{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T01:12:09Z","timestamp":1790298729113,"version":"4.1.0"},"reference-count":35,"publisher":"American Veterinary Medical Association (AVMA)","issue":"11","license":[{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["javma"],"published-print":{"date-parts":[[2024,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:sec>\n<jats:title>OBJECTIVE<\/jats:title>\n<jats:p>Evaluate the incidence of <jats:italic>Borrelia burgdorferi<\/jats:italic> in cases of equine nuchal bursitis (NB) and investigate the relationship between elevated serum outer surface protein A (OspA) antibodies and the molecular identification of <jats:italic>B burgdorferi<\/jats:italic> in bursal tissue or synovial fluid. Additionally, describe clinical cases and compare the histologic changes in NB with and without detection of <jats:italic>B burgdorferi<\/jats:italic>.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>METHODS<\/jats:title>\n<jats:p>This was a retrospective multicenter cohort study (2013 to 2022). Medical records from horses with a diagnosis of NB and <jats:italic>B burgdorferi<\/jats:italic> PCR testing on NB tissue or synovial fluid were reviewed. The study population included 11 horses with a postmortem diagnosis of NB, 19 horses from the northeastern US with an antemortem diagnosis of <jats:italic>B burgdorferi<\/jats:italic> PCR\u2013positive NB, and 15 healthy controls without evidence of NB and unvaccinated for <jats:italic>B burgdorferi<\/jats:italic>. Where serum was available, Lyme multiplex assay results were compared with controls and ELISAs targeting individual <jats:italic>B burgdorferi<\/jats:italic> antigens were performed. Histologic findings in nuchal bursa tissue were compared between NB cases with and without <jats:italic>B burgdorferi<\/jats:italic> PCR detection.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>RESULTS<\/jats:title>\n<jats:p>Serum OspA antibody values in <jats:italic>B burgdorferi<\/jats:italic>\u2013positive NB cases (n = 13) were significantly elevated (<jats:italic>P<\/jats:italic> &lt; .001) compared to controls (15), and OspA was the predominant antigen detected by ELISA (8). Histopathology did not vary between NB cases with (n = 9) and without (6) <jats:italic>B burgdorferi<\/jats:italic> PCR detection.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>CONCLUSIONS<\/jats:title>\n<jats:p>The presence of <jats:italic>B burgdorferi<\/jats:italic> in the nuchal bursa of horses is associated with increased serum OspA antibodies.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>CLINICAL RELEVANCE<\/jats:title>\n<jats:p>The role of <jats:italic>B burgdorferi<\/jats:italic> in equine NB may be underestimated, and targeted therapy requires investigation.<\/jats:p>\n<\/jats:sec>","DOI":"10.2460\/javma.24.05.0312","type":"journal-article","created":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T14:05:45Z","timestamp":1725631545000},"page":"1476-1484","source":"Crossref","is-referenced-by-count":7,"title":["Association of Borrelia burgdorferi with nuchal bursitis and elevated outer surface protein A\u2013specific serum antibodies in horses of the northeastern United States"],"prefix":"10.2460","volume":"262","author":[{"given":"Erin K.","family":"Pearson","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cassandra","family":"Guarino","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Cercone","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Divers","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenna","family":"Lambert","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Garc\u00eda-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, PA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy L.