{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T10:43:27Z","timestamp":1775817807736,"version":"3.50.1"},"reference-count":26,"publisher":"American Veterinary Medical Association (AVMA)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["javma"],"published-print":{"date-parts":[[2003,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To determine whether cattle can become\npersistently infected with <jats:italic>Brucella suis<\/jats:italic> biovar 4,\nwhether the organism can be transmitted vertically or\nhorizontally, and whether tests for bovine brucellosis\nare diagnostic.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Design<\/jats:bold>\u2014Observational study.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Animals<\/jats:bold>\u201424 pregnant cows and their calves and 6\nbulls.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014Cows and bulls were housed separately\nin groups of 6 with each group consisting of 3 cattle\nexperimentally infected with <jats:italic>B suis<\/jats:italic> biovar 4 and 3\nna\u00efve animals. Cattle were observed for clinical signs\ndaily; blood samples were collected weekly. Clotted\nblood from each sample was submitted for bacterial\nculture. Serum was tested with an indirect ELISA and\nthe standard tube agglutination test (STAT), buffered\nplate agglutination test, brucellosis card test (BCT),\nand complement fixation test (CFT). Tissues collected\nat necropsy were submitted for bacterial culture and\nhistologic examination.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014All 15 inoculated cattle seroconverted on 2\nor more serologic tests, and bacteria were isolated\nfrom 4 inoculated cows at necropsy. There was no\nbacteriologic evidence of vertical or horizontal transmission,\nand none of the cattle developed clinical\nabnormalities or gross or histologic lesions. Results of\nthe indirect ELISA were positive for all inoculated cattle.\nThe other tests gave variable results; the CFT,\nSTAT, and BCT yielded negative results for at least 1\nof the 4 cattle from which the organism was isolated.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Results suggest\nthat cattle-to-cattle transmission of <jats:italic>B suis<\/jats:italic> biovar\n4 is unlikely. Serologic tests for bovine brucellosis\nshould be used cautiously when attempting to identify\ncattle with rangiferine brucellosis, as they do not\ndiscriminate between the 2 diseases and vary in their\nability to detect exposed cattle. 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