{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:16:28Z","timestamp":1787163388785,"version":"build-2736575974"},"reference-count":28,"publisher":"American Veterinary Medical Association (AVMA)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["javma"],"published-print":{"date-parts":[[2023,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>OBJECTIVE<\/jats:title>\n                    <jats:p>To evaluate the efficacy of the 3 major classes of anthelmintics used for the treatment of hookworms in dogs in the US and an extralabel treatment with an FDA-approved product for use in cats in a Labrador kennel with a history of persistent hookworm infections.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>ANIMALS<\/jats:title>\n                    <jats:p>22 dogs housed in a single kennel comprised of the following breeds: 19 Labrador Retrievers, 1 English Cocker Spaniel, 1 Chesapeake Bay Retriever, and 1 Boykin Spaniel.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>PROCEDURES<\/jats:title>\n                    <jats:p>We performed a fecal egg count (FEC) reduction test using 22 dogs that were allocated randomly to 1 of 5 treatment groups: pyrantel pamoate (Pyrantel pamoate suspension), fenbendazole (Safe-Guard suspension 10%), milbemycin oxime (Interceptor), moxidectin plus imidacloprid (Advantage Multi), and emodepside plus praziquantel (Profender topical solution for cats). FEC was performed on samples collected on days 0 and 11.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>FEC reductions for the milbemycin oxime, moxidectin plus imidacloprid, and emodepside plus praziquantel groups were 43.9%, 57.4%, and 100%, respectively. The FEC increased following treatment for the pyrantel and fenbendazole groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CLINICAL RELEVANCE<\/jats:title>\n                    <jats:p>\n                      These data demonstrate that the\n                      <jats:italic>Ancylostoma caninum<\/jats:italic>\n                      infecting the dogs in this kennel are highly resistant to all major anthelmintic classes approved for use in dogs in the US but are susceptible to emodepside. This was the first report of multiple anthelmintic drug\u2013resistant\n                      <jats:italic>A caninum<\/jats:italic>\n                      in a dog kennel that does not involve Greyhounds.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.2460\/javma.22.08.0377","type":"journal-article","created":{"date-parts":[[2022,12,15]],"date-time":"2022-12-15T11:47:41Z","timestamp":1671104861000},"page":"342-347","source":"Crossref","is-referenced-by-count":34,"title":["Multiple anthelmintic drug resistance in hookworms (Ancylostoma caninum) in a Labrador breeding and training kennel in Georgia, USA"],"prefix":"10.2460","volume":"261","author":[{"given":"Pablo D.","family":"Jimenez Castro","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA"},{"name":"Grupo de Parasitolog\u00eda Veterinaria, Universidad Nacional de Colombia, Bogot\u00e1, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kendra","family":"Durrence","sequence":"additional","affiliation":[{"name":"Taylor Farm Kennels, Sylvania, GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Durrence","sequence":"additional","affiliation":[{"name":"Taylor Farm Kennels, Sylvania, GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonor Sicalo","family":"Gianechini","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Collins","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA"},{"name":"Department of Molecular Biosciences, Weinberg College of Art and Sciences, Northwestern University, Evanston, IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kayla","family":"Dunn","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA"},{"name":"MySimplePetLab, Denver, CO"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ray M.","family":"Kaplan","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA"},{"name":"School of Veterinary Medicine, St. George\u2019s University, Grenada, West Indies"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"issue":"1-2","key":"ref1","series-title":"Vet Parasitol","first-page":"144","article-title":"Prevalence of intestinal parasites in pet dogs in the United States","volume":"166","author":"Little SE","year":"2009","unstructured":"Little SE, Johnson EM, Lewis D, et al. Prevalence of intestinal parasites in pet dogs in the United States. Vet Parasitol. 