{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:51:32Z","timestamp":1792173092225,"version":"4.4.0"},"reference-count":28,"publisher":"American Veterinary Medical Association (AVMA)","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["javma"],"published-print":{"date-parts":[[2026,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To compare clinical, pulmonary, and immunologic responses to bovine respiratory syncytial virus (BRSV) challenge in colostrum-fed, BRSV-seropositive calves following administration of 2 commercially available intranasal vaccines.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>34 male Holstein calves (9 to 20 days old) were randomly assigned to receive vaccine A (Nasalgen; n = 17) or vaccine B (Inforce 3; 17). Nine weeks after vaccination, calves were challenged with virulent BRSV. Clinical observations were recorded daily. Arterial partial pressure of oxygen was measured on day 6 after challenge, and percentage pneumonic lung involvement was assessed at necropsy on day 8. Antibody responses and viral shedding were assessed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Clinical signs were infrequent and mild in both groups, and nasal shedding of BRSV was low. Median lung lesion scores were lower in calves receiving vaccine B than in calves receiving vaccine A (2.3% vs 4.6%). Arterial oxygen concentrations were numerically higher in vaccine B calves (median, 91.0 mm Hg) than in vaccine A calves (median, 82.8 mm Hg), although this difference was not significant. A greater proportion of vaccine B calves had Pao\n                      <jats:sub>2<\/jats:sub>\n                      values within the normal range (&gt; 87.1 mm Hg) compared to vaccine A calves (12 of 17 [71%] vs 7 of 17 [41%]). Both vaccines induced anamnestic mucosal and systemic antibody responses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Both vaccines were associated with minimal clinical disease following BRSV challenge.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical Relevance<\/jats:title>\n                    <jats:p>Results suggest a modest advantage of vaccine B with respect to pulmonary involvement and maintenance of arterial oxygenation under these experimental conditions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.2460\/javma.26.04.0247","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T19:19:00Z","timestamp":1786562340000},"page":"1505-1511","source":"Crossref","is-referenced-by-count":0,"title":["Comparative clinical, immunological, and pulmonary responses to virulent bovine respiratory syncytial virus infection in colostrum-fed, intranasally vaccinated calves"],"prefix":"10.2460","volume":"264","author":[{"given":"John","family":"Ellis","sequence":"first","affiliation":[{"name":"Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheryl","family":"Gow","sequence":"additional","affiliation":[{"name":"Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Sniatynski","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacey","family":"Lacoste","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"Mononegaviruses of Veterinary Importance","first-page":"170","article-title":"Bovine respiratory syncytial virus","volume":"1","author":"Ellis JA","year":"2013","unstructured":"Ellis JA. Bovine respiratory syncytial virus. In: Munir M, ed. Mononegaviruses of Veterinary Importance. Vol 1. CAB International; 2013:170\u2013184."},{"issue":"8","key":"ref2","series-title":"Arch Virol","first-page":"2493","article-title":"Taxonomy of the order Mononegavirales: update 2017","volume":"162","author":"Amarasinghe GK","year":"2017","unstructured":"Amarasinghe GK, B\u00e0o Y, Basler CF,  Taxonomy of the order Mononegavirales: update 2017. Arch Virol. 2017;162(8):2493-2504. doi:10.1007\/s00705-017-3311-7"},{"key":"ref3","series-title":"Vet Microbiol","first-page":"59","article-title":"How efficacious are vaccines against bovine respiratory syncytial virus in cattle?","volume":"206","author":"Ellis JA","year":"2017","unstructured":"Ellis JA. How efficacious are vaccines against bovine respiratory syncytial virus in cattle? Vet Microbiol. 2017;206:59-68. doi:10.1016\/j.vetmic.2016.11.030"},{"issue":"22","key":"ref4","series-title":"Vaccine","first-page":"3784","article-title":"Immunogenicity of a modified-live virus vaccine against bovine viral diarrhea virus types 1 and 2, infectious bovine rhinotracheitis virus, bovine parainfluenza-3 virus, and bovine respiratory syncytial virus when administered intranasally in young calves","volume":"28","author":"Xue W","year":"2010","unstructured":"Xue W, Ellis J, Mattick D, Smith L, Brady R, Trigo E. Immunogenicity of a modified-live virus vaccine against bovine viral diarrhea virus types 1 and 2, infectious bovine rhinotracheitis virus, bovine parainfluenza-3 virus, and bovine respiratory syncytial virus when administered intranasally in young calves. Vaccine. 