontraumatic hemoperitoneum in the dog: a retrospective analysis of 60 cases (2003\u20132006)","volume":"45","author":"Aronsohn MG","year":"2009","unstructured":"Aronsohn MG, Dubiel B, Roberts B,  Prognosis for acute nontraumatic hemoperitoneum in the dog: a retrospective analysis of 60 cases (2003\u20132006). J Am Anim Hosp Assoc 2009;45:72\u201377.\n10.5326\/0450072"},{"key":"ref6","series-title":"J Am Vet Med Assoc","doi-asserted-by":"publisher","first-page":"553","DOI":"10.2460\/javma.232.4.553","article-title":"Prevalence of hemangiosarcoma in anemic dogs with a splenic mass and hemoperitoneum requiring a transfusion: 71 cases (2003\u20132005)","volume":"232","author":"Hammond TN","year":"2008","unstructured":"Hammond TN, Pesillo-Crosby SA. Prevalence of hemangiosarcoma in anemic dogs with a splenic mass and hemoperitoneum requiring a transfusion: 71 cases (2003\u20132005). J Am Vet Med Assoc 2008;232:553\u2013558.\n10.2460\/javma.232.4.553"},{"key":"ref7","series-title":"J Am Vet Med Assoc","first-page":"706","article-title":"Epidemiologic, clinical, pathologic, and prognostic characteristics of splenic hemangiosarcoma and splenic hematoma in dogs: 217 cases (1985)","volume":"193","author":"Prymak C","year":"1988","unstructured":"Prymak C, McKee LJ, Goldschmidt MH,  Epidemiologic, clinical, pathologic, and prognostic characteristics of splenic hemangiosarcoma and splenic hematoma in dogs: 217 cases (1985). J Am Vet Med Assoc 1988;193:706\u2013712."},{"key":"ref8","series-title":"J Am Vet Med Assoc","doi-asserted-by":"publisher","first-page":"1325","DOI":"10.2460\/javma.239.10.1325","article-title":"Mass-to-splenic volume ratio and splenic weight as a percentage of body weight in dogs with malignant and benign splenic masses: 65 cases (2007\u2013 2008)","volume":"239","author":"Mallinckrodt MJ","year":"2011","unstructured":"Mallinckrodt MJ, Gottfried SD. Mass-to-splenic volume ratio and splenic weight as a percentage of body weight in dogs with malignant and benign splenic masses: 65 cases (2007\u2013 2008). J Am Vet Med Assoc 2011;239:1325\u20131327.\n10.2460\/javma.239.10.1325"},{"key":"ref9","series-title":"Vet Radiol Ultrasound","doi-asserted-by":"publisher","first-page":"547","DOI":"10.1111\/j.1740-8261.2004.04094.x","article-title":"Contrast harmonic ultrasound of spontaneous liver nodules in 32 dogs","volume":"45","author":"O'Brien RT","year":"2004","unstructured":"O'Brien RT, Iani M, Matheson J,  Contrast harmonic ultrasound of spontaneous liver nodules in 32 dogs. Vet Radiol Ultrasound 2004;45:547\u2013553.\n10.1111\/j.1740-8261.2004.04094.x"},{"key":"ref10","series-title":"Vet Radiol Ultra-sound","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1111\/j.1740-8261.2004.04054.x","article-title":"Comparison between malignant and nonmalignant splenic masses in dogs using contrast-enhanced computed tomography","volume":"45","author":"Fife WD","year":"2004","unstructured":"Fife WD, Samii VF, Drost WT,  Comparison between malignant and nonmalignant splenic masses in dogs using contrast-enhanced computed tomography. Vet Radiol Ultra-sound 2004;45:289\u2013297.\n10.1111\/j.1740-8261.2004.04054.x"},{"key":"ref11","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"330","DOI":"10.1111\/j.1939-1676.2004.tb02554.x","article-title":"Magnetic resonance imaging of focal splenic and hepatic lesions in the dog","volume":"18","author":"Clifford CA","year":"2004","unstructured":"Clifford CA, Pretorius ES, Weisse C,  Magnetic resonance imaging of focal splenic and hepatic lesions in the dog. J Vet Intern Med 2004;18:330\u2013338.