{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T02:01:48Z","timestamp":1775872908132,"version":"3.50.1"},"reference-count":46,"publisher":"American Veterinary Medical Association (AVMA)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["javma"],"published-print":{"date-parts":[[2013,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To evaluate the effect of IV administration of polymyxin B on clinical and serum biochemical variables in foals with experimental endotoxemia.<\/jats:p>\n<jats:p><jats:bold>Design<\/jats:bold>\u2014Prospective experimental study.<\/jats:p>\n<jats:p><jats:bold>Animals<\/jats:bold>\u201414 healthy neonatal foals.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014Foals were randomly assigned to a treatment or control group and were administered a single dose of lipopolysaccharide (0.5 \u03bcg\/kg [0.23 \u03bcg\/lb]) IV over 30 minutes. The treatment group received polymyxin B (6,000 U\/kg [2,727 U\/lb], IV) immediately after completion of lipopolysaccharide infusion; the control group was administered an equal volume of saline (0.9% NaCl) solution. Subsequent doses of polymyxin B or saline solution were administered IV at 8 and 16 hours. Blood was collected at various time points, and outcome variables, including heart rate, respiratory rate, rectal temperature, attitude score, WBC count, neutrophil count, lymphocyte count, monocyte count, platelet count, Hct, blood lactate concentration, blood glucose concentration, serum tumor necrosis factor-\u03b1 concentration, and plasma thromboxane B<jats:sub>2<\/jats:sub> concentration, were measured. Urine was collected prior to and after experimentation to determine whether nephrotoxicosis was associated with treatment.<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014The treatment group had significantly lower blood lactate concentration and serum tumor necrosis factor-\u03b1 and plasma thromboxane B<jats:sub>2<\/jats:sub> concentrations and had higher blood glucose concentrations and better attitude scores, compared with the control group, at various time points during the study. No other significant differences and no evidence of overt nephrotoxicosis were detected.<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Administration of polymyxin B IV in healthy neonatal foals challenged with lipopolysaccharide attenuated some clinical and serum biochemical derangements associated with endotoxemia.<\/jats:p>","DOI":"10.2460\/javma.243.6.874","type":"journal-article","created":{"date-parts":[[2013,9,4]],"date-time":"2013-09-04T14:44:13Z","timestamp":1378305853000},"page":"874-881","source":"Crossref","is-referenced-by-count":24,"title":["Effects of intravenous administration of polymyxin B in neonatal foals with experimental endotoxemia"],"prefix":"10.2460","volume":"243","author":[{"given":"David M.","family":"Wong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brett 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Med","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1111\/j.1939-1676.1998.tb02109.x","article-title":"Factors associated with survival in septicemic foals: 65 cases (1988\u20131995)","volume":"12","author":"Gayle","year":"1998"},{"key":"ref3","series-title":"J Am Vet Med Assoc","doi-asserted-by":"publisher","first-page":"1608","DOI":"10.2460\/javma.2001.218.1608","article-title":"Bacterial isolates from blood and their susceptibility patterns in critically ill foals: 543 cases (1991\u20131998)","volume":"218","author":"Marsh","year":"2001"},{"key":"ref4","series-title":"J Am Vet Med Assoc","first-page":"1759","article-title":"Comparison of bacteriologic culture of blood and necropsy specimens for determining the cause of foal septicemia: 47 cases (1978\u20131987)","volume":"195","author":"Wilson","year":"1989"},{"key":"ref5","series-title":"Langenbecks Arch Surg","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1007\/s00423-010-0658-6","article-title":"Endotoxin elimination in sepsis: physiology and therapeutic application","volume":"395","author":"Buttenschoen","year":"2010"},{"key":"ref6","series-title":"Equine Vet J","first-page":"371","article-title":"Endotoxaemia: a review with implications for the horse","volume":"37","author":"Werners","year":"2005"},{"key":"ref7","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1111\/j.1939-1676.1998.tb00493.x","article-title":"Hemostatic and fibrinolytic indices in neonatal foals with presumed septicemia","volume":"12","author":"Barton","year":"1998"},{"key":"ref8","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1111\/j.1939-1676.1993.tb01022.x","article-title":"Plasma endotoxin concentrations in clinically normal and potentially septic equine neonates","volume":"7","author":"Breuhaus","year":"1993"},{"key":"ref9","series-title":"Clin Perinatol","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1016\/j.clp.2010.04.002","article-title":"Pathophysiology and treatment of septic shock in