2010;28(22):3784-3792. doi:10.1016\/j.vaccine.2010.03.043"},{"issue":"11","key":"ref5","series-title":"J Am Vet Med Assoc","first-page":"1602","article-title":"Duration of immunity to experimental infection with bovine respiratory syncytial virus following intranasal vaccination of young passively immune calves","volume":"243","author":"Ellis JA","year":"2013","unstructured":"Ellis JA, Gow SP, Mahan S, Leyh R. Duration of immunity to experimental infection with bovine respiratory syncytial virus following intranasal vaccination of young passively immune calves. J Am Vet Med Assoc. 2013;243(11):1602-1608. doi:10.2460\/javma.243.11.1602"},{"issue":"11","key":"ref6","series-title":"Vet Rec","first-page":"e83","article-title":"NASYM, a live intranasal vaccine, protects young calves from bovine respiratory syncytial virus in the presence of maternal antibodies","volume":"188","author":"Marzo E","year":"2021","unstructured":"Marzo E, Montbrau C, Moreno MC,  NASYM, a live intranasal vaccine, protects young calves from bovine respiratory syncytial virus in the presence of maternal antibodies. Vet Rec. 2021;188(11):e83. doi:10.1002\/vetr.83"},{"issue":"6","key":"ref7","series-title":"Pathogens","first-page":"517","article-title":"The detection of vaccine virus and protection of a modified live, intranasal, trivalent vaccine in neonatal, colostrum-fed calves with an experimental bovine respiratory syncytial virus challenge","volume":"13","author":"Perkins-Oines S","year":"2024","unstructured":"Perkins-Oines S, Senevirathne ND, Krafsur GM,  The detection of vaccine virus and protection of a modified live, intranasal, trivalent vaccine in neonatal, colostrum-fed calves with an experimental bovine respiratory syncytial virus challenge. Pathogens. 2024;13(6):517. doi:10.3390\/pathogens13060517"},{"key":"ref8","series-title":"Vet J","first-page":"105532","article-title":"Effectiveness of two intranasal vaccines for the control of bovine respiratory disease in newborn beef calves: a randomized non-inferiority multicentre field trial","volume":"263","author":"Masset N","year":"2020","unstructured":"Masset N, Meurens F, Marie M,  Effectiveness of two intranasal vaccines for the control of bovine respiratory disease in newborn beef calves: a randomized non-inferiority multicentre field trial. Vet J. 2020;263:105532. doi:10.1016\/j.tvjl.2020.105532"},{"issue":"3","key":"ref9","series-title":"Can J Vet Res","first-page":"218","article-title":"Genealogy of an in-vivo passaged isolate of western Canadian bovine respiratory syncytial virus","volume":"86","author":"Ellis J","year":"2022","unstructured":"Ellis J, Marx J, Perumbakkam S,  Genealogy of an in-vivo passaged isolate of western Canadian bovine respiratory syncytial virus. Can J Vet Res. 2022;86(3):218-228."},{"issue":"9","key":"ref10","series-title":"J Am Vet Med Assoc","first-page":"991","article-title":"Response to experimentally induced infection with bovine respiratory syncytial virus following intranasal vaccination of seropositive and seronegative calves","volume":"236","author":"Ellis JA","year":"2010","unstructured":"Ellis JA, Gow SP, Goji N. Response to experimentally induced infection with bovine respiratory syncytial virus following intranasal vaccination of seropositive and seronegative calves. J Am Vet Med Assoc. 2010;236(9):991-999. doi:10.2460\/javma.236.9.991"},{"issue":"3","key":"ref11","series-title":"J Appl Physiol","first-page":"400","article-title":"Cardiac catheterization of unanesthetized large domestic animals","volume":"33","author":"Will JA","year":"1972","unstructured":"Will JA, Bisgard GE. Cardiac catheterization of unanesthetized large domestic animals. J Appl Physiol. 1972;33(3):400-401. doi:10.1152\/jappl.1972.33.3.400"},{"key":"ref12","series-title":"Iowa State Univ Vet","first-page":"12","article-title":"Clinical pathology review: bovine blood gas analysis","volume":"43","author":"Hagemoser, WA","year":"1981","unstructured":"Hagemoser, WA, Lofstedt J. Clinical pathology review: bovine blood gas analysis. Iowa State Univ Vet. 1981;43:12-13."},{"issue":"1","key":"ref13","series-title":"Can J Vet Res","first-page":"28","article-title":"Functional analysis of antibody responses of feedlot cattle to bovine respiratory syncytial virus following vaccination with mixed vaccines","volume":"61","author":"West K","year":"1997","unstructured":"West K, Ellis J. Functional analysis of antibody responses of feedlot cattle to bovine respiratory syncytial virus following vaccination with mixed vaccines. Can J Vet Res. 1997;61(1):28-33."},{"issue":"3","key":"ref14","series-title":"Can J Vet Res","first-page":"163","article-title":"Infection of calves with in-vivo passaged bovine parainfluenza-3 virus, alone or in combination with bovine respiratory syncytial virus and bovine coronavirus","volume":"84","author":"Ellis J","year":"2020","unstructured":"Ellis J, Erickson N, Gow S, West K, Lacoste S, Godson D. Infection of calves with in-vivo passaged bovine parainfluenza-3 virus, alone or in combