{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,17]],"date-time":"2025-05-17T04:10:50Z","timestamp":1747455050664,"version":"3.40.5"},"reference-count":33,"publisher":"American Veterinary Medical Association (AVMA)","issue":"S1","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["javma"],"published-print":{"date-parts":[[2025,6,1]]},"abstract":"Abstract<\/jats:title>\n\nObjective<\/jats:title>\nPituitary pars intermedia dysfunction is diagnosed using immunoreactive ACTH concentrations. Glass reportedly binds ACTH, but the clinical relevance of using glass blood tubes for measuring equine ACTH concentrations is controversial. This study investigates whether the collection of whole blood into glass EDTA tubes and storage for up to 6 hours changes ACTH concentrations compared to plastic EDTA tubes.<\/jats:p>\n<\/jats:sec>\n\nMethods<\/jats:title>\nThis was a cohort study. Whole blood was collected into both glass and plastic EDTA tubes from 9 horses > 12 years of age, including 4 with pituitary pars intermedia dysfunction and 5 controls. Samples were centrifuged immediately or stored in the collection tube at 4\u2009\u00b0C for 2, 4, or 6 hours before centrifugation. Plasma was separated into cryovials, and immunoreactive ACTH was immediately measured on a chemiluminescent assay. Data were analyzed using a 2-way repeated-measures ANOVA with P<\/jats:italic> < .05 considered significant.<\/jats:p>\n<\/jats:sec>\n\nResults<\/jats:title>\nNeither tube type nor storage time had a significant effect on plasma immunoreactive ACTH concentration.<\/jats:p>\n<\/jats:sec>\n\nConclusions<\/jats:title>\nWhen stored at 4\u2009\u00b0C as whole blood on glass for up to 6 hours, equine immunoreactive ACTH concentrations do not significantly differ from collection and storage on plastic.<\/jats:p>\n<\/jats:sec>\n\nClinical Relevance<\/jats:title>\nBoth glass and plastic EDTA tubes can be used for collection and same-day analysis of immunoreactive ACTH concentrations.<\/jats:p>\n<\/jats:sec>","DOI":"10.2460\/javma.25.02.0122","type":"journal-article","created":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T15:12:32Z","timestamp":1743088352000},"page":"S71-S75","source":"Crossref","is-referenced-by-count":0,"title":["Collecting equine whole blood in glass tubes does not decrease adrenocorticotropic hormone concentration compared to plastic tubes"],"prefix":"10.2460","volume":"263","author":[{"given":"Emma D.","family":"Stapley","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Purdue University, West Lafayette, IN"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2820-8431","authenticated-orcid":true,"given":"Fran\u00e7ois-Ren\u00e9","family":"Bertin","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Purdue University, West Lafayette, IN"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"key":"B1","series-title":"Vet J","first-page":"22","article-title":"Epidemiology of pituitary pars intermedia dysfunction: a systematic literature review of clinical presentation, disease prevalence and risk factors","volume":"235","author":"Ireland JL","year":"2018","unstructured":"Ireland JL, McGowan CM. Epidemiology of pituitary pars intermedia dysfunction: a systematic literature review of clinical presentation, disease prevalence and risk factors. Vet J. 2018;235:22\u201333. doi:10.1016\/j.tvjl.2018.03.002"},{"issue":"2","key":"B2","series-title":"J Neuroendocrinol","first-page":"73","article-title":"Nitration and increased \u03b1-synuclein expression associated with dopaminergic neurodegeneration in equine pituitary pars intermedia dysfunction","volume":"17","author":"McFarlane D","year":"2005","unstructured":"McFarlane D, Dybdal N, Donaldson MT, Miller L, Cribb AE. Nitration and increased \u03b1-synuclein expression associated with dopaminergic neurodegeneration in equine pituitary pars intermedia dysfunction. 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