{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T06:52:19Z","timestamp":1787986339719,"version":"build-2784847793"},"reference-count":38,"publisher":"American Veterinary Medical Association (AVMA)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["javma"],"published-print":{"date-parts":[[2012,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Case Description<\/jats:bold>\u2014An underweight, lethargic adult female California sea lion (<jats:italic>Zalophus californianus<\/jats:italic>) became stranded along the California shore and was captured and transported to a rehabilitation hospital for assessment and care.<\/jats:p>\n<jats:p><jats:bold>Clinical Findings<\/jats:bold>\u2014Initial physical assessment revealed the sea lion was lethargic and in poor body condition. Active myositis was diagnosed on the basis of concurrent elevations in activities of alanine aminotransferase and creatine kinase detected during serum biochemical analysis. Infection with <jats:italic>Sarcocystis neurona<\/jats:italic> was diagnosed after serologic titers increased 4-fold over a 3-week period. Diagnosis was confirmed on the basis of histopathologic findings, positive results on immunohistochemical staining, and results of quantitative PCR assay on biopsy specimens obtained from the diaphragm and muscles of the dorsal cervical region.<\/jats:p>\n<jats:p><jats:bold>Treatment and Outcome<\/jats:bold>\u2014Anticoccidial treatment was instituted with ponazuril (10 mg\/kg [4.5 mg\/lb], PO, q 24 h) and continued for 28 days. Prednisone (0.2 mg\/kg [0.09 mg\/lb], PO, q 12 h) was administered for 2 days and then every 24 hours for 5 days to treat associated inflammation. At the end of treatment, the sea lion was clinically normal, alanine aminotransferase and creatine kinase values were within reference limits, and antibody titers against <jats:italic>S neurona<\/jats:italic> had decreased 6-fold. The sea lion was released approximately 3 months after becoming stranded.<\/jats:p>\n<jats:p><jats:bold>Clinical Relevance<\/jats:bold>\u2014<jats:italic>S neurona<\/jats:italic>\u2013induced myositis was diagnosed in a free-ranging California sea lion. On the basis of the successful treatment and release of this sea lion, anticoccidial treatment should be considered for marine mammals in which protozoal disease is diagnosed.<\/jats:p>","DOI":"10.2460\/javma.240.3.324","type":"journal-article","created":{"date-parts":[[2012,1,18]],"date-time":"2012-01-18T18:06:12Z","timestamp":1326909972000},"page":"324-328","source":"Crossref","is-referenced-by-count":23,"title":["Diagnosis and treatment of Sarcocystis neurona\u2013induced myositis in a free-ranging California sea lion"],"prefix":"10.2460","volume":"240","author":[{"given":"Daphne P.","family":"Carlson-Bremer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frances M. 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pathology","author":"Bossart","year":"2001"},{"key":"ref3","series-title":"J Comp Pathol","first-page":"607","article-title":"The tissue activities of some diagnostic enzymes in ten mammalian species","volume":"88","author":"Clampitt","year":"1978"},{"key":"ref4","series-title":"J Zoo Wildl Med","first-page":"1","article-title":"Distribution of tissue enzymes in three species of pinnipeds","volume":"39","author":"Fauquier","year":"2008"},{"key":"ref5","series-title":"Zoo and wildlife medicine","first-page":"604","article-title":"Clinical pathology of marine mammals","author":"Medway","year":"1978"},{"key":"ref6","series-title":"Diseases of marine mammals","first-page":"683","article-title":"Diseases of mammalia: pinnipedia","volume":"4","author":"Lauckner","year":"1985"},{"key":"ref7","series-title":"J Wildl Dis","first-page":"423","article-title":"Findings in pinnipeds stranded along the central and northern California coast, 1984\u20131990","volume":"29","author":"Gerber","year":"1993"},{"key":"ref8","series-title":"J Zoo Wildl Med","first-page":"41","article-title":"Rehabilitation and treatment success rate of California sea lions (Zalophus californianus) and northern fur seals (Callorhinus ursinus) stranded along the central and northern California coast, 1984\u20131990","volume":"24","author":"Gage","year":"1993"},{"key":"ref9","series-title":"Small animal clinical diagnosis by laboratory methods","first-page":"322","article-title":"Neurologic disorders","author":"Parent","year":"2004"},{"key":"ref10","series-title":"J Wildl Dis","first-page":"40","article-title":"Lesions associated with a novel Mycoplasma sp. in California sea lions (Zalophus californianus) undergoing rehabilitation","volume":"42","author":"Haulena","year":"2006"},{"key":"ref11","series-title":"Zoo and wildlife medicine","first-page":"319","article-title":"Tissue-cyst forming coccidian of marine mammals","volume":"6","author":"Miller","year":"2008"},{"key":"ref12","series-title":"J Vet Diagn Invest","first-page":"486","article-title":"Acute hepatic necrosis associated with a Sarcocystis-like protozoa in a sea lion (Zalophus californianus)","volume":"4","author":"Mense","year":"1992"},{"key":"ref13","series-title":"Vet Parasitol","first-page":"275","article-title":"Toxoplasma gondii, Neospora caninum, Sarcocystis neurona and Sarcocystis canis-like infections in marine mammals","volume":"116","author":"Dubey","year":"2003"},{"key":"ref14","series-title":"J