{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T15:23:54Z","timestamp":1770218634130,"version":"3.49.0"},"reference-count":24,"publisher":"American Veterinary Medical Association (AVMA)","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["javma"],"published-print":{"date-parts":[[2006,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To develop a quantitative PCR assay for detection of <jats:italic>Borrelia burgdorferi<\/jats:italic> DNA in formalin-fixed, paraffin-embedded tissues; compare results of this assay with results of immunohistochemical staining of tissues from seropositive dogs; and determine whether <jats:italic>B burgdorferi<\/jats:italic> DNA could be detected in renal tissues from dogs with presumptive Lyme nephritis.<\/jats:p>\n<jats:p><jats:bold>Design<\/jats:bold>\u2014Cohort study.<\/jats:p>\n<jats:p><jats:bold>Sample Population<\/jats:bold>\u2014Archived tissue samples from 58 dogs.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014A quantitative PCR assay was performed on formalin-fixed, paraffin-embedded tissue sections from the dogs. Results were compared with results of immunohistochemical staining, <jats:italic>B burgdorferi<\/jats:italic> serostatus, clinical signs, and necropsy findings.<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u201438 dogs were classified as having positive or equivocal results for Lyme borreliosis, and 20 were classified as having negative results on the basis of clinical signs, serologic findings, and pathologic abnormalities. <jats:italic>Borrelia burgdorferi<\/jats:italic> DNA was amplified from tissue samples from only 4 (7%) dogs, all of which had been classified as having positive or equivocal results for Lyme borreliosis and had signs of presumptive Lyme nephritis. Results of PCR assays of renal tissue were positive for only 1 dog, and there was no agreement between results of immunohistochemical staining (ie, detection of <jats:italic>B burgdorferi<\/jats:italic> antigen) and results of the PCR assay (ie, detection of <jats:italic>B burgdorferi<\/jats:italic> DNA) for renal tissues.<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Results indicated that detection of <jats:italic>B burgdorferi<\/jats:italic> DNA in formalin-fixed, paraffin-embedded tissues is feasible, but that intact <jats:italic>B burgdorferi<\/jats:italic> DNA is rarely found in tissues from naturally infected dogs, even tissues from dogs with presumptive Lyme borreliosis. Further, findings support the contention that Lyme nephritis may be a sterile, immune complex disease.<\/jats:p>","DOI":"10.2460\/javma.229.8.1260","type":"journal-article","created":{"date-parts":[[2006,10,18]],"date-time":"2006-10-18T07:10:13Z","timestamp":1161155413000},"page":"1260-1265","source":"Crossref","is-referenced-by-count":23,"title":["Detection of Borrelia burgdorferi DNA in tissues from dogs with presumptive Lyme borreliosis"],"prefix":"10.2460","volume":"229","author":[{"given":"Jaime","family":"Chou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arno","family":"W\u00fcnschmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emir","family":"Hodzic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dori L.","family":"Borjesson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"Int J Syst Bacteriol","first-page":"378","article-title":"Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis","volume":"42","author":"Baranton","year":"1992"},{"key":"ref2","series-title":"J Clin Microbiol","first-page":"3670","article-title":"Detection of borreliacidal antibodies in dogs after challenge with Borrelia burgdorferi-infected Ixodes scapularis ticks","volume":"38","author":"Callister","year":"2000"},{"key":"ref3","series-title":"Cornell Vet","first-page":"253","article-title":"Lyme borreliosis in cattle and horses: a review of the literature","volume":"82","author":"Parker","year":"1992"},{"key":"ref4","series-title":"J Infect Dis","first-page":"651","article-title":"Experimental Lyme disease in dogs produces arthritis and 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