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, PA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie B.","family":"Engiles","sequence":"additional","affiliation":[{"name":"Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, PA"},{"name":"Department of Pathobiology, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, PA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Marconi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medicine, Virginia Commonwealth University, Richmond, VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jade","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medicine, Virginia Commonwealth University, Richmond, VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kara","family":"Brown","sequence":"additional","affiliation":[{"name":"Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, PA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toby","family":"Pinn-Woodcock","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"issue":"7","key":"ref1","series-title":"J Am Vet Med Assoc","first-page":"823","article-title":"Diagnosis and management of cranial and caudal nuchal bursitis in four horses","volume":"237","author":"Garc\u00eda-L\u00f3pez JM","year":"2010","unstructured":"Garc\u00eda-L\u00f3pez JM, Jenei T, Chope K, Bubeck KA. Diagnosis and management of cranial and caudal nuchal bursitis in four horses. J Am Vet Med Assoc. 2010;237(7):823-829. doi:10.2460\/javma.237.7.823"},{"issue":"4","key":"ref2","series-title":"Equine Vet J","first-page":"465","article-title":"Diagnosis, treatment and outcome of cranial nuchal bursitis in 30 horses","volume":"50","author":"Bergren AL","year":"2018","unstructured":"Bergren AL, Abuja GA, Bubeck KA, Spoormakers TJP, Garc\u00eda-L\u00f3pez JM. Diagnosis, treatment and outcome of cranial nuchal bursitis in 30 horses. Equine Vet J. 2018;50(4):465-469. doi:10.1111\/evj.12787"},{"issue":"3","key":"ref3","series-title":"Equine Vet J","first-page":"121","article-title":"A review of brucellosis in the horse","volume":"5","author":"Denny HR","year":"1973","unstructured":"Denny HR. A review of brucellosis in the horse. Equine Vet J. 1973;5(3):121-125. doi:10.1111\/j.2042-3306.1973.tb03208.x"},{"issue":"2","key":"ref4","series-title":"J Vet Intern Med","first-page":"617","article-title":"Borrelia burgdorferi infection and Lyme disease in North American horses: a consensus statement","volume":"32","author":"Divers TJ","year":"2018","unstructured":"Divers TJ, Gardner RB, Madigan JE,  Borrelia burgdorferi infection and Lyme disease in North American horses: a consensus statement. J Vet Intern Med. 2018;32(2):617-632. doi:10.1111\/jvim.15042"},{"key":"ref5","series-title":"Front Vet Sci","first-page":"743067","article-title":"Case report: nuchal bursitis associated with Borrelia burgdorferi infection in a horse","volume":"8","author":"Guarino C","year":"2021","unstructured":"Guarino C, Pinn-Woodcock T, Levine DG, Miller J, Johnson AL. Case report: nuchal bursitis associated with Borrelia burgdorferi infection in a horse. Front Vet Sci. 2021;8:743067. doi:10.3389\/fvets.2021.743067"},{"issue":"4","key":"ref6","series-title":"Clin Vaccine Immunol","first-page":"527","article-title":"Antibodies to Borrelia burgdorferi OspA, OspC, OspF, and C6 antigens as markers for early and late infection in dogs","volume":"19","author":"Wagner B","year":"2012","unstructured":"Wagner B, Freer H, Rollins A,  Antibodies to Borrelia burgdorferi OspA, OspC, OspF, and C6 antigens as markers for early and late infection in dogs. Clin Vaccine Immunol. 