{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T18:23:39Z","timestamp":1787336619007,"version":"build-2736575974"},"reference-count":23,"publisher":"American Veterinary Medical Association (AVMA)","issue":"9","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["javma"],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To compare canine parvovirus (CPV) antibody titers in sera of community blood-donor dogs using the hemagglutination inhibition (HI) assays from 2 state veterinary diagnostic laboratories (VDLs) and a dot-blot ELISA assay to determine intermethod agreement.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Stored sera (\u201380-\u00b0C freezer) from 100 healthy community-based blood-donor dogs were selected. Dogs represented various breeds and ages. Available vaccination history was retrospectively assessed. Sera were thawed and delivered to the VDLs, with HI and the dot-blot ELISA assay performed the following day.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Of 100 samples, 96 dot-blot ELISA results were within a 2-fold dilution of the corresponding HI titer from the Colorado State University VDL, and 92 were within a 2-fold dilution of the HI titer from the New York State VDL. The dot-blot ELISA assay demonstrated high sensitivity (96% and 97%) and strong agreement (Spearman \u03c1 = 0.72 and 0.92). Of 43 dogs with vaccination history, 93% had protective titers. The 3 lowest titers occurred in dogs vaccinated within 1 year.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>CPV antibody titers in community blood-donor dogs showed strong agreement between HI assays and the dot-blot ELISA assay. A high concordance and strong correlation support the dot-blot ELISA assay as a reliable method for estimating CPV antibody titers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical Relevance<\/jats:title>\n                    <jats:p>Accurate, rapid assessment of CPV titers is important for selecting plasma from community blood-donor dogs intended for passive immunotherapy in critically ill parvoviral patients. Demonstration of strong agreement between the dot-blot ELISA and reference HI assays supports the use of this assay for in-house donor screening and timely plasma selection.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.2460\/javma.25.12.0808","type":"journal-article","created":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T21:06:10Z","timestamp":1775682370000},"page":"1-7","source":"Crossref","is-referenced-by-count":0,"title":["Dot-blot ELISA assay demonstrates strong agreement with hemagglutination inhibition for the detection of canine parvovirus antibodies in sera of healthy, vaccinated blood-donor dogs"],"prefix":"10.2460","volume":"264","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6136-1991","authenticated-orcid":true,"given":"Charles T.","family":"Talbot","sequence":"first","affiliation":[{"name":"Center for Companion Animal Studies, Translational Medicine Institute, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Brewer","sequence":"additional","affiliation":[{"name":"Center for Companion Animal Studies, Translational Medicine Institute, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"McKenna","family":"Willis","sequence":"additional","affiliation":[{"name":"Center for Companion Animal Studies, Translational Medicine Institute, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kris","family":"Kofron","sequence":"additional","affiliation":[{"name":"Center for Companion Animal Studies, Translational Medicine Institute, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah B.","family":"Shropshire","sequence":"additional","affiliation":[{"name":"Center for Companion Animal Studies, Translational Medicine Institute, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly S.","family":"Santangelo","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology & Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0327-8537","authenticated-orcid":true,"given":"Kristin M.","family":"Zersen","sequence":"additional","affiliation":[{"name":"Center for Companion Animal Studies, Translational Medicine Institute, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Lappin","sequence":"additional","affiliation":[{"name":"Center for Companion Animal Studies, Translational Medicine Institute, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"issue":"6","key":"ref1","series-title":"Vet Clin North Am Small Anim Pract","first-page":"1307","article-title":"Update on canine parvoviral enteritis","volume":"50","author":"Mazzaferro EM","year":"2020","unstructured":"Mazzaferro EM. 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Vet J. 2023;298-299:106017. doi:10.1016\/j.tvjl.2023.106017"}],"container-title":["Journal of the American Veterinary Medical Association"],"original-title":[],"link":[{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/javma\/264\/9\/javma.25.12.0808.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/javma\/264\/9\/javma.25.12.0808.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/javma\/264\/9\/javma.25.12.0808.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T17:24:17Z","timestamp":1787333057000},"score":1,"resource":{"primary":{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/javma\/264\/9\/javma.25.12.0808.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,1]]},"references-count":23,"journal-issue":{"issue":"9"},"URL":"https:\/\/doi.org\/10.2460\/javma.25.12.0808","relation":{},"ISSN":["0003-1488"],"issn-type":[{"value":"0003-1488","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9,1]]}}}