{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:53:13Z","timestamp":1787025193830,"version":"build-2736575974"},"reference-count":22,"publisher":"American Veterinary Medical Association (AVMA)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["javma"],"published-print":{"date-parts":[[2002,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:bold>Objective<\/jats:bold>\n                    \u2014To develop a method for percutaneous\ncollection of fetal fluid from cattle in the late stages of\ngestation and determine whether bovine viral diarrhea\nvirus (BVDV) can be isolated from such fluids.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Design<\/jats:bold>\n                    \u2014Case series.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Animals<\/jats:bold>\n                    \u2014169 pregnant beef cattle.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Procedure<\/jats:bold>\n                    \u2014Animals were restrained in a squeeze\nchute, and hair was clipped from a region of the right\nflank. Pregnancy was confirmed, and fetal fluids were\nidentified by means of abdominal ultrasonography.\nFetal fluid was collected with a spinal needle. Virus\nisolation was performed on fetal fluids, WBC lysates\nfrom 160 live calves, and tissues from 12 calves that\ndied or were aborted. Blood samples collected from\nadult cattle were assayed with an immunoperoxidase\nmonolayer assay.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results<\/jats:bold>\n                    \u2014Fourteen animals aborted or delivered premature\ncalves within 3 weeks after fetal fluid collection;\nhowever, it could not be determined whether\nthis was a complication of the procedure or attributable\nto other factors. Results of BVDV isolation from\nfetal fluid samples were negative for 168 animals.\nHowever, a noncytopathic BVDV was isolated from\nfetal fluid obtained from a 2-year-old heifer; results of\nthe immunoperoxidase assay of serum from this\nheifer were also positive, and a noncytopathic BVDV\nwas isolated from tissue specimens from a stillborn\ncalf produced by this heifer.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\n                    \u2014Results suggest\nthat fetal fluids can be collected percutaneously\nfrom cattle in the late stages of gestation and that\nvirus isolation performed on fetal fluids can be used\nto identify fetuses infected with BVDV in utero.\nHowever, safety of the procedure could not be evaluated.\n(\n                    <jats:italic>J Am Vet Med Assoc<\/jats:italic>\n                    2002;220:1348\u20131352).\n                  <\/jats:p>","DOI":"10.2460\/javma.2002.220.1348","type":"journal-article","created":{"date-parts":[[2006,1,23]],"date-time":"2006-01-23T13:40:53Z","timestamp":1138023653000},"page":"1348-1352","source":"Crossref","is-referenced-by-count":10,"title":["Percutaneous collection of fetal fluids for detection of bovine viral diarrhea virus infection in cattle"],"prefix":"10.2460","volume":"220","author":[{"given":"Robert J.","family":"Callan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John A.","family":"Schnackel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hana Van","family":"Campen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert G.","family":"Mortimer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline A.","family":"Cavender","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth S.","family":"Williams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"key":"p_1_293","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1136\/vr.117.10.240","volume":"117","author":"Duffell SJ","year":"1985","journal-title":"Vet Rec"},{"key":"p_2_294","first-page":"449","volume":"172","author":"Coria MF","year":"1978","journal-title":"J Am Vet Med Assoc"},{"key":"p_3_295","first-page":"156","volume":"48","author":"McClurkin AW","year":"1984","journal-title":"Can J Comp Med"},{"key":"p_4_296","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1136\/vr.114.22.535","volume":"114","author":"Brownlie J","year":"1984","journal-title":"Vet Rec"},{"key":"p_5_297","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1007\/978-3-7091-9153-8_24","volume":"3","author":"Brock KV","year":"1991","journal-title":"Arch Virol Suppl"},{"key":"p_6_298","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1177\/104063879500700304","volume":"7","author":"Houe H","year":"1995","journal-title":"J Vet Diagn Invest"},{"key":"p_7_299","first-page":"23","volume":"38","author":"Taylor LF","year":"1997","journal-title":"Can Vet J"},{"key":"p_8_300","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5877(01)00181-7"},{"key":"p_9_301","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1177\/104063879801000213","volume":"10","author":"Van Campen H","year":"1998","journal-title":"J Vet Diagn Invest"},{"key":"p_10_302","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1128\/JCM.35.4.803-807.1997","volume":"35","author":"Saliki JT","year":"1997","journal-title":"J Clin Microbiol"},{"key":"p_11_303","doi-asserted-by":"crossref","first-page":"567","DOI":"10.7589\/0090-3558-33.3.567","volume":"33","author":"Van Campen H","year":"1997","journal-title":"J Wildl Dis"},{"key":"p_12_304","doi-asserted-by":"publisher","DOI":"10.1006\/mcpr.1998.0158"},{"key":"p_13_305","first-page":"502","volume":"127","author":"Vos PL","year":"1990","journal-title":"Vet Rec"},{"key":"p_14_306","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(97)00057-5"},{"key":"p_15_307","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/S0749-0720(15)30462-X","volume":"11","author":"Moennig V","year":"1995","journal-title":"Vet Clin North Am Food Anim Pract"},{"key":"p_16_308","first-page":"618","volume":"188","author":"Ross CE","year":"1986","journal-title":"J Am Vet Med Assoc"},{"key":"p_17_309","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1177\/104063870001200312","volume":"12","author":"Van Campen H","year":"2000","journal-title":"J Vet Diagn Invest"},{"key":"p_18_310","first-page":"29","volume":"38","author":"Taylor LF","year":"1997","journal-title":"Can Vet J"},{"key":"p_19_311","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/S0749-0720(15)30460-6","volume":"11","author":"Baker JC","year":"1995","journal-title":"Vet Clin North Am Food Anim Pract"},{"key":"p_20_312","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/S0749-0720(15)30465-5","volume":"11","author":"Houe H","year":"1995","journal-title":"Vet Clin North Am Food Anim Pract"},{"key":"p_21_313","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/S0749-0720(15)30461-8","volume":"11","author":"Bielefeldt-Ohmann H","year":"1995","journal-title":"Vet Clin North Am Food Anim Pract"},{"key":"p_22_314","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1136\/vr.114.23.558","volume":"114","author":"Duffell SJ","year":"1984","journal-title":"Vet Rec"}],"container-title":["Journal of the American Veterinary Medical Association"],"original-title":[],"link":[{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/javma\/220\/9\/javma.2002.220.1348.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/javma\/220\/9\/javma.2002.220.1348.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/javma\/220\/9\/javma.2002.220.1348.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T08:43:14Z","timestamp":1741336994000},"score":1,"resource":{"primary":{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/javma\/220\/9\/javma.2002.220.1348.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,5,1]]},"references-count":22,"journal-issue":{"issue":"9"},"URL":"https:\/\/doi.org\/10.2460\/javma.2002.220.1348","relation":{},"ISSN":["0003-1488"],"issn-type":[{"value":"0003-1488","type":"print"}],"subject":[],"published":{"date-parts":[[2002,5,1]]}}}