{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T06:19:39Z","timestamp":1783577979508,"version":"3.55.0"},"reference-count":61,"publisher":"American Veterinary Medical Association (AVMA)","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["javma"],"published-print":{"date-parts":[[2020,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:sec id=\"s001\">\n<jats:title>CASE DESCRIPTION<\/jats:title>\n<jats:p>A 12-year-old neutered male domestic shorthair cat with chronic anterior uveitis and secondary glaucoma of the right eye was examined for persistent blepharospasm 2 weeks after corneal debridement and grid keratotomy for nonhealing superficial ulcerative keratitis.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"s002\">\n<jats:title>CLINICAL FINDINGS<\/jats:title>\n<jats:p>Examination of the right eye revealed a central superficial corneal ulcer associated with corneal epithelial and subepithelial infiltrates and mild aqueous flare. Structures consistent with amoeboid cysts and trophozoites were detected in the cornea by in vivo confocal microscopy. Suppurative keratitis was identified cytologically. An <jats:italic>Acanthamoeba<\/jats:italic> spp was isolated through culture and identified by a PCR assay of corneal specimens.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"s003\">\n<jats:title>TREATMENT AND OUTCOME<\/jats:title>\n<jats:p>Symptomatic and antiamoebic (polyhexamethylene biguanide 0.02% ophthalmic solution) treatments were instituted. Over the following 6 weeks, the cat lost vision in the affected eye and lesions progressed to nonulcerative stromal keratitis associated with a dense paracentral corneal stroma ring infiltrate and anterior lens luxation. The globe was enucleated, and lymphoplasmacytic sclerokeratitis, anterior uveitis, and retinal detachment were noted. <jats:italic>Acanthamoeba<\/jats:italic> organisms were detected within the corneal stroma and anterior sclera with histologic and immunohistochemical stains. The amoebae were classified to the <jats:italic>Acanthamoeba<\/jats:italic> T4 genotype by DNA sequencing. The cat had no medical problems attributed to <jats:italic>Acanthamoeba<\/jats:italic> infection over 36 months after enucleation, until the cat was lost to follow-up.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"s004\">\n<jats:title>CLINICAL RELEVANCE<\/jats:title>\n<jats:p>Naturally acquired <jats:italic>Acanthamoeba<\/jats:italic> sclerokeratitis is described in a cat for the first time. <jats:italic>Acanthamoeba<\/jats:italic> infection should be considered for cats with superficial corneal disease refractory to appropriate treatments and especially occurring after ocular trauma, including keratotomy.<\/jats:p>\n<\/jats:sec>","DOI":"10.2460\/javma.257.12.1280","type":"journal-article","created":{"date-parts":[[2020,12,3]],"date-time":"2020-12-03T15:33:08Z","timestamp":1607009588000},"page":"1280-1287","source":"Crossref","is-referenced-by-count":7,"title":["Acanthamoeba sclerokeratitis in a cat"],"prefix":"10.2460","volume":"257","author":[{"given":"Eric 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