2012;19(4):527-535. doi:10.1128\/CVI.05653-11"},{"issue":"6","key":"ref7","series-title":"J Clin Microbiol","first-page":"1958","article-title":"Quantitative detection of Borrelia burgdorferi by real-time PCR","volume":"37","author":"Pahl A","year":"1999","unstructured":"Pahl A, K\u00fchlbrandt U, Brune K, R\u00f6llinghoff M, Gessner A. Quantitative detection of Borrelia burgdorferi by real-time PCR. J Clin Microbiol. 1999;37(6):1958-1963. doi:10.1128\/JCM.37.6.1958-1963.1999"},{"issue":"3-4","key":"ref8","series-title":"Vet Immunol Immunopathol","first-page":"374","article-title":"Development of a multiplex assay for the detection of antibodies to Borrelia burgdorferi in horses and its validation using Bayesian and conventional statistical methods","volume":"144","author":"Wagner B","year":"2011","unstructured":"Wagner B, Freer H, Rollins A, Erb HN, Lu Z, Gr\u00f6hn Y. Development of a multiplex assay for the detection of antibodies to Borrelia burgdorferi in horses and its validation using Bayesian and conventional statistical methods. Vet Immunol Immunopathol. 2011;144(3-4):374-381. doi:10.1016\/j.vetimm.2011.08.005"},{"issue":"3","key":"ref9","series-title":"Infect Immun","first-page":"e0052923","article-title":"Borreliella burgdorferi factor H-binding proteins are not required for serum resistance and infection in mammals","volume":"92","author":"Cramer NA","year":"2024","unstructured":"Cramer NA, Socarras KM, Earl J, Ehrlich GD, Marconi RT. Borreliella burgdorferi factor H-binding proteins are not required for serum resistance and infection in mammals. Infect Immun. 2024;92(3):e0052923. doi:10.1128\/iai.00529-23"},{"issue":"16","key":"ref10","series-title":"J Biol Chem","first-page":"12715","article-title":"Structure of factor H-binding protein B (FhbB) of the periopathogen, Treponema denticola: insights into progression of periodontal disease","volume":"287","author":"Miller DP","year":"2012","unstructured":"Miller DP, Bell JK, McDowell JV,  Structure of factor H-binding protein B (FhbB) of the periopathogen, Treponema denticola: insights into progression of periodontal disease. J Biol Chem. 2012;287(16):12715-12722. doi:10.1074\/jbc.M112.339721"},{"key":"ref11","series-title":"Vaccine X","first-page":"100382","article-title":"The leptospiral OmpA-like protein (Loa22) is a surface-exposed antigen that elicits bactericidal antibody against heterologous Leptospira","volume":"15","author":"Schuler EJA","year":"2023","unstructured":"Schuler EJA, Patel DT, Marconi RT. The leptospiral OmpA-like protein (Loa22) is a surface-exposed antigen that elicits bactericidal antibody against heterologous Leptospira. Vaccine X. 2023;15:100382. doi:10.1016\/j.jvacx.2023.100382"},{"issue":"10","key":"ref12","series-title":"Infect Immun","first-page":"e0036422","article-title":"FtlA and FtlB are candidates for inclusion in a next-generation multiantigen subunit vaccine for Lyme disease","volume":"90","author":"Camire AC","year":"2022","unstructured":"Camire AC, O\u2019Bier NS, Patel DT,  FtlA and FtlB are candidates for inclusion in a next-generation multiantigen subunit vaccine for Lyme disease. Infect Immun. 2022;90(10):e0036422. doi:10.1128\/iai.00364-22"},{"issue":"33","key":"ref13","series-title":"Vaccine","first-page":"4140","article-title":"Vaccination of horses with Lyme vaccines for dogs induces short-lasting antibody responses","volume":"35","author":"Guarino C","year":"2017","unstructured":"Guarino C, Asbie S, Rohde J, Glaser A, Wagner B. Vaccination of horses with Lyme vaccines for dogs induces short-lasting antibody responses. Vaccine. 