Wildl Dis","first-page":"53","article-title":"Sarcocystis in the northern fur seal","volume":"10","author":"Brown","year":"1974"},{"key":"ref15","series-title":"J Wildl Dis","first-page":"291","article-title":"Prevalence of Sarcocystis sp. in stranded Atlantic white-sided dolphins (Lagenorhynchus acutus)","volume":"38","author":"Ewing","year":"2002"},{"key":"ref16","series-title":"J Protozool","first-page":"290","article-title":"A new species of Sarcocystis parasite in the whale Balaenoptera borealis","volume":"17","author":"Akao","year":"1970"},{"key":"ref17","series-title":"J Wildl Dis","first-page":"285","article-title":"Parasite-related lesions in a bearded seal, Erignathus barbatus","volume":"15","author":"Bishop","year":"1979"},{"key":"ref18","series-title":"J Wildl Dis","first-page":"503","article-title":"Parasites and associated pathology observed in cetaceans stranded along the Oregon coast","volume":"14","author":"Dailey","year":"1978"},{"key":"ref19","series-title":"J Vet Diagn Invest","first-page":"296","article-title":"Intramuscular Sarcocystis in two beluga whales and an Atlantic white-sided dolphin from the St. Lawrence estuary, Quebec, Canada","volume":"5","author":"DeGuise","year":"1993"},{"key":"ref20","series-title":"J Parasitol","first-page":"397","article-title":"Sarcocysts of an unidentified species of Sarcocystis in the sea otter (Enhydra lutris)","volume":"89","author":"Dubey","year":"2003"},{"key":"ref21","series-title":"J Am Vet Med Assoc","first-page":"917","article-title":"Sarcosporidiosis in the ringed seal","volume":"177","author":"Migaki","year":"1980"},{"key":"ref22","series-title":"Vet Parasitol","first-page":"19","article-title":"Naturally occurring Sarcocystis neurona-like infection in a dog with myositis","volume":"133","author":"Vashisht","year":"2005"},{"key":"ref23","series-title":"J Am Vet Med Assoc","first-page":"1574","article-title":"Multifocal myositis associated with Sarcocystis sp in a horse","volume":"205","author":"Traub-Dargatz","year":"1994"},{"key":"ref24","series-title":"J Parasitol","first-page":"187","article-title":"Clinical muscular sarcocystosis in a dog","volume":"91","author":"Chapman","year":"2005"},{"key":"ref25","series-title":"Am J Trop Med Hyg","first-page":"548","article-title":"An outbreak of acute eosinophilic myositis attributed to human Sarcocystis parasitism","volume":"61","author":"Arness","year":"1999"},{"key":"ref26","series-title":"Sarcocystis of animals and man","first-page":"1","author":"Dubey","year":"1989"},{"key":"ref27","series-title":"J Comp Pathol","first-page":"249","article-title":"Eosinophilic myositis due to Sarcocystis hominis in a beef cow","volume":"135","author":"Woulda","year":"2006"},{"key":"ref28","series-title":"Vet Ophthalmol","first-page":"259","article-title":"Equine protozoal myeloencephalitis due to Neospora hughesi and equine motor neuron disease in a mule","volume":"13","author":"Finno","year":"2000"},{"key":"ref29","series-title":"Vet Parasitol","first-page":"89","article-title":"A review of Sarcocystis neurona and equine protozoal myeloencephalitis (EPM)","volume":"95","author":"Dubey","year":"2001"},{"key":"ref30","series-title":"J Parasitol","first-page":"639","article-title":"Efficacy of ponazuril in vitro and in preventing and treating Toxoplasma gondii infections in mice","volume":"90","author":"Mitchell","year":"2004"},{"key":"ref31","series-title":"J Zoo Wildl Med","first-page":"228","article-title":"Diagnosis and treatment of Sarcocysitis neurona in a captive harbor seal (Phoca vitulina)","volume":"39","author":"Mylniczenko","year":"2008"},{"key":"ref32","series-title":"J Parasitol","first-page":"1184","article-title":"Meningoencephalitis due to a Sarcocystis neurona-like protozoan in Pacific harbor seals (Phoca vitulina richardsi)","volume":"84","author":"Lapointe","year":"1998"},{"key":"ref33","series-title":"J Parasitol","first-page":"816","article-title":"Isolation and characterization of two parasitic protozoa from a Pacific harbor seal (Phoca vitulina richardsi) with meningoencephalitis","volume":"87","author":"Miller","year":"2001"},{"key":"ref34","series-title":"Int J Parasitol","first-page":"1155","article-title":"Transmssion of Toxoplasma: clues from the study of sea otters as sentinels of Toxoplasma gondii flow into the marine environment","volume":"35","author":"Conrad","year":"2005"},{"key":"ref35","series-title":"Int J Parasitol","first-page":"997","article-title":"Coastal freshwater runoff is a risk factor for Toxoplasma gondii infection of southern sea otters (Enhydra lutris nereis)","volume":"32","author":"Miller","year":"2002"},{"key":"ref36","series-title":"Int J Parasitol","first-page":"1251","article-title":"Toxoplasma gondii: from animals to humans","volume":"30","author":"Tenter","year":"2000"},{"key":"ref37","series-title":"J Parasitol","first-page":"197","article-title":"Congenital toxoplasmosis in an Indo-Pacific bottlenose dolphin (Tursiops aduncus)","volume":"88","author":"Jardine","year":"2002"},{"key":"ref38","series-title":"J Parasitol","first-page":"1029","article-title":"Disseminated toxoplasmosis in a Mediterranean pregnant Risso's dolphin (Grampus griseus) with transplacental fetal infection","volume":"88","author":"Resendes","year":"2002"}],"container-title":["Journal of the American 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