{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T18:48:38Z","timestamp":1791053318470,"version":"4.1.0"},"reference-count":52,"publisher":"American Veterinary Medical Association (AVMA)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["javma"],"published-print":{"date-parts":[[2006,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To identify the prevalence of DNA of <jats:italic>Mycoplasma haemofelis<\/jats:italic>; \u2018<jats:italic>Candidatus<\/jats:italic> Mycoplasma haemominutum\u2019; <jats:italic>Anaplasma phagocytophilum<\/jats:italic>; and species of <jats:italic>Bartonella, Neorickettsia,<\/jats:italic> and <jats:italic>Ehrlichia<\/jats:italic> in blood of cats used as blood donors in the United States. <jats:bold>Design<\/jats:bold>\u2014Prospective study.<\/jats:p>\n<jats:p><jats:bold>Animals<\/jats:bold>\u2014146 cats that were active blood donors.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014Environmental history was requested for each blood-donor cat from which a blood sample (mixed with EDTA) was available. Polymerase chain reaction assays capable of amplifying the DNA of the microorganisms of interest following DNA extraction from blood were performed.<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014Overall, DNA of one or more of the infectious agents was detected in blood samples from 16 of 146 (11%) feline blood donors. Twenty-eight laboratory-reared cats housed in a teaching hospital had negative results for DNA of all organisms investigated. The DNA of at least 1 infectious agent was amplified from blood samples collected from 16 of 118 (13.6%) community-source cats; assay results were positive for \u2018<jats:italic>Candidatus<\/jats:italic> M haemominutum,\u2019 <jats:italic>M haemofelis<\/jats:italic>, or <jats:italic>Bartonella henselae<\/jats:italic> alone or in various combinations. Of the community-source cats allowed outdoors (n = 61) or with known flea exposure (44), DNA for a hemoplasma or <jats:italic>B henselae<\/jats:italic> was detected in 21.3% and 22.7%, respectively.<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014When community-source cats, cats allowed outdoors, or cats exposed to fleas are to be used as blood donors, they should be regularly assessed for infection with <jats:italic>M haemofelis,<\/jats:italic> \u2018<jats:italic>Candidatus<\/jats:italic> M haemominutum,\u2019 and <jats:italic>Bartonella<\/jats:italic> spp, and flea-control treatment should be regularly provided.<\/jats:p>","DOI":"10.2460\/javma.229.5.700","type":"journal-article","created":{"date-parts":[[2006,9,1]],"date-time":"2006-09-01T13:54:45Z","timestamp":1157118885000},"page":"700-705","source":"Crossref","is-referenced-by-count":42,"title":["Prevalence of DNA of Mycoplasma haemofelis, \u2018Candidatus Mycoplasma haemominutum,\u2019 Anaplasma phagocytophilum, and species of Bartonella, Neorickettsia, and Ehrlichia in cats used as blood donors in the United States"],"prefix":"10.2460","volume":"229","author":[{"given":"Timothy B.","family":"Hackett","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wayne A.","family":"Jensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracy L.","family":"Lehman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne E.","family":"Hohenhaus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. Cynda","family":"Crawford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Urs","family":"Giger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Lappin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"N Engl J Med","first-page":"1764","article-title":"AIDS\u2014the first 20 years","volume":"344","author":"Sepkowitz","year":"2001"},{"key":"ref2","article-title":"AABB standards for infectious disease testing, 2006"},{"key":"ref3","series-title":"Textbook of veterinary internal medicine","first-page":"348","article-title":"Blood banking and transfusion medicine","author":"Hohenhaus","year":"2000"},{"key":"ref4","series-title":"J Feline Med Surg","first-page":"139","article-title":"Whole blood transfusions in 91 cats: a clinical evaluation","volume":"6","author":"Weingart","year":"2004"},{"key":"ref5","series-title":"J Vet Intern Med","first-page":"135","article-title":"ACVIM consensus statement. 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