2017;35(33):4140-4147. doi:10.1016\/j.vaccine.2017.06.052"},{"issue":"1","key":"ref14","series-title":"Infect Immun","first-page":"173","article-title":"The immunoglobulin (IgG) antibody response to OspA and OspB correlates with severe and prolonged Lyme arthritis and the IgG response to P35 correlates with mild and brief arthritis","volume":"67","author":"Akin E","year":"1999","unstructured":"Akin E, McHugh GL, Flavell RA, Fikrig E, Steere AC. The immunoglobulin (IgG) antibody response to OspA and OspB correlates with severe and prolonged Lyme arthritis and the IgG response to P35 correlates with mild and brief arthritis. Infect Immun. 1999;67(1):173-181. doi:10.1128\/IAI.67.1.173-181.1999"},{"issue":"7","key":"ref15","series-title":"Infect Immun","first-page":"2774","article-title":"Association of treatment-resistant chronic Lyme arthritis with HLA-DR4 and antibody reactivity to OspA and OspB of Borrelia burgdorferi","volume":"61","author":"Kalish RA","year":"1993","unstructured":"Kalish RA, Leong JM, Steere AC. Association of treatment-resistant chronic Lyme arthritis with HLA-DR4 and antibody reactivity to OspA and OspB of Borrelia burgdorferi. Infect Immun. 1993;61(7):2774-2779. doi:10.1128\/iai.61.7.2774-2779.1993"},{"issue":"1","key":"ref16","series-title":"J Clin Microbiol","first-page":"382","article-title":"Temporal changes in outer surface proteins A and C of the Lyme disease-associated spirochete, Borrelia burgdorferi, during the chain of infection in ticks and mice","volume":"38","author":"Schwan TG","year":"2000","unstructured":"Schwan TG, Piesman J. Temporal changes in outer surface proteins A and C of the Lyme disease-associated spirochete, Borrelia burgdorferi, during the chain of infection in ticks and mice. J Clin Microbiol. 2000;38(1):382-388. doi:10.1128\/JCM.38.1.382-388.2000"},{"issue":"4","key":"ref17","series-title":"J Vet Intern Med","first-page":"1300","article-title":"Seroprevalence of Borrelia burgdorferi in horses presented for Coggins testing in southwest Virginia and change in positive test results approximately 1 year later","volume":"30","author":"Funk RA","year":"2016","unstructured":"Funk RA, Pleasant RS, Witonsky SG, Reeder DS, Werre SR, Hodgson DR. Seroprevalence of Borrelia burgdorferi in horses presented for Coggins testing in southwest Virginia and change in positive test results approximately 1 year later. J Vet Intern Med. 2016;30(4):1300-1304. doi:10.1111\/jvim.13973"},{"issue":"4","key":"ref18","series-title":"J Vet Diagn Invest","first-page":"736","article-title":"Evaluation of 2 ELISAs to determine Borrelia burgdorferi seropositivity in horses over a 12-month period","volume":"33","author":"Neely M","year":"2021","unstructured":"Neely M, Arroyo L, Jardine C,  Evaluation of 2 ELISAs to determine Borrelia burgdorferi seropositivity in horses over a 12-month period. J Vet Diagn Invest. 2021;33(4):736-739. doi:10.1177\/10406387211016103"},{"issue":"2","key":"ref19","series-title":"Equine Vet J","first-page":"331","article-title":"Seroprevalence and evaluation of risk factors associated with seropositivity for Borrelia burgdorferi in Ontario horses","volume":"53","author":"Neely M","year":"2021","unstructured":"Neely M, Arroyo LG, Jardine C,  Seroprevalence and evaluation of risk factors associated with seropositivity for Borrelia burgdorferi in Ontario horses. Equine Vet J. 2021;53(2):331-338. doi:10.1111\/evj.13317"},{"issue":"10","key":"ref20","series-title":"Equine Vet Educ","first-page":"572","article-title":"Humoral response of Borrelia burgdorferi outer surface protein A (OspA) vaccination in equids","volume":"29","author":"Slaughter KM","year":"2017","unstructured":"Slaughter KM, Halland SK, Schur LA, Wagner B, DeMel DM, Bertone JJ. Humoral response of Borrelia burgdorferi outer surface protein A (OspA) vaccination in