{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T20:40:13Z","timestamp":1792010413113,"version":"4.4.0"},"reference-count":41,"publisher":"American Veterinary Medical Association (AVMA)","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["javma"],"published-print":{"date-parts":[[2011,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Case Description<\/jats:bold>\u2014A 10-month-old Boxer was evaluated for fever and signs of cervical pain.<\/jats:p>\n<jats:p><jats:bold>Clinical Findings<\/jats:bold>\u2014Physical examination revealed lethargy, fever, and mucopurulent ocular and preputial discharge. On neurologic examination, the gait was characterized by a short stride. The dog kept its head flexed and resisted movement of the neck, consistent with cervical pain. Clinicopathologic findings included neutrophilic leukocytosis, a left shift, and monocytosis. Cervical radiographs were unremarkable. Cerebrospinal fluid analysis revealed neutrophilic pleocytosis and high total protein content. On the basis of signalment, history, and clinicopathologic data, a diagnosis of steroid-responsive meningitis-arteritis was made.<\/jats:p>\n<jats:p><jats:bold>Treatment and Outcome<\/jats:bold>\u2014The dog was treated with prednisone (3.2 mg\/kg [1.45 mg\/lb], PO, q 24 h), for 3 weeks with limited response. Consequently, azathioprine (2 mg\/kg [0.9 mg\/lb], PO, q 24 h) was administered. Three weeks later, the dog was evaluated for tachypnea and lethargy. Complete blood count revealed leukopenia, neutropenia, and a left shift. Thoracic radiography revealed a diffuse bronchointerstitial pattern. The dog subsequently went into respiratory arrest and died. On histologic evaluation, amoebic organisms were observed in the lungs, kidneys, and meninges of the brain and spinal cord. A unique <jats:italic>Acanthamoeba<\/jats:italic> sp was identified by use of PCR assay.<\/jats:p>\n<jats:p><jats:bold>Clinical Relevance<\/jats:bold>\u2014This dog developed systemic amoebic infection presumed to be secondary to immunosuppression. The development of secondary infection should be considered in animals undergoing immunosuppression for immune-mediated disease that develop clinical signs unrelated to the primary disease. Although uncommon, amoebic infection may develop in immunosuppressed animals. Use of a PCR assay for identification of <jats:italic>Acanthamoeba<\/jats:italic> spp may provide an antemortem diagnosis.<\/jats:p>","DOI":"10.2460\/javma.238.11.1476","type":"journal-article","created":{"date-parts":[[2011,6,1]],"date-time":"2011-06-01T07:51:37Z","timestamp":1306914697000},"page":"1476-1481","source":"Crossref","is-referenced-by-count":30,"title":["Multisystemic infection with an Acanthamoeba sp in a dog"],"prefix":"10.2460","volume":"238","author":[{"given":"Marc","family":"Kent","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon R.","family":"Platt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel R.","family":"Rech","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph S.","family":"Eagleson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth W.","family":"Howerth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan","family":"Shoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul A.","family":"Fuerst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greg","family":"Booton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Govinda S.","family":"Visvesvara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott J.","family":"Schatzberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"J Comp Pathol","first-page":"39","article-title":"Hydranencephaly and cerebellar hypoplasia in two kittens attributed to intrauterine parvovirus infection","volume":"121","author":"Sharp","year":"1999"},{"key":"ref2","series-title":"Vet Parasitol","first-page":"315","article-title":"Detection of Leishmania infantum DNA by fret-based real-time PCR in urine from dogs with natural clinical leishmaniosis","volume":"147","author":"Solano-Gallego","year":"2007"},{"key":"ref3","series-title":"Naegleria fowleri. J Clin Microbiol","first-page":"3589","article-title":"Multiplex realtime PCR assay for simultaneous detection of Acanthamoeba spp., Balamuthia mandrillaris, and","volume":"44","author":"Qvarnstrom","year":"2006"},{"key":"ref4","series-title":"Vet Parasitol","first-page":"74","article-title":"In vitro culture, serologic and molecular analysis of Acanthamoeba isolated from the