{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:38:28Z","timestamp":1792003108336,"version":"4.4.0"},"reference-count":22,"publisher":"American Veterinary Medical Association (AVMA)","issue":"9","license":[{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["javma"],"published-print":{"date-parts":[[2024,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:sec>\n<jats:title>OBJECTIVES<\/jats:title>\n<jats:p>To measure the effect of routine vaccination on serum amyloid A (SAA) concentration in apparently healthy horses. We hypothesized that routine vaccination would increase SAA in healthy horses.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>ANIMALS<\/jats:title>\n<jats:p>21 apparently healthy client-owned horses and 15 Kansas State University College of Veterinary Medicine\u2013owned horses.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>METHODS<\/jats:title>\n<jats:p>In experiment 1 (n = 8 horses), a blinded, randomized, prospective, crossover study was performed. Horses were either vaccinated (rabies, tetanus, West Nile, Eastern and Western equine encephalomyelitis, equine herpesvirus-1\/-4, influenza) or administered saline, and SAA was measured at 6, 12, and 24 hours and daily until day 10 with a commercial lateral-flow immunoassay. In experiment 2 (n = 28 horses), a prospective, observational study measured SAA after vaccination at 12 and 24 hours and daily until day 10. A linear mixed-effect model with repeated measures over time blocked by horse tested the effect of treatment on SAA. A repeated-measures correlation tested the correlation between SAA and temperature.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>RESULTS<\/jats:title>\n<jats:p>Over time, vaccinated horses had increased model-adjusted SAA compared to unvaccinated horses without clinical evidence of adverse reaction (<jats:italic>P<\/jats:italic> &lt; .01). In experiment 1, the model-adjusted SAA after vaccination peaked on day 2 (median, 1,872 \u00b5g\/mL; IQR, 1,220.8 to 2,402.5 \u00b5g\/mL) and returned to normal (&lt; 20 \u00b5g\/mL) by day 9 (median, 6 \u00b5g\/mL; IQR, 0.8 to 23.5 \u00b5g\/mL) after vaccination. In experiment 2, vaccinated horses had increased SAA over time; temperature and SAA were not correlated (<jats:italic>P<\/jats:italic> = .78).<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>CLINICAL RELEVANCE<\/jats:title>\n<jats:p>Results of this study indicated that routine vaccination results in increased SAA concentration and provided evidence for a period of convalescence following vaccination. Measuring SAA for 10 days following vaccination cannot be used as an indicator of illness.<\/jats:p>\n<\/jats:sec>","DOI":"10.2460\/javma.24.04.0244","type":"journal-article","created":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T12:59:04Z","timestamp":1720011544000},"page":"1181-1187","source":"Crossref","is-referenced-by-count":4,"title":["Serum amyloid A increases following routine vaccination of healthy adult horses"],"prefix":"10.2460","volume":"262","author":[{"given":"Cassandra M.","family":"Baumgarten","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0259-7210","authenticated-orcid":true,"given":"Katherine M.","family":"Delph Miller","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth G.","family":"Davis","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurie A.","family":"Beard","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher A.","family":"Blevins","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margo","family":"Wottowa","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Hill","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert L.","family":"Larson","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"issue":"2","key":"ref1","series-title":"Vet Clin North Am Equine Pract","first-page":"285","article-title":"Blood proteins and inflammation in the horse","volume":"24","author":"Crisman MV","year":"2008","unstructured":"Crisman MV, Scarratt WK, Zimmerman KL. 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J Vet Diagn Invest. 2022;34(1):42-53. doi:10.1177\/10406387211056029"}],"container-title":["Journal of the American Veterinary Medical Association"],"original-title":[],"link":[{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/javma\/262\/9\/javma.24.04.0244.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/javma\/262\/9\/javma.24.04.0244.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/javma\/262\/9\/javma.24.04.0244.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T13:07:50Z","timestamp":1741352870000},"score":1,"resource":{"primary":{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/javma\/262\/9\/javma.24.04.0244.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,1]]},"references-count":22,"journal-issue":{"issue":"9"},"URL":"https:\/\/doi.org\/10.2460\/javma.24.04.0244","relation":{},"ISSN":["0003-1488"],"issn-type":[{"value":"0003-1488","type":"print"}],"subject":[],"published":{"date-parts":[[2024,9,1]]}}}