{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T05:07:52Z","timestamp":1780636072910,"version":"3.54.1"},"reference-count":46,"publisher":"American Veterinary Medical Association (AVMA)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["javma"],"published-print":{"date-parts":[[2016,9,15]]},"abstract":"Abstract<\/jats:title>\nOBJECTIVE<\/jats:bold> To develop and evaluate a protocol for control of Giardia duodenalis<\/jats:italic> in naturally infected group-housed dogs at a veterinary medical college.<\/jats:p>\nDESIGN<\/jats:bold> Prospective evaluation study.<\/jats:p>\nANIMALS<\/jats:bold> 34 dogs.<\/jats:p>\nPROCEDURES<\/jats:bold> All dogs were tested for evidence of G duodenalis<\/jats:italic> infection. Dogs were treated with fenbendazole on study days 1 through 10. On day 5, dogs were bathed and moved into clean, disinfected kennels in a different room to allow for disinfection and drying of their assigned kennels at 26.7\u00b0C (80\u00b0F) for 24 hours on day 6. After treatment, dogs were returned to their original housing; fecal samples were collected weekly from days 8 through 41 and then every 3 weeks until day 209. Samples were fixed in formalin and examined by direct immunofluorescence assay. Additionally, 1 pretreatment sample underwent PCR assay and DNA sequencing to determine the assemblage (genotype) of the organism. Normal handling routines for the dogs and their use in teaching activities were not changed.<\/jats:p>\nRESULTS<\/jats:bold> Initially, all dogs in the colony shed G duodenalis<\/jats:italic> cysts. During and immediately after treatment (days 8 and 13), no cysts were detected in any dogs. On day 20, 1 cyst was observed in the fecal sample from 1 dog; results for all subsequent fecal analyses were negative. The G duodenalis<\/jats:italic> cysts collected from the pretreatment sample had an assemblage C genotype.<\/jats:p>\nCONCLUSIONS AND CLINICAL RELEVANCE<\/jats:bold> The integrated protocol was successful in controlling G duodenalis<\/jats:italic> infection in this dog colony, despite exposure of dogs to a variety of environments and frequent handling by multiple individuals. Sequence analysis identified an assemblage typically found in dogs but not in people, indicating that zoonotic transmission would be unlikely.<\/jats:p>","DOI":"10.2460\/javma.249.6.644","type":"journal-article","created":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T15:20:15Z","timestamp":1472743215000},"page":"644-649","source":"Crossref","is-referenced-by-count":10,"title":["Development and evaluation of a protocol for control of Giardia duodenalis in a colony of group-housed dogs at a veterinary medical college"],"prefix":"10.2460","volume":"249","author":[{"given":"Meriam N.","family":"Saleh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra D.","family":"Gilley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meghan K.","family":"Byrnes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne 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