{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T16:22:43Z","timestamp":1770222163924,"version":"3.49.0"},"reference-count":25,"publisher":"American Veterinary Medical Association (AVMA)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["javma"],"published-print":{"date-parts":[[2015,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Case Description<\/jats:bold>\u2014A 3-year-old 10-kg (22-lb) neutered male Cavalier King Charles Spaniel was referred because of an episode of acute vomiting and diarrhea.<\/jats:p>\n<jats:p><jats:bold>Clinical Findings<\/jats:bold>\u2014On physical examination, mild splenomegaly and prominent submandibular and popliteal lymph nodes were detected. Complete blood cell count revealed a high WBC count, characterized by a moderate lymphocytosis with 62% unclassified cells and severe thrombocytopenia with macroplatelets. On cytologic evaluation, the unclassified cells were described as large, neoplastic lymphoid cells containing a large nucleus with lacy chromatin and a large amount of blue vacuolated cytoplasm containing sparse, very fine azurophilic granules. A diagnosis of acute large granular lymphocytic leukemia of splenic origin was made.<\/jats:p>\n<jats:p><jats:bold>Treatment and Outcome<\/jats:bold>\u2014Following induction chemotherapy, the affected dog underwent allogeneic hematopoietic cell transplantation with dog leukocyte antigen\u2013matched CD34+ cells harvested from a sibling of the same litter. Chimerism analysis revealed full donor engraftment within 2 weeks after transplantation that remained stable for at least 2 years, with the dog remaining apparently healthy at home.<\/jats:p>\n<jats:p><jats:bold>Clinical Relevance<\/jats:bold>\u2014Acute leukemias in dogs are rapidly fatal diseases. If an appropriate donor can be located, allogeneic hematopoietic cell transplantation may offer a feasible treatment, although peripheral blood CD34+ cell harvesting requires the availability of cell separator machines and management of graft-versus-host disease with immunosuppressive agents.<\/jats:p>","DOI":"10.2460\/javma.246.9.994","type":"journal-article","created":{"date-parts":[[2015,4,15]],"date-time":"2015-04-15T14:50:38Z","timestamp":1429109438000},"page":"994-997","source":"Crossref","is-referenced-by-count":13,"title":["Allogeneic hematopoietic cell transplantation in a dog with acute large granular lymphocytic leukemia"],"prefix":"10.2460","volume":"246","author":[{"given":"Steven E.","family":"Suter","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew J","family":"Hamilton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edmund 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