{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T21:40:00Z","timestamp":1784756400117,"version":"3.55.0"},"reference-count":12,"publisher":"American Veterinary Medical Association (AVMA)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["javma"],"published-print":{"date-parts":[[2012,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To compare 2 assays for use in the identification of dogs with a protective antibody titer (PAT) against canine parvovirus (CPV) and canine distemper virus (CDV).<\/jats:p>\n<jats:p><jats:bold>Design<\/jats:bold>\u2014Prospective cross-sectional study.<\/jats:p>\n<jats:p><jats:bold>Animals<\/jats:bold>\u2014431 dogs admitted to a municipal animal shelter in north central Florida.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014Blood samples were collected from dogs on the day of admission to the shelter. Serum was obtained, criterion-referenced assays were used to identify dogs that had PATs against CPV (titers \u2265 80; hemagglutination inhibition assay) and CDV (titers \u2265 32; virus neutralization assay), and results were compared with results of a semiquantitative ELISA and an immunofluorescence assay (IFA).<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014For correct identification of dogs that had PATs against viruses, the ELISA had significantly higher specificity for CPV (98%) and CDV (95%) than did the IFA (82% and 70%, respectively) and had significantly lower sensitivity for CDV (88%) than did the IFA (97%); the sensitivity for CPV was similar (ELISA, 98%; IFA, 97%). Overall diagnostic accuracy was significantly greater with the ELISA than with the IFA. Predictive value of a positive result for PATs was significantly higher with the ELISA for CPV (99%) and CDV (93%) than with the IFA (92% and 71 %, respectively).<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014The ELISA had fewer false-positive results than did the IFA and could be performed on-site in shelters in &lt; 1 hour. Accuracy and practicality of the ELISA may be useful for identifying the infection risk of dogs exposed during outbreaks attributable to CPV and CDV infections in shelters.<\/jats:p>","DOI":"10.2460\/javma.240.9.1084","type":"journal-article","created":{"date-parts":[[2012,4,19]],"date-time":"2012-04-19T14:39:55Z","timestamp":1334846395000},"page":"1084-1087","source":"Crossref","is-referenced-by-count":34,"title":["Comparison of two assays for detection of antibodies against canine parvovirus and canine distemper virus in dogs admitted to a Florida animal shelter"],"prefix":"10.2460","volume":"240","author":[{"given":"Lauren K.","family":"Gray","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. Cynda","family":"Crawford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie K.","family":"Levy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward J.","family":"Dubovi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"J Am Vet Med Assoc","first-page":"1317","article-title":"Prevalence of protective antibody titers for canine distemper virus and canine parvovirus in dogs entering a Florida animal shelter","volume":"236","author":"Lechner","year":"2010"},{"key":"ref2","series-title":"Infectious disease management in animal shelters","first-page":"39","article-title":"Outbreak management","author":"Hurley","year":"2009"},{"key":"ref3","series-title":"J Am Vet Med Assoc","first-page":"72","article-title":"Serum distemper virus and parvovirus antibody titers among dogs brought to a veterinary hospital for revaccination","volume":"213","author":"McCaw","year":"1998"},{"key":"ref4","series-title":"J Am Vet Med Assoc","first-page":"1021","article-title":"Clinical use of serum parvovirus and distemper virus antibody titers for determining revaccination strategies in healthy dogs","volume":"217","author":"Twark","year":"2000"},{"key":"ref5","series-title":"J Am Vet Med Assoc","first-page":"200","article-title":"A perspective on vaccine guidelines and titer tests for dogs","volume":"224","author":"Moore","year":"2004"},{"key":"ref6","series-title":"Vet Microbiol","first-page":"2","article-title":"Vaccine use and disease prevalence in dogs and cats","volume":"117","author":"Horzinek","year":"2006"},{"key":"ref7","series-title":"Vet Microbiol","first-page":"39","article-title":"Serological testing\u2014an alternative to boosters?","volume":"117","author":"Burr","year":"2006"},{"key":"ref8","series-title":"Am J Vet Res","first-page":"784","article-title":"Hemagglutination by canine parvovirus: serologic studies and diagnostic applications","volume":"41","author":"Carmichael","year":"1980"},{"key":"ref9","series-title":"J Am Vet Med Assoc","first-page":"37","article-title":"Maternally derived immunity to canine parvovirus infection: transfer, decline, and interference with vaccination","volume":"180","author":"Pollack","year":"1982"},{"key":"ref10","series-title":"Am J Vet Res","first-page":"1459","article-title":"A microneutralization test for canine distemper virus","volume":"34","author":"Appel","year":"1973"},{"key":"ref11","series-title":"Canine distemper-parvovirus antibody test kit [package insert]","year":"2010"},{"key":"ref12","series-title":"Infectious disease management in animal shelters","first-page":"61","article-title":"Canine and feline vaccinations and immunology","author":"Larson","year":"2009"}],"container-title":["Journal of the American Veterinary Medical Association"],"original-title":[],"link":[{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/javma\/240\/9\/javma.240.9.1084.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/javma\/240\/9\/javma.240.9.1084.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/javma\/240\/9\/javma.240.9.1084.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T13:11:13Z","timestamp":1741353073000},"score":1,"resource":{"primary":{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/javma\/240\/9\/javma.240.9.1084.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,5,1]]},"references-count":12,"journal-issue":{"issue":"9"},"URL":"https:\/\/doi.org\/10.2460\/javma.240.9.1084","relation":{},"ISSN":["0003-1488"],"issn-type":[{"value":"0003-1488","type":"print"}],"subject":[],"published":{"date-parts":[[2012,5,1]]}}}