{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T03:57:38Z","timestamp":1793246258219,"version":"4.5.0"},"reference-count":37,"publisher":"American Veterinary Medical Association (AVMA)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["javma"],"published-print":{"date-parts":[[2021,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:sec id=\"s001\">\n<jats:title>OBJECTIVE<\/jats:title>\n<jats:p>To evaluate the utility of an automated insulated isothermal PCR (iiPCR) system for rapid and reliable on-site detection of African swine fever virus (ASFV) in swine biological samples.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"s002\">\n<jats:title>SAMPLE<\/jats:title>\n<jats:p>Lymph node, tissue homogenate, whole blood, serum, spleen, and tonsil samples collected from swine in North and South Vietnam.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"s003\">\n<jats:title>PROCEDURES<\/jats:title>\n<jats:p>Analytic sensitivity of the iiPCR system was determined by serial dilution and analysis of 2 samples (swine tissue homogenate and blood) predetermined to be positive for ASFV. Analytic specificity was assessed by analysis of 2 samples predetermined to be negative for ASFV and positive or negative for other swine pathogens (classical swine fever virus, porcine reproductive and respiratory syndrome virus, foot-and-mouth disease virus, and porcine circovirus type 2). Diagnostic performance of the iiPCR system for detection of ASFV was determined by analysis of the various tissue sample types. For all tests, a real-time PCR assay was used as the reference method.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"s004\">\n<jats:title>RESULTS<\/jats:title>\n<jats:p>The iiPCR system was able to detect ASFV in swine blood or tissue homogenate at dilutions up to 10<jats:sup>6<\/jats:sup>, whereas the real-time PCR assay was able to detect dilutions of up to 10<jats:sup>5<\/jats:sup> or 10<jats:sup>6<\/jats:sup>. The iiPCR system had high analytic specificity for detection of ASFV versus other swine pathogens. Between 97% and 100% agreement was found between results of the iiPCR system for the various tissue samples and results of real-time PCR assay.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"s005\">\n<jats:title>CONCLUSIONS AND CLINICAL RELEVANCE<\/jats:title>\n<jats:p>The evaluated iiPCR system was found to be a rapid, reliable, and sample-flexible method for ASFV detection and may be useful for disease surveil-lance and quarantine in national strategies for early ASF control.<\/jats:p>\n<\/jats:sec>","DOI":"10.2460\/javma.259.6.662","type":"journal-article","created":{"date-parts":[[2021,8,27]],"date-time":"2021-08-27T15:37:56Z","timestamp":1630078676000},"page":"662-668","source":"Crossref","is-referenced-by-count":5,"title":["Evaluation of an automated insulated isothermal polymerase chain reaction system for rapid and reliable, on-site detection of African swine fever virus"],"prefix":"10.2460","volume":"259","author":[{"given":"Huyen Nga Thi","family":"Tran","sequence":"first","affiliation":[{"name":"From the VNU University of Science, Vietnam National University-Hanoi, Hanoi, 11400 Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nhi-Cong Thi","family":"Le","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Graduate University of Science and Technology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bang Phuong","family":"Pham","sequence":"additional","affiliation":[{"name":"Vietnam Academy of Science and Technology, Hanoi, 11300 Vietnam; Thai Nguyen University of Agriculture and Forestry, Thai Nguyen, 24100 Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Van Quynh","family":"Luu","sequence":"additional","affiliation":[{"name":"Kim Nguu Instrument and Chemical Import-Export JSC, Hanoi, 11600 Vietnam."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viet-Linh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Graduate University of Science and Technology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"key":"R1","series-title":"Viruses","doi-asserted-by":"publisher","first-page":"103","DOI":"10.3390\/v9050103","article-title":"African swine fever virus: a review","volume":"9","author":"Galindo I","year":"2017","unstructured":"Galindo I, Alonso C. 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