2009;166(1-2):144\u2013152.19716659"},{"key":"ref2","series-title":"NADA 141\u2013007. FDA","year":"1994","unstructured":"Drontal Plus\u2013Praziquantel, Pyrantel Pamoate, Febantel Tablet. NADA 141\u2013007. FDA; 1994."},{"key":"ref3","series-title":"NADA 141\u2013251. FDA","year":"2006","unstructured":"Advantage Multi for Dogs\u2013Imidacloprid, Moxidectin Solution. NADA 141\u2013251. FDA; 2006."},{"key":"ref4","series-title":"NADA 140\u2013915. FDA","year":"1996","unstructured":"Interceptor Flavor Tabs\u2013Milbemycin Oxime Tablet. NADA 140\u2013915. FDA; 1996."},{"key":"ref5","series-title":"NADA 121\u2013473. FDA","year":"1983","unstructured":"Panacur\u2013Fenbendazole Granule. NADA 121\u2013473. FDA; 1983."},{"key":"ref6","series-title":"NADA 140\u2013971. FDA","year":"1993","unstructured":"Heartgard Plus\u2013Ivermectin and Pyrantel Pamoate Tablet, Chewable. NADA 140\u2013971. FDA; 1993."},{"issue":"1","key":"ref7","series-title":"Parasit Vectors","first-page":"576","article-title":"Multiple drug resistance in the canine hookworm Ancylostoma caninum: an emerging threat?","volume":"12","author":"Jimenez Castro PD","year":"2019","unstructured":"Jimenez Castro PD, Howell SB, Schaefer JJ, Avramenko RW, Gilleard JS, Kaplan RM. Multiple drug resistance in the canine hookworm Ancylostoma caninum: an emerging threat? Parasit Vectors. 2019;12(1):576. doi:10.1186\/s13071\u2013019-3828\u2013631818311"},{"key":"ref8","series-title":"Int J Parasitol Drugs Drug Resist","first-page":"22","article-title":"Efficacy evaluation of anthelmintic products against an infection with the canine hookworm (Ancylostoma caninum) isolate Worthy 4.1F3P in dogs","volume":"13","author":"Jimenez Castro PD","year":"2020","unstructured":"Jimenez Castro PD, Mansour A, Charles S, et al. Efficacy evaluation of anthelmintic products against an infection with the canine hookworm (Ancylostoma caninum) isolate Worthy 4.1F3P in dogs. Int J Parasitol Drugs Drug Resist. 2020;13:22\u201327.32403053"},{"key":"ref9","series-title":"Int J Parasitol Drugs Drug Resist","first-page":"107","article-title":"Multiple drug resistance in hookworms infecting Greyhound dogs in the USA","volume":"17","author":"Jimenez Castro PD","year":"2021","unstructured":"Jimenez Castro PD, Venkatesan A, Redman E, et al. Multiple drug resistance in hookworms infecting Greyhound dogs in the USA. Int J Parasitol Drugs Drug Resist. 2021;17:107\u2013117.34492564"},{"issue":"6","key":"ref10","series-title":"J Vet Intern Med","first-page":"1243","article-title":"Results of a web-based health survey of retired racing Greyhounds","volume":"21","author":"Lord LK","year":"2007","unstructured":"Lord LK, Yaissle JE, Marin L, Couto CG. Results of a web-based health survey of retired racing Greyhounds. J Vet Intern Med. 2007;21(6):1243\u20131250.18196733"},{"issue":"18","key":"ref11","series-title":"S Afr Med J","first-page":"403","article-title":"Cutaneous larva migrans","volume":"40","author":"Leeming JA","year":"1966","unstructured":"Leeming JA. Cutaneous larva migrans. S Afr Med J. 1966;40(18):403\u2013405.5949465"},{"issue":"9","key":"ref12","series-title":"Acta Derm Venereol","first-page":"805","article-title":"Autochthonous cutaneous larva migrans in France and Europe","volume":"99","author":"Del Giudice P","year":"2019","unstructured":"Del Giudice P, Hakimi S, Vandenbos F, Magana C, Hubiche T. Autochthonous cutaneous larva migrans in France and Europe. Acta Derm Venereol. 