\n10.1111\/j.1939-1676.2004.tb02554.x"},{"key":"ref12","article-title":"Early detection of hemangiosarcoma","author":"Lamerato-Kozicki AR","year":"2013","unstructured":"Lamerato-Kozicki AR, Helm K, Modiano JF. Early detection of hemangiosarcoma, in Proceedings. 23rd Annu Forum Am Coll Vet Intern Med 2005. Available at: vin.com Accessed Jan 4 2013."},{"key":"ref13","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1111\/j.1939-1676.2001.tb01244.x","article-title":"Plasma vascular endothelial growth factor concentrations in healthy dogs and dogs with hemangiosarcoma","volume":"15","author":"Clifford CA","year":"2001","unstructured":"Clifford CA, Hughes D, Beal MW,  Plasma vascular endothelial growth factor concentrations in healthy dogs and dogs with hemangiosarcoma. J Vet Intern Med 2001;15:131\u2013 135.\n10.1111\/j.1939-1676.2001.tb01244.x"},{"key":"ref14","series-title":"Nat Rev Cancer","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1038\/nrc1840","article-title":"Oncomirs\u2014microRNAs with a role in cancer","volume":"6","author":"Esquela-Kerscher A","year":"2006","unstructured":"Esquela-Kerscher A, Slack FJ. Oncomirs\u2014microRNAs with a role in cancer. Nat Rev Cancer 2006;6:259\u2013269.\n10.1038\/nrc1840"},{"key":"ref15","series-title":"Cardiovasc Res","doi-asserted-by":"publisher","first-page":"553","DOI":"10.1093\/cvr\/cvn151","article-title":"MicroRNA: basic mechanisms and transcriptional regulatory networks for cell fate determination","volume":"79","author":"Fazi F","year":"2008","unstructured":"Fazi F, Nervi C. MicroRNA: basic mechanisms and transcriptional regulatory networks for cell fate determination. Cardiovasc Res 2008;79:553\u2013561.\n10.1093\/cvr\/cvn151"},{"key":"ref16","series-title":"Nature","doi-asserted-by":"publisher","first-page":"834","DOI":"10.1038\/nature03702","article-title":"MicroRNA expression profiles classify human cancers","volume":"435","author":"Lu J","year":"2005","unstructured":"Lu J, Getz G, Miska EA,  MicroRNA expression profiles classify human cancers. Nature 2005;435:834\u2013838.\n10.1038\/nature03702"},{"key":"ref17","series-title":"Nat Biotechnol","doi-asserted-by":"publisher","first-page":"462","DOI":"10.1038\/nbt1392","article-title":"MicroRNAs accurately identify cancer tissue origin","volume":"26","author":"Rosenfeld N","year":"2008","unstructured":"Rosenfeld N, Aharonov R, Meiri E,  MicroRNAs accurately identify cancer tissue origin. Nat Biotechnol 2008;26:462\u2013 469.\n10.1038\/nbt1392"},{"key":"ref18","series-title":"Br J Surg","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1002\/bjs.5673","article-title":"MicroRNAs and cancer","volume":"94","author":"Wijnhoven BP","year":"2007","unstructured":"Wijnhoven BP, Michael MZ, Watson DI. MicroRNAs and cancer. Br J Surg 2007;94:23\u201330.\n10.1002\/bjs.5673"},{"key":"ref19","series-title":"World J Gastrointest Pathophysiol","doi-asserted-by":"publisher","first-page":"74","DOI":"10.4291\/wjgp.v4.i4.74","article-title":"Pancreatic cancer diagnosis by free and exosomal miRNA","volume":"4","author":"Z\u00f6ller M","year":"2013","unstructured":"Z\u00f6ller M. Pancreatic cancer diagnosis by free and exosomal miRNA. World J Gastrointest Pathophysiol 2013;4:74\u201390.