neonates","volume":"37","author":"Wynn","year":"2010"},{"key":"ref10","series-title":"Clin Microbiol Rev","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1128\/CMR.16.4.637-646.2003","article-title":"Role of Toll-like receptors in pathogen recognition","volume":"16","author":"Janssens","year":"2003"},{"key":"ref11","series-title":"Nat Rev Immunol","doi-asserted-by":"publisher","first-page":"512","DOI":"10.1038\/nri1396","article-title":"Therapeutics targeting the innate immune system","volume":"4","author":"Ulevitch","year":"2004"},{"key":"ref12","series-title":"N Engl J Med","doi-asserted-by":"publisher","first-page":"699","DOI":"10.1056\/NEJM200103083441001","article-title":"Efficacy and safety of recombinant human activated protein C for severe sepsis","volume":"344","author":"Bernard","year":"2001"},{"key":"ref13","series-title":"Crit Care","first-page":"R27","article-title":"Toxicity of polymyxins: a systematic review of the evidence from old and recent studies","volume":"10","author":"Falagas","year":"2006"},{"key":"ref14","series-title":"Mol Biosyst","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1039\/b500756a","article-title":"Polymyxin B: an ode to an old antidote for endotoxic shock","volume":"1","author":"Bhor","year":"2005"},{"key":"ref15","series-title":"Equine Vet J","first-page":"397","article-title":"Polymyxin B protects horses against induced endotoxaemia in vivo","volume":"36","author":"Barton","year":"2004"},{"key":"ref16","series-title":"Am J Vet Res","first-page":"921","article-title":"Effects of polymyxin B and Salmonella typhimurium antiserum on horses given endotoxin intravenously","volume":"55","author":"Durando","year":"1994"},{"key":"ref17","series-title":"Vet Hum Toxicol","first-page":"422","article-title":"Effects of polymyxin B on selected features of equine carbohydrate overload","volume":"31","author":"Raisbeck","year":"1989"},{"key":"ref18","series-title":"Am J Vet Res","first-page":"68","article-title":"Effect of a conjugate of polymyxin B-dextran 70 in horses with experimentally induced endotoxaemia","volume":"60","author":"MacKay","year":"1999"},{"key":"ref19","series-title":"Am J Vet Res","first-page":"71","article-title":"Evaluation of polymyxin B in an ex vivo model of endotoxemia in horses","volume":"62","author":"Parviainen","year":"2001"},{"key":"ref20","series-title":"Aust Vet J","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1111\/j.1751-0813.2005.tb12191.x","article-title":"Equine endotoxemia\u2014a state-of-the-art review of therapy","volume":"83","author":"Sykes","year":"2005"},{"key":"ref21","series-title":"Equine Vet J","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1111\/j.2042-3306.1990.tb04198.x","article-title":"Haemodynamic, pathological, haematological and behavioural changes during endotoxin infusion in equine neonates","volume":"22","author":"Lavoie","year":"1990"},{"key":"ref22","series-title":"Am J Vet Res","first-page":"1404","article-title":"Serum tumor necrosis factor alpha concentrations and clinical abnormalities in colostrum-fed and colostrum-deprived neonatal foals given endotoxin","volume":"54","author":"Allen","year":"1993"},{"key":"ref23","series-title":"Am J Vet Res","first-page":"1411","article-title":"Serum interleukin-6 concentrations in endotoxin-infused neonatal foals","volume":"54","author":"Robinson","year":"1993"},{"key":"ref24","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"1090","DOI":"10.1111\/j.1939-1676.2007.tb03069.x","article-title":"Assessment of a point-of-care lactate monitor in emergency admissions of adult horses to a referral hospital","volume":"21","author":"Tennent-Brown","year":"2007"},{"key":"ref25","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"617","DOI":"10.1111\/j.1939-1676.2010.0481.x","article-title":"Evaluation of a veterinary glucometer for use in horses","volume":"24","author":"Hackett","year":"2010"},{"key":"ref26","series-title":"Am J Vet Res","first-page":"147","article-title":"Evaluation of a technique for measurement of gamma glutamylpeptidase in equine urine","volume":"46","author":"Adams","year":"1985"},{"key":"ref27","series-title":"J Am Vet Med Assoc","first-page":"1903","article-title":"Endotoxemia and septicemia in horses: experimental and clinical correlates","volume":"200","author":"Moore","year":"1992"},{"key":"ref28","series-title":"J Am Vet Med Assoc","first-page":"1584","article-title":"Tumor necrosis factor activity in serum from neonatal foals with presumed septicemia","volume":"199","author":"Morris","year":"1991"},{"key":"ref29","series-title":"J Reprod Fertil Suppl","first-page":"537","article-title":"Tissue glycogen and glucose 6-phosphatase levels in fetal and newborn foals","author":"Fowden","year":"1991"},{"key":"ref30","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"1223","DOI":"10.1111\/j.1939-1676.2008.0174.x","article-title":"Blood glucose concentrations in critically ill neonatal