liver of a keel-billed toucan (Ramphastos sulfuratus)","volume":"143","author":"Visvesvara","year":"2007"},{"key":"ref5","series-title":"Int J Parasitol","first-page":"1001","article-title":"Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals","volume":"34","author":"Schuster","year":"2004"},{"key":"ref6","series-title":"A new key to freshwater and soil gymnamoebae with instructions for culture","author":"Page","year":"1988"},{"key":"ref7","series-title":"Science","first-page":"1541","article-title":"Naegleria fowleri: trimethoprim sensitivity","volume":"209","author":"Cerva","year":"1980"},{"key":"ref8","series-title":"J Clin Microbiol","first-page":"1621","article-title":"18S ribosomal DNA typing and tracking of Acanthamoeba species isolates from corneal scrape specimens, contact lenses, lens cases, and home water supplies of Acanthamoeba keratitis patients in Hong Kong","volume":"40","author":"Booton","year":"2002"},{"key":"ref9","series-title":"Brief Bioinform","first-page":"150","article-title":"MEGA3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment","volume":"5","author":"Kumar","year":"2004"},{"key":"ref10","series-title":"J Am Anim Hosp Assoc","first-page":"677","article-title":"Corticosteroid-responsive meningitis in ten dogs","volume":"21","author":"Meric","year":"1985"},{"key":"ref11","series-title":"J Small Anim Pract","first-page":"311","article-title":"Steroid responsive meningitis-arteritis in dogs: long-term study of 32 cases","volume":"35","author":"Tipold","year":"1994"},{"key":"ref12","series-title":"J Vet Intern Med","first-page":"269","article-title":"Steroid-responsive meningitis-arteritis in dogs with noninfectious, nonerosive, idiopathic, immune-mediated polyarthritis","volume":"16","author":"Webb","year":"2002"},{"key":"ref13","series-title":"J Am Anim Hosp Assoc","first-page":"324","article-title":"Long-term outcome of five cases of corticosteroid-responsive meningomyelitis","volume":"26","author":"Irving","year":"1990"},{"key":"ref14","series-title":"J Am Anim Hosp Assoc","first-page":"277","article-title":"Aseptic suppurative meningitis in juvenile Boxer dogs: retrospective study of 12 cases","volume":"42","author":"Behr","year":"2006"},{"key":"ref15","series-title":"Euro J Comp Anim Pract","first-page":"24","article-title":"Aseptic suppurative meningitis in Bernese Mountain Dogs","volume":"2","author":"Presthus","year":"1991"},{"key":"ref16","series-title":"Vet Pathol","first-page":"337","article-title":"Pathologic features of naturally occurring juvenile polyarteritis in Beagle dogs","volume":"32","author":"Snyder","year":"1995"},{"key":"ref17","series-title":"J Am Anim Hosp Assoc","first-page":"459","article-title":"Necrotizing vasculitis of the spinal pachyleptomeningeal arteries in three Bernese Mountain Dog littermates","volume":"22","author":"Meric","year":"1986"},{"key":"ref18","series-title":"J Small Anim Pract","first-page":"295","article-title":"Long-term treatment of dogs with steroid-responsive meningitis-arteritis: clinical, laboratory and therapeutic results","volume":"41","author":"Cizinauskas","year":"2000"},{"key":"ref19","series-title":"J Am Vet Med Assoc","first-page":"239","article-title":"Frequency of urinary tract infection among dogs with pruritic disorders receiving long-term glucocorticoid treatment","volume":"227","author":"Torres","year":"2005"},{"key":"ref20","series-title":"J Am Vet Med Assoc","first-page":"43","article-title":"Urinary tract infection associated with long-term corticosteroid administration in dogs with chronic skin diseases","volume":"186","author":"Ihrke","year":"1985"},{"key":"ref21","series-title":"Vet J","first-page":"675","article-title":"Brain abscess in a dog immunosuppressed using cyclosporin","volume":"173","author":"Smith","year":"2007"},{"key":"ref22","series-title":"J Am Vet Med Assoc","first-page":"948","article-title":"Evaluation of the prevalence of infections in cats after renal transplantation: 169 cases (1987\u20132003)","volume":"227","author":"Kadar","year":"2005"},{"key":"ref23","series-title":"Parasitol Res","first-page":"423","article-title":"Multifocal Balamuthia mandrillaris infection in a dog in Australia","volume":"100","author":"Finnin","year":"2007"},{"key":"ref24","series-title":"Vet Pathol","first-page":"506","article-title":"Disseminated infection with Balamuthia mandrillaris in a dog","volume":"41","author":"Foreman","year":"2004"},{"key":"ref25","series-title":"Brain