equids. Equine Vet Educ. 2017;29(10):572-576. doi:10.1111\/eve.12690"},{"issue":"3","key":"ref21","series-title":"J Infect Dis","first-page":"651","article-title":"Experimental Lyme disease in dogs produces arthritis and persistent infection","volume":"167","author":"Appel MJG","year":"1993","unstructured":"Appel MJG, Allan S, Jacobson RH,  Experimental Lyme disease in dogs produces arthritis and persistent infection. J Infect Dis. 1993;167(3):651-664. doi:10.1093\/infdis\/167.3.651"},{"key":"ref22","series-title":"Curr Issues Mol Biol","first-page":"145","article-title":"Immune response to Borrelia: lessons from Lyme disease spirochetes","volume":"42","author":"Bockenstedt LK","year":"2021","unstructured":"Bockenstedt LK, Wooten RM, Baumgarth N. Immune response to Borrelia: lessons from Lyme disease spirochetes. Curr Issues Mol Biol. 2021;42:145-190. doi:10.21775\/cimb.042.145"},{"issue":"1","key":"ref23","series-title":"Vet Pathol","first-page":"68","article-title":"Experimental infection of ponies with Borrelia burgdorferi by exposure to Ixodid ticks","volume":"37","author":"Chang YF","year":"2000","unstructured":"Chang YF, Novosol V, McDonough SP,  Experimental infection of ponies with Borrelia burgdorferi by exposure to Ixodid ticks. Vet Pathol. 2000;37(1):68-76. doi:10.1354\/vp.37-1-68"},{"issue":"2","key":"ref24","series-title":"Vet Clin North Am Equine Pract","first-page":"353","article-title":"Head tilt in horses","volume":"3","author":"Watrous BJ","year":"1987","unstructured":"Watrous BJ. Head tilt in horses. Vet Clin North Am Equine Pract. 1987;3(2):353-370. doi:10.1016\/S0749-0739(17)30678-8"},{"issue":"4","key":"ref25","series-title":"Can Vet J","first-page":"382","article-title":"Ceratohyoidectomy for treatment of equine temporohyoid osteoarthopathy (15 cases)","volume":"56","author":"Oliver ST","year":"2015","unstructured":"Oliver ST, Hardy J. Ceratohyoidectomy for treatment of equine temporohyoid osteoarthopathy (15 cases). Can Vet J. 2015;56(4):382-386."},{"issue":"3","key":"ref26","series-title":"Am J Pathol","first-page":"959","article-title":"Chronic Lyme borreliosis in the laboratory mouse","volume":"143","author":"Barthold SW","year":"1993","unstructured":"Barthold SW, de Souza MS, Janotka JL, Smith AL, Persing DH. Chronic Lyme borreliosis in the laboratory mouse. Am J Pathol. 1993;143(3):959-971."},{"issue":"4","key":"ref27","series-title":"J Vet Intern Med","first-page":"1305","article-title":"Retrospective evaluation of horses diagnosed with neuroborreliosis on postmortem examination: 16 cases (2004-2015)","volume":"30","author":"Johnstone LK","year":"2016","unstructured":"Johnstone LK, Engiles JB, Aceto H,  Retrospective evaluation of horses diagnosed with neuroborreliosis on postmortem examination: 16 cases (2004-2015). J Vet Intern Med. 2016;30(4):1305-1312. doi:10.1111\/jvim.14369"},{"issue":"6","key":"ref28","series-title":"Vet Ophthalmol","first-page":"398","article-title":"Diagnosis of Borrelia-associated uveitis in two horses","volume":"15","author":"Priest HL","year":"2012","unstructured":"Priest HL, Irby NL, Schlafer DH,  Diagnosis of Borrelia-associated uveitis in two horses. Vet Ophthalmol. 2012;15(6):398-405. doi:10.1111\/j.1463-5224.2012.01000.x"},{"key":"ref29","series-title":"Abstract presented at: Cornell University College of Veterinary Medicine\u2019s Clinical Investigators\u2019 Day","article-title":"Post-mortem characterization of subclinical inflammation of nuchal bursae and nuchal ligaments in horses","author":"Sfraga HRM","unstructured":"Sfraga HRM, Demeter EA, Pinn-Woodcock TL, Guarino C, Cercone M. Post-mortem characterization of subclinical inflammation of nuchal bursae and nuchal ligaments in horses. Abstract presented at: Cornell University College of Veterinary Medicine\u2019s Clinical Investigators\u2019 Day; March 15, 2024; Ithaca, NY."