foals","volume":"22","author":"Hollis","year":"2008"},{"key":"ref31","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1111\/j.1939-1676.2010.0636.x","article-title":"Insulin, glucagon, and leptin in critically ill foals","volume":"25","author":"Barsnick","year":"2011"},{"key":"ref32","series-title":"Vet Clin North Am Equine Pract","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/j.cveq.2010.12.011","article-title":"Endocrine dysregulation in critically ill foals","volume":"27","author":"Toribio","year":"2011"},{"key":"ref33","series-title":"Equine Vet J","first-page":"53","article-title":"Arterial lactate concentration, hospital survival, sepsis and SIRS in critically ill neonatal foals","volume":"37","author":"Corley","year":"2005"},{"key":"ref34","series-title":"J Vet Emerg Crit Care","doi-asserted-by":"publisher","first-page":"496","DOI":"10.1111\/j.1476-4431.2008.00349.x","article-title":"Association of hyperlactatemia with age, diagnosis and survival in equine neonates","volume":"18","author":"Henderson","year":"2008"},{"key":"ref35","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"598","DOI":"10.1111\/j.1939-1676.2009.0277.x","article-title":"Association of blood lactate concentration and outcome in foals","volume":"23","author":"Wotman","year":"2009"},{"key":"ref36","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"198","DOI":"10.1111\/j.1939-1676.2009.0419.x","article-title":"Sequential plasma lactate concentrations as prognostic indicators in adult equine emergencies","volume":"24","author":"Tennent-Brown","year":"2010"},{"key":"ref37","series-title":"Equine Vet J Suppl","first-page":"57","article-title":"Association of admission l-lactate concentration in hospitalised equine neonates with presenting complaint, periparturient events, clinical diagnosis and outcome: a prospective multientre study","author":"Borchers","year":"2012"},{"key":"ref38","series-title":"Blood","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1182\/blood.V47.4.539.539","article-title":"Granulocyte function in experimental human endotoxemia","volume":"47","author":"Territo","year":"1976"},{"key":"ref39","series-title":"Am J Vet Res","first-page":"533","article-title":"Tumor necrosis factor activity in the circulation of horses given endotoxin","volume":"52","author":"MacKay","year":"1991"},{"key":"ref40","series-title":"Am Rev Respir Dis","doi-asserted-by":"publisher","first-page":"1095","DOI":"10.1164\/ajrccm\/144.5.1095","article-title":"Prostacyclin and thromboxane A2 formation is increased in human sepsis syndrome. Effects of cycloocygenase inhibition","volume":"144","author":"Bernard","year":"1991"},{"key":"ref41","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"642","DOI":"10.2460\/ajvr.67.4.642","article-title":"Endotoxin-neutralizing activity of polymyxin B in blood after IV administration in horses","volume":"67","author":"Morresey","year":"2006"},{"key":"ref42","series-title":"Prostaglandins Leukot Med","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1016\/0262-1746(87)90069-2","article-title":"Effects of multiple low doses of flunixin meglumine on repeated endotoxin challenge in the horse","volume":"27","author":"Semrad","year":"1987"},{"key":"ref43","series-title":"Am J Vet Res","first-page":"1300","article-title":"Effects of pentoxifyllin infusion on response of horses to in vivo challenge exposure with endotoxin","volume":"58","author":"Barton","year":"1997"},{"key":"ref44","series-title":"Equine Vet J","doi-asserted-by":"publisher","first-page":"358","DOI":"10.2746\/042516408X293501","article-title":"Evaluation of dimethyl sulphoxide effects on initial response to endotoxin in the horse","volume":"40","author":"Kelmer","year":"2008"},{"key":"ref45","series-title":"Pediatrics","doi-asserted-by":"publisher","first-page":"1248","DOI":"10.1542\/peds.2009-3274","article-title":"Mechanisms and regulation of the gene expression response to sepsis","volume":"125","author":"Cornell","year":"2010"},{"key":"ref46","series-title":"Equine clinical neonatology","first-page":"561","article-title":"Hematology and clinical chemistry","author":"Harvey","year":"1990"}],"container-title":["Journal of the American Veterinary Medical Association"],"original-title":[],"link":[{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/javma\/243\/6\/javma.243.6.874.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/javma\/243\/6\/javma.243.6.874.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/javma\/243\/6\/javma.243.6.874.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T11:40:44Z","timestamp":1741347644000},"score":1,"resource":{"primary":{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/javma\/243\/6\/javma.243.6.874.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9,15]]},"references-count":46,"journal-issue":{"issue":"6"},"URL":"https:\/\/doi.org\/10.2460\/javma.243.6.874","relation":{},"ISSN":["0003-1488"],"issn-type":[{"value":"0003-1488","type":"print"}],"subject":[],"published":{"date-parts":[[2013,9,15]]}}}