\n10.4291\/wjgp.v4.i4.74"},{"key":"ref20","series-title":"Clin Lab","first-page":"65","article-title":"Increased expression of miR-494 in serum of patients with prostate cancer and its potential diagnostic value","author":"Cai B","year":"2019","unstructured":"Cai B, Peng JH. Increased expression of miR-494 in serum of patients with prostate cancer and its potential diagnostic value. Clin Lab 2019;65."},{"key":"ref21","series-title":"Proc Natl Acad Sci U S A","doi-asserted-by":"publisher","first-page":"10513","DOI":"10.1073\/pnas.0804549105","article-title":"Circulating microRNAs as stable blood-based markers for cancer detection","volume":"105","author":"Mitchell PS","year":"2008","unstructured":"Mitchell PS, Parkin RK, Kroh EM,  Circulating microRNAs as stable blood-based markers for cancer detection. Proc Natl Acad Sci U S A 2008;105:10513\u201310518.\n10.1073\/pnas.0804549105"},{"key":"ref22","series-title":"Vet Pathol","doi-asserted-by":"publisher","first-page":"618","DOI":"10.1354\/vp.42-5-618","article-title":"Mutations of phosphatase and tensin homolog deleted from chromosome 10 in canine hemangiosarcoma","volume":"42","author":"Dickerson EB","year":"2005","unstructured":"Dickerson EB, Thomas R, Fosmire SP,  Mutations of phosphatase and tensin homolog deleted from chromosome 10 in canine hemangiosarcoma. Vet Pathol 2005;42:618\u2013632.\n10.1354\/vp.42-5-618"},{"key":"ref23","series-title":"J Vet Med Sci","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1292\/jvms.69.271","article-title":"The significance of p53 and retinoblastoma pathways in canine hemangiosarcoma","volume":"69","author":"Yonemaru K","year":"2007","unstructured":"Yonemaru K, Sakai H, Murakami M,  The significance of p53 and retinoblastoma pathways in canine hemangiosarcoma. J Vet Med Sci 2007;69:271\u2013278.\n10.1292\/jvms.69.271"},{"key":"ref24","series-title":"Vet Pathol","doi-asserted-by":"publisher","first-page":"971","DOI":"10.1354\/vp.43-6-971","article-title":"Expression of vascular endothelial growth factor, basic fibroblast growth factor, and their receptors (flt-1, flk-1, and flg-1) in canine vascular tumors","volume":"43","author":"Yonemaru K","year":"2006","unstructured":"Yonemaru K, Sakai H, Murakami M,  Expression of vascular endothelial growth factor, basic fibroblast growth factor, and their receptors (flt-1, flk-1, and flg-1) in canine vascular tumors. Vet Pathol 2006;43:971\u2013980.\n10.1354\/vp.43-6-971"},{"key":"ref25","series-title":"BMC Vet Res","doi-asserted-by":"publisher","first-page":"272","DOI":"10.1186\/s12917-016-0903-5","article-title":"A comparison of microRNA expression profiles from splenic hemangiosarcoma, splenic nodular hyperplasia, and normal spleens of dogs","volume":"12","author":"Grimes JA","year":"2016","unstructured":"Grimes JA, Prasad N, Levy S,  A comparison of microRNA expression profiles from splenic hemangiosarcoma, splenic nodular hyperplasia, and normal spleens of dogs. BMC Vet Res 2016;12:272.\n10.1186\/s12917-016-0903-5"},{"key":"ref26","article-title":"Guidelines for profiling biofluid miRNAs","year":"2020","unstructured":"Qiagen. Guidelines for profiling biofluid miRNAs. Available at: go.qiagen.com\/Guidelines-for-Profiling-Biofluid-miRNAs. Accessed Sep 18, 2020."