2019;99(9):805\u2013808.31073620"},{"issue":"4","key":"ref13","series-title":"Trends Parasitol","first-page":"162","article-title":"Hookworms of dogs and cats as agents of cutaneous larva migrans","volume":"26","author":"Bowman DD","year":"2010","unstructured":"Bowman DD, Montgomery SP, Zajac AM, Eberhard ML, Kazacos KR. Hookworms of dogs and cats as agents of cutaneous larva migrans. Trends Parasitol. 2010;26(4):162\u2013167.20189454"},{"issue":"1","key":"ref14","series-title":"Emerg Infect Dis","first-page":"281","article-title":"In vivo observation of cutaneous larva migrans by fluorescence-advanced videodermatoscopy","volume":"27","author":"Ramondetta A","year":"2021","unstructured":"Ramondetta A, Ribero S, Quaglino P, Broganelli P. In vivo observation of cutaneous larva migrans by fluorescence-advanced videodermatoscopy. Emerg Infect Dis. 2021;27(1):281\u2013283.33350908"},{"issue":"1","key":"ref15","series-title":"Acta Trop","first-page":"23","article-title":"Human enteric infection with Ancylostoma caninum: hookworms reappraised in the light of a \u201cnew\u201d zoonosis","volume":"62","author":"Prociv P","year":"1996","unstructured":"Prociv P, Croese J. Human enteric infection with Ancylostoma caninum: hookworms reappraised in the light of a \u201cnew\u201d zoonosis. Acta Trop. 1996;62(1):23\u201344.8971276"},{"issue":"3","key":"ref16","series-title":"Am J Surg Pathol","first-page":"328","article-title":"Eosinophilic enteritis in northeastern Australia. Pathology, association with Ancylostoma caninum, and implications","volume":"19","author":"Walker NI","year":"1995","unstructured":"Walker NI, Croese J, Clouston AD, Parry M, Loukas A, Prociv P. Eosinophilic enteritis in northeastern Australia. Pathology, association with Ancylostoma caninum, and implications. Am J Surg Pathol. 1995;19(3):328\u2013337.7872431"},{"key":"ref17","series-title":"J Helminthol","first-page":"e39","article-title":"Molecular identification of hookworm isolates from stray dogs, humans, and selected wildlife from South Africa","volume":"94","author":"Ngcamphalala PI","unstructured":"Ngcamphalala PI, Lamb J, Mukaratirwa S. Molecular identification of hookworm isolates from stray dogs, humans, and selected wildlife from South Africa. J Helminthol. 2019;94:e39. doi:10.1017\/S0022149X1900013030789121"},{"issue":"1","key":"ref18","series-title":"Sci Rep","first-page":"3006","article-title":"Egg genotyping reveals the possibility of patent Ancylostoma caninum infection in human intestine","volume":"10","author":"Furtado LFV","year":"2020","unstructured":"Furtado LFV, de Oliveira Dias LT, de Oliveira Rodrigues T, Silva VJD, Oliveira VNGM, Rabelo \u00c9ML. Egg genotyping reveals the possibility of patent Ancylostoma caninum infection in human intestine. Sci Rep. 2020;10(1):3006. doi:10.1038\/s41598\u2013020-59874\u2013832080267"},{"issue":"3","key":"ref19","series-title":"J Am Anim Hosp Assoc","first-page":"160","article-title":"Combination anthelmintic treatment for persistent Ancylostoma caninum ova shedding in Greyhounds","volume":"55","author":"Hess LB","year":"2019","unstructured":"Hess LB, Millward LM, Rudinsky A, Vincent E, Marsh A. Combination anthelmintic treatment for persistent Ancylostoma caninum ova shedding in Greyhounds. J Am Anim Hosp Assoc. 2019;55(3):160\u2013166.30870603"},{"key":"ref20","series-title":"Clin Brief","article-title":"Persistent or suspected-resistant hookworm infections","author":"Jimenez Castro PD","unstructured":"Jimenez Castro PD, Kaplan RM. Persistent or suspected-resistant hookworm infections. Clin Brief. Published online August 2020. Accessed December 15, 2020. https:\/\/www.cliniciansbrief.com\/article\/persistent-or-suspected-resistant-hookworm-infections31818311"},{"issue":"1","key":"ref21","series-title":"Vet Clin North Am Food Anim Pract","first-page":"17","article-title":"Biology, epidemiology, diagnosis, and management of anthelmintic resistance in gastrointestinal nematodes of livestock","volume":"36","author":"Kaplan RM","year":"2020","unstructured":"Kaplan RM. Biology, epidemiology, diagnosis, and management of anthelmintic resistance in gastrointestinal nematodes of livestock. Vet Clin North Am Food Anim Pract. 