Pathol","first-page":"583","article-title":"Free-living, amphizoic and opportunistic amebas","volume":"7","author":"Martinez","year":"1997"},{"key":"ref26","series-title":"Sappinia diploidea. FEMS Immunol Med Microbiol","first-page":"1","article-title":"Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and","volume":"50","author":"Visvesvara","year":"2007"},{"key":"ref27","series-title":"Surv Ophthalmol","first-page":"493","article-title":"Acanthamoeba keratitis","volume":"42","author":"Illingworth","year":"1998"},{"key":"ref28","series-title":"Naegleria fowleri, Annu Rev Microbiol","first-page":"101","article-title":"Primary amebic meningoencephalitis and the biology of","volume":"36","author":"John","year":"1982"},{"key":"ref29","series-title":"J Vet Intern Med","first-page":"230","article-title":"Granulomatous amebic meningoencephalitis causing the syndrome of inappropriate secretion of antidiuretic hormone in a dog","volume":"17","author":"Brofman","year":"2003"},{"key":"ref30","series-title":"Vet Parasitol","first-page":"183","article-title":"Disseminated Acanthamoeba sp. infection in a dog","volume":"128","author":"Dubey","year":"2005"},{"key":"ref31","series-title":"Naegleria fowleri. J Vet Diagn Invest","first-page":"605","article-title":"Seasonal meningoencephalitis in Holstein cattle caused by","volume":"17","author":"Daft","year":"2005"},{"key":"ref32","series-title":"Vet Pathol","first-page":"239","article-title":"Primary amebic meningoencephalitis due to Naegleria fowleri in a South American tapir","volume":"34","author":"Lozano-Alarc\u00f3n","year":"1997"},{"key":"ref33","series-title":"Microbial Pathogenesis","first-page":"277","article-title":"Pathogenesis of Acanthamoeba infections","volume":"34","author":"Ahmed Khan","year":"2003"},{"key":"ref34","series-title":"J Am Vet Med Assoc","first-page":"951","article-title":"Amebic meningoencephalitis caused by Acanthamoeba castellani in a dog","volume":"187","author":"Pearce","year":"1985"},{"key":"ref35","series-title":"J Infect Dis","first-page":"692","article-title":"Experimental pneumonitis and encephalitis caused by acanthamoeba in mice: pathogenesis and ultrastructural features","volume":"131","author":"Martinez","year":"1975"},{"key":"ref36","series-title":"Drug Resist Updat","first-page":"41","article-title":"Opportunistic amoebae: challenges in prophylaxis and treatment","volume":"7","author":"Schuster","year":"2004"},{"key":"ref37","series-title":"Vet Parasitol","first-page":"91","article-title":"Amebae and ciliated protozoa as causal agents of waterborne zoonotic disease","volume":"126","author":"Schuster","year":"2004"},{"key":"ref38","series-title":"J Clin Microbiol","first-page":"1903","article-title":"Use of subgenic 18S ribosomal DNA PCR and sequencing for genus and genotype identification of acanthamoebae from humans with keratitis and from sewage sludge","volume":"39","author":"Schroeder","year":"2001"},{"key":"ref39","series-title":"J Protozool","first-page":"378","article-title":"Identification of Acanthamoeba at the generic and specific levels using the polymerase chain reaction","volume":"39","author":"Vodkin","year":"1992"},{"key":"ref40","series-title":"Emerg Infect Dis","first-page":"1144","article-title":"Acanthamoeba spp. in urine of critically ill patients","volume":"15","author":"Santos","year":"2009"},{"key":"ref41","series-title":"J Clin Microbiol","first-page":"3232","article-title":"Validation of realtime PCR for laboratory diagnosis of Acanthamoeba keratitis","volume":"46","author":"Thompson","year":"2008"}],"container-title":["Journal of the American Veterinary Medical Association"],"original-title":[],"link":[{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/javma\/238\/11\/javma.238.11.1476.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/javma\/238\/11\/javma.238.11.1476.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/javma\/238\/11\/javma.238.11.1476.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T13:17:19Z","timestamp":1741353439000},"score":1,"resource":{"primary":{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/javma\/238\/11\/javma.238.11.1476.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,6,1]]},"references-count":41,"journal-issue":{"issue":"11"},"URL":"https:\/\/doi.org\/10.2460\/javma.238.11.1476","relation":{},"ISSN":["0003-1488"],"issn-type":[{"value":"0003-1488","type":"print"}],"subject":[],"published":{"date-parts":[[2011,6,1]]}}}