},{"key":"ref27","series-title":"Int J Mol Sci","doi-asserted-by":"publisher","first-page":"25377","DOI":"10.3390\/ijms161025377","article-title":"MicroRNA-214 and microRNA-126 are potential biomarkers for malignant endothelial proliferative diseases","volume":"16","author":"Heishima K","year":"2015","unstructured":"Heishima K, Mori T, Ichikawa Y,  MicroRNA-214 and microRNA-126 are potential biomarkers for malignant endothelial proliferative diseases. Int J Mol Sci 2015;16:25377\u2013 25391.\n10.3390\/ijms161025377"},{"key":"ref28","series-title":"eLife","doi-asserted-by":"publisher","first-page":"e05005","DOI":"10.7554\/eLife.05005","article-title":"Predicting effective microRNA target sites in mammalian mRNAs","volume":"4","author":"Agarwal V","year":"2015","unstructured":"Agarwal V, Bell GW, Nam JW,  Predicting effective microRNA target sites in mammalian mRNAs. eLife 2015;4:e05005.\n10.7554\/eLife.05005"},{"key":"ref29","series-title":"Nat Methods","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1038\/nmeth.3485","article-title":"miRWalk2.0: a comprehensive atlas of microRNA-target interactions","volume":"12","author":"Dweep H","year":"2015","unstructured":"Dweep H, Gretz N. miRWalk2.0: a comprehensive atlas of microRNA-target interactions. Nat Methods 2015;12:697.\n10.1038\/nmeth.3485"},{"key":"ref30","series-title":"Nucleic Acids Res","doi-asserted-by":"publisher","first-page":"D155","DOI":"10.1093\/nar\/gky1141","article-title":"miRBase: from microRNA sequences to function","volume":"47","author":"Kozomara A","year":"2019","unstructured":"Kozomara A, Birgaoanu M, Griffiths-Jones S. miRBase: from microRNA sequences to function. Nucleic Acids Res 2019;47:D155\u2013D162.\n10.1093\/nar\/gky1141"},{"key":"ref31","series-title":"Genome Biol","doi-asserted-by":"publisher","first-page":"RESEARCH0034","DOI":"10.1186\/gb-2002-3-7-research0034","article-title":"Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes","volume":"3","author":"Vandesompele J","year":"2002","unstructured":"Vandesompele J, De Preter K, Pattyn F,  Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 2002;3:RESEARCH0034.\n10.1186\/gb-2002-3-7-research0034"},{"key":"ref32","series-title":"PLoS One","doi-asserted-by":"publisher","first-page":"e0137361","DOI":"10.1371\/journal.pone.0137361","article-title":"MicroRNA-214 promotes apoptosis in canine hemangiosarcoma by targeting the COP1-p53 axis","volume":"10","author":"Heishima K","year":"2015","unstructured":"Heishima K, Mori T, Sakai H,  MicroRNA-214 promotes apoptosis in canine hemangiosarcoma by targeting the COP1-p53 axis. PLoS One 2015;10:e0137361.\n10.1371\/journal.pone.0137361"},{"key":"ref33","series-title":"Cardiovasc Res","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1093\/cvr\/cvs003","article-title":"MicroRNA-214 inhibits angiogenesis by targeting Quaking and reducing angiogenic growth factor release","volume":"93","author":"van Mil A","year":"2012","unstructured":"van Mil A, Grundmann S, Goumans MJ,  MicroRNA-214 inhibits angiogenesis by targeting Quaking and reducing angiogenic growth factor release. Cardiovasc Res 2012;93:655\u2013665.