2020;36(1):17\u201330.32029182"},{"key":"ref22","series-title":"Parasit Vectors","first-page":"356","article-title":"Mini-FLOTAC, a new tool for copromicroscopic diagnosis of common intestinal nematodes in dogs","volume":"7","author":"Maurelli MP","year":"2014","unstructured":"Maurelli MP, Rinaldi L, Alfano S, Pepe P, Coles GC, Cringoli G. Mini-FLOTAC, a new tool for copromicroscopic diagnosis of common intestinal nematodes in dogs. Parasit Vectors. 2014;7:356. doi:10.1186\/1756\u20133305-7\u201335625095701"},{"issue":"9","key":"ref23","series-title":"Parasitol Res","first-page":"3529","article-title":"A comparison of mini-FLOTAC and FLOTAC with classic methods to diagnosing intestinal parasites of dogs from Brazil","volume":"114","author":"Lima VFS","year":"2015","unstructured":"Lima VFS, Cringoli G, Rinaldi L, et al. A comparison of mini-FLOTAC and FLOTAC with classic methods to diagnosing intestinal parasites of dogs from Brazil. Parasitol Res. 2015;114(9):3529\u20133533.26122998"},{"key":"ref24","article-title":"R Package bayescount: power calculations and Bayesian analysis of count distributions and FECRT data using MCMC. Version 0.9.99\u20134","author":"Denwood M.","year":"2020","unstructured":"Denwood M. R Package bayescount: power calculations and Bayesian analysis of count distributions and FECRT data using MCMC. Version 0.9.99\u20134. Accessed December 15, 2020. https:\/\/cran.r-project.org\/web\/packages\/bayescount\/index.html"},{"issue":"3","key":"ref25","series-title":"Int J Parasitol Drugs Drug Resist","first-page":"386","article-title":"Modelling anthelmintic resistance by extending eggCounts package to allow individual efficacy","volume":"8","author":"Wang C","year":"2018","unstructured":"Wang C, Torgerson PR, Kaplan RM, George MM, Furrer R. Modelling anthelmintic resistance by extending eggCounts package to allow individual efficacy. Int J Parasitol Drugs Drug Resist. 2018;8(3):386\u2013393.30103206"},{"issue":"3\u20134","key":"ref26","series-title":"Vet Parasitol","first-page":"167","article-title":"The detection of anthelmintic resistance in nematodes of veterinary importance","volume":"136","author":"Coles GC","year":"2006","unstructured":"Coles GC, Jackson F, Pomroy WE, et al. The detection of anthelmintic resistance in nematodes of veterinary importance. Vet Parasitol. 2006;136(3\u20134):167\u2013185.16427201"},{"issue":"5","key":"ref27","series-title":"Int J Parasitol","first-page":"299","article-title":"Evaluating faecal egg count reduction using a specifically designed package \u201ceggCounts\u201d in R and a user friendly web interface","volume":"44","author":"Torgerson PR","year":"2014","unstructured":"Torgerson PR, Paul M, Furrer R. Evaluating faecal egg count reduction using a specifically designed package \u201ceggCounts\u201d in R and a user friendly web interface. Int J Parasitol. 2014;44(5):299\u2013303."},{"key":"ref28","series-title":"bioRxiv","first-page":"2022","article-title":"Molecular evidence of widespread benzimidazole drug resistance in Ancylostoma caninum from domestic dogs throughout the USA and discovery of a novel isotype-1 \u03b2-tubulin benzimidazole resistance mutation","author":"Venkatesan A","unstructured":"Venkatesan A, Jimenez Castro PD, Morosetti A, et al. Molecular evidence of widespread benzimidazole drug resistance in Ancylostoma caninum from domestic dogs throughout the USA and discovery of a novel isotype-1 \u03b2-tubulin benzimidazole resistance mutation. bioRxiv. 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