{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T15:03:33Z","timestamp":1779894213418,"version":"3.53.1"},"reference-count":16,"publisher":"American Veterinary Medical Association (AVMA)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["javma"],"published-print":{"date-parts":[[2014,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To determine the feasibility of qualifying individuals or groups of Yellowstone National Park bison as free from brucellosis.<\/jats:p>\n<jats:p><jats:bold>Design<\/jats:bold>\u2014Cohort study.<\/jats:p>\n<jats:p><jats:bold>Sample<\/jats:bold>\u2014Serum, blood, and various samples from live bison and tissues taken at necropsy from 214 bison over 7 years.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014Blood was collected from bison every 30 to 45 days for serologic tests and microbiological culture of blood for <jats:italic>Brucella abortus<\/jats:italic>. Seropositive bison were euthanized until all remaining bison had 2 consecutive negative test results. Half the seronegative bison were randomly euthanized, and tissues were collected for bacteriologic culture. The remaining seronegative bison were bred, and blood was tested at least twice per year. Cow-calf pairs were sampled immediately after calving and 6 months after calving for evidence of <jats:italic>B abortus<\/jats:italic>.<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014Post-enrollment serial testing for <jats:italic>B abortus<\/jats:italic> antibodies revealed no bison that seroconverted after 205 days (first cohort) and 180 days (second cohort). During initial serial testing, 85% of bison seroconverted within 120 days after removal from the infected population. <jats:italic>Brucella abortus<\/jats:italic> was not cultured from any euthanized seronegative bison (0\/88). After parturition, no cows or calves had a positive test result for <jats:italic>B abortus<\/jats:italic> antibodies, nor was <jats:italic>B abortus<\/jats:italic> cultured from any samples.<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Results suggested it is feasible to qualify brucellosis-free bison from an infected herd following quarantine procedures as published in the USDA APHIS brucellosis eradication uniform methods and rules. Latent infection was not detected in this sample of bison when applying the USDA APHIS quarantine protocol.<\/jats:p>","DOI":"10.2460\/javma.244.5.588","type":"journal-article","created":{"date-parts":[[2014,2,18]],"date-time":"2014-02-18T15:48:26Z","timestamp":1392738506000},"page":"588-591","source":"Crossref","is-referenced-by-count":8,"title":["Feasibility of quarantine procedures for bison (Bison bison) calves from Yellowstone National Park for conservation of brucellosis-free bison"],"prefix":"10.2460","volume":"244","author":[{"given":"P.","family":"Ryan Clarke","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca K.","family":"Frey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack C.","family":"Rhyan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matt P.","family":"McCollum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauline","family":"Nol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith","family":"Aune","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"Final environmental impact statement for the interagency bison management plan for the State of Montana and Yellowstone National Park","year":"2000"},{"key":"ref2","series-title":"Veterinary services publication 91\u201345\u2013013","article-title":"Brucellosis eradication: uniform methods and rules","year":"2003"},{"key":"ref3","series-title":"National Veterinary Services Laboratories protocol SOP-SERO-1027.04","first-page":"1","article-title":"Card test for detection of antibodies to Brucella abortus","year":"2006"},{"key":"ref4","series-title":"National Veterinary Services Laboratories protocol SEROPRO1051.03","first-page":"1","article-title":"fluorescence polarization assay (FPA) using tubes","year":"2006"},{"key":"ref5","series-title":"Complement fixation test for detection of antibodies to Brucella abortus and Brucella suis. National Veterinary Services Laboratories protocol SOP-SERO-1022.09","first-page":"1","year":"2011"},{"key":"ref6","series-title":"National Veterinary Services Laboratories protocol SOP-SERO-1026.06","first-page":"1","article-title":"Tube agglutination test for detection of antibodies to Brucella abortus","year":"2006"},{"key":"ref7","series-title":"Plate agglutination test for detection of antibodies to Brucella abortus. National Veterinary Services Laboratories protocol SOP-SERO-1023.04","first-page":"1","year":"2006"},{"key":"ref8","series-title":"National Veterinary Services Laboratories protocol SOP-SERO-1024.04","first-page":"1","article-title":"Buffered acidified plate antigen test for detection of antibodies to Brucella abortus","year":"2006"},{"key":"ref9","series-title":"National Veterinary Services Laboratories protocol SOP-SERO-1025.07","first-page":"1","article-title":"Rivanol precipitation-plate agglutination test for detection of antibodies to Brucella abortus\/suis","year":"2006"},{"key":"ref10","series-title":"Techniques for the brucellosis laboratory","first-page":"34","author":"Alton","year":"1988"},{"key":"ref11","series-title":"J Vet Diagn Invest","doi-asserted-by":"publisher","first-page":"374","DOI":"10.1177\/104063870301500413","article-title":"Evaluation of the Brucella abortus species-specific polymerase chain reaction assay, an improved version of the Brucella AMOS polymerase chain reaction assay for cattle","volume":"15","author":"Bricker","year":"2003"},{"key":"ref12","series-title":"Methods in molecular biology: PCR detection of microbial pathogens: methods and protocols","first-page":"97","article-title":"Identification and differentiation of Brucella abortus field and vaccine strains by BASS-PCR","volume":"216","author":"Ewalt","year":"2003"},{"key":"ref13","series-title":"Southwest Nat","doi-asserted-by":"publisher","first-page":"23","DOI":"10.2307\/3668811","article-title":"Bison (Bovidae) productivity on the Wichita Mountains Wildlife Refuge. Oklahoma","volume":"13","author":"Halloran","year":"1968"},{"key":"ref14","series-title":"Bison production research","first-page":"1","author":"Rutley","year":"2000"},{"key":"ref15","series-title":"J Wildl Manage","doi-asserted-by":"publisher","first-page":"1132","DOI":"10.2307\/3802831","article-title":"Brucellosis in Yellowstone National Park bison: quantitative serology and infection","volume":"63","author":"Roffe","year":"1999"},{"key":"ref16","series-title":"J Appl Ecol","doi-asserted-by":"publisher","first-page":"1324","DOI":"10.1111\/j.1365-2664.2011.02058.x","article-title":"Estimating probabilities of active brucellosis infection in Yellowstone bison through quantitative serology and tissue culture","volume":"48","author":"Treanor","year":"2011"}],"container-title":["Journal of the American Veterinary Medical Association"],"original-title":[],"link":[{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/javma\/244\/5\/javma.244.5.588.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/javma\/244\/5\/javma.244.5.588.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/javma\/244\/5\/javma.244.5.588.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T11:43:18Z","timestamp":1741347798000},"score":1,"resource":{"primary":{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/javma\/244\/5\/javma.244.5.588.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,3,1]]},"references-count":16,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.2460\/javma.244.5.588","relation":{},"ISSN":["0003-1488"],"issn-type":[{"value":"0003-1488","type":"print"}],"subject":[],"published":{"date-parts":[[2014,3,1]]}}}