\n10.1093\/cvr\/cvs003"},{"key":"ref34","series-title":"Mol Ther","doi-asserted-by":"publisher","first-page":"2008","DOI":"10.1016\/j.ymthe.2018.05.020","article-title":"Therapeutic silencing of miR-214 inhibits tumor progression in multiple mouse models","volume":"26","author":"Dettori D","year":"2018","unstructured":"Dettori D, Orso F, Penna E,  Therapeutic silencing of miR-214 inhibits tumor progression in multiple mouse models. Mol Ther 2018;26:2008\u20132018.\n10.1016\/j.ymthe.2018.05.020"},{"key":"ref35","series-title":"RNA Biol","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1080\/15476286.2015.1017205","article-title":"miR-214 promotes osteoclastogenesis by targeting Pten\/PI3k\/Akt pathway","volume":"12","author":"Zhao C","year":"2015","unstructured":"Zhao C, Sun W, Zhang P,  miR-214 promotes osteoclastogenesis by targeting Pten\/PI3k\/Akt pathway. RNA Biol 2015;12:343\u2013353.\n10.1080\/15476286.2015.1017205"},{"key":"ref36","series-title":"Technol Cancer Res Treat","doi-asserted-by":"publisher","first-page":"1136","DOI":"10.1177\/1533034617735931","article-title":"Mir-452\u20133p: a potential tumor promoter that targets the CPEB3\/EGFR axis in human hepatocellular carcinoma","volume":"16","author":"Tang H","year":"2017","unstructured":"Tang H, Zhang J, Yu Z,  Mir-452\u20133p: a potential tumor promoter that targets the CPEB3\/EGFR axis in human hepatocellular carcinoma. Technol Cancer Res Treat 2017;16:1136\u20131149.\n10.1177\/1533034617735931"},{"key":"ref37","series-title":"Res Vet Sci","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1016\/j.rvsc.2005.09.005","article-title":"Biological and molecular characterization of a canine hemangiosarcoma-derived cell line","volume":"81","author":"Thamm DH","year":"2006","unstructured":"Thamm DH, Dickerson EB, Akhtar N,  Biological and molecular characterization of a canine hemangiosarcoma-derived cell line. Res Vet Sci 2006;81:76\u201386.\n10.1016\/j.rvsc.2005.09.005"},{"key":"ref38","series-title":"Oncotarget","doi-asserted-by":"publisher","first-page":"58148","DOI":"10.18632\/oncotarget.11252","article-title":"miR-497\u20135p inhibits cell proliferation and invasion by targeting KCa3.1 in angiosarcoma","volume":"7","author":"Chen Y","year":"2016","unstructured":"Chen Y, Kuang D, Zhao X,  miR-497\u20135p inhibits cell proliferation and invasion by targeting KCa3.1 in angiosarcoma. Oncotarget 2016;7:58148\u201358161.\n10.18632\/oncotarget.11252"},{"key":"ref39","series-title":"Cell Cycle","doi-asserted-by":"publisher","first-page":"1621","DOI":"10.1080\/15384101.2020.1762037","article-title":"LncRNA NNT-AS1 promote glioma cell proliferation and metastases through miR-494\u20133p\/PRMT1 axis","volume":"19","author":"Zheng D","year":"2020","unstructured":"Zheng D, Chen D, Lin F,  LncRNA NNT-AS1 promote glioma cell proliferation and metastases through miR-494\u20133p\/PRMT1 axis. Cell Cycle 2020;19:1621\u20131631.\n10.1080\/15384101.2020.1762037"},{"key":"ref40","series-title":"Mol Med","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1186\/s10020-020-00175-1","article-title":"MiR-543 promotes tumorigenesis and angiogenesis in non-small cell lung cancer via modulating metastasis associated protein 1","volume":"26","author":"Wang D","year":"2020","unstructured":"Wang D, Cai L, Tian X,  MiR-543 promotes tumorigenesis and angiogenesis in non-small cell lung cancer via modulating metastasis associated protein 1. Mol Med 2020;26:44.