},{"issue":"6","key":"ref30","series-title":"Vet Pathol","first-page":"1151","article-title":"Lyme neuroborreliosis in 2 horses","volume":"48","author":"Imai DM","year":"2011","unstructured":"Imai DM, Barr BC, Daft B,  Lyme neuroborreliosis in 2 horses. Vet Pathol. 2011;48(6):1151-1157. doi:10.1177\/0300985811398246"},{"issue":"6","key":"ref31","series-title":"Vet Ophthalmol","first-page":"1014","article-title":"Ocular disease in horses with confirmed ocular or central nervous system Borrelia infection: case series and review of literature","volume":"23","author":"Scherrer NM","year":"2020","unstructured":"Scherrer NM, Knickelbein KE, Engiles JB, Johnstone LK, Tewari D, Johnson AL. Ocular disease in horses with confirmed ocular or central nervous system Borrelia infection: case series and review of literature. Vet Ophthalmol. 2020;23(6):1014-1024. doi:10.1111\/vop.12817"},{"issue":"9","key":"ref32","series-title":"Arthritis Rheumatol","first-page":"2313","article-title":"Review: unraveling Lyme disease","volume":"66","author":"Bockenstedt LK","year":"2014","unstructured":"Bockenstedt LK, Wormser GP. Review: unraveling Lyme disease. Arthritis Rheumatol. 2014;66(9):2313-2323. doi:10.1002\/art.38756"},{"issue":"7","key":"ref33","series-title":"Am J Vet Res","first-page":"1104","article-title":"Experimental induction of chronic borreliosis in adult dogs exposed to Borrelia burgdorferi-infected ticks and treated with dexamethasone","volume":"62","author":"Chang YF","year":"2001","unstructured":"Chang YF, Novosel V, Chang CF,  Experimental induction of chronic borreliosis in adult dogs exposed to Borrelia burgdorferi-infected ticks and treated with dexamethasone. Am J Vet Res. 2001;62(7):1104-1112. doi:10.2460\/ajvr.2001.62.1104"},{"issue":"3","key":"ref34","series-title":"Brain Pathol","first-page":"297","article-title":"Effects of formalin fixation, paraffin embedding, and time of storage on DNA preservation in brain tissue: a BrainNet Europe study","volume":"17","author":"Ferrer I","year":"2007","unstructured":"Ferrer I, Armstrong J, Capellari S,  Effects of formalin fixation, paraffin embedding, and time of storage on DNA preservation in brain tissue: a BrainNet Europe study. Brain Pathol. 2007;17(3):297-303. doi:10.1111\/j.1750-3639.2007.00073.x"},{"key":"ref35","article-title":"USDA APHIS","unstructured":"National Brucellosis Eradication Program. USDA APHIS. June 7, 2023. Updated June 21, 2024. Accessed December 8, 2023. https:\/\/www.aphis.usda.gov\/aphis\/ourfocus\/animalhealth\/animal-disease-information\/cattle-disease-information\/national-brucellosis-eradication\/brucellosis-eradication-program"}],"container-title":["Journal of the American Veterinary Medical Association"],"original-title":[],"link":[{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/javma\/262\/11\/javma.24.05.0312.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/javma\/262\/11\/javma.24.05.0312.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/javma\/262\/11\/javma.24.05.0312.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T13:09:31Z","timestamp":1741352971000},"score":1,"resource":{"primary":{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/javma\/262\/11\/javma.24.05.0312.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,1]]},"references-count":35,"journal-issue":{"issue":"11"},"URL":"https:\/\/doi.org\/10.2460\/javma.24.05.0312","relation":{},"ISSN":["0003-1488"],"issn-type":[{"value":"0003-1488","type":"print"}],"subject":[],"published":{"date-parts":[[2024,11,1]]}}}