\n10.1186\/s10020-020-00175-1"},{"key":"ref41","series-title":"J Cancer Res Clin Oncol","doi-asserted-by":"publisher","first-page":"809","DOI":"10.1007\/s00432-020-03172-3","article-title":"MicroRNAs that regulate PTEN as potential biomarkers in colorectal cancer: a systematic review","volume":"146","author":"Liu J","year":"2020","unstructured":"Liu J, Ke F, Chen T,  MicroRNAs that regulate PTEN as potential biomarkers in colorectal cancer: a systematic review. J Cancer Res Clin Oncol 2020;146:809\u2013820.\n10.1007\/s00432-020-03172-3"},{"key":"ref42","series-title":"Biosci Rep","doi-asserted-by":"publisher","first-page":"BSR20170798","DOI":"10.1042\/BSR20170798","article-title":"miR-494 up-regulates the PI3K\/Akt pathway via targeting PTEN and attenuates hepatic ischemia\/reperfusion injury in a rat model","volume":"37","author":"Su S","year":"2017","unstructured":"Su S, Luo D, Liu X,  miR-494 up-regulates the PI3K\/Akt pathway via targeting PTEN and attenuates hepatic ischemia\/reperfusion injury in a rat model. Biosci Rep 2017;37:BSR20170798.\n10.1042\/BSR20170798"},{"key":"ref43","series-title":"APMIS Suppl","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1111\/j.1600-0463.2008.125m3.x","article-title":"Canine vascular neoplasia\u2014histo-logic classification and inmunohistochemical analysis of 221 tumours and tumour-like lesions","volume":"125","author":"Gamlem H","year":"2008","unstructured":"Gamlem H, Nordstoga K. Canine vascular neoplasia\u2014histo-logic classification and inmunohistochemical analysis of 221 tumours and tumour-like lesions. APMIS Suppl 2008;125:19\u2013 40.\n10.1111\/j.1600-0463.2008.125m3.x"},{"key":"ref44","series-title":"Expert Rev Mol Diagn","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1586\/erm.11.11","article-title":"Diagnostic applications of cell-free and circulating tumor cell-associated miRNAs in cancer patients","volume":"11","author":"Mostert B","year":"2011","unstructured":"Mostert B, Sieuwerts AM, Martens JW,  Diagnostic applications of cell-free and circulating tumor cell-associated miRNAs in cancer patients. Expert Rev Mol Diagn 2011;11:259\u2013275.\n10.1586\/erm.11.11"},{"key":"ref45","series-title":"Int J Mol Sci","doi-asserted-by":"publisher","first-page":"4463","DOI":"10.3390\/ijms21124463","article-title":"Extracellular vesicles in cancer metastasis: potential as therapeutic targets and materials","volume":"21","author":"Kogure A","year":"2020","unstructured":"Kogure A, Yoshioka Y, Ochiya T. Extracellular vesicles in cancer metastasis: potential as therapeutic targets and materials. Int J Mol Sci 2020;21:4463.\n10.3390\/ijms21124463"},{"key":"ref46","series-title":"BioMed Res Int","doi-asserted-by":"publisher","first-page":"731479","DOI":"10.1155\/2015\/731479","article-title":"Challenges in using circulating miRNAs as cancer biomarkers","volume":"2015","author":"Tiberio P","year":"2015","unstructured":"Tiberio P, Callari M, Angeloni V,  Challenges in using circulating miRNAs as cancer biomarkers. BioMed Res Int 2015;2015:731479.\n10.1155\/2015\/731479"},{"key":"ref47","series-title":"Nucleic Acids Res","doi-asserted-by":"publisher","first-page":"7223","DOI":"10.1093\/nar\/gkr254","article-title":"Characterization of extracellular circulating microRNA","volume":"39","author":"Turchinovich A","year":"2011","unstructured":"Turchinovich A, Weiz L, Langheinz A,  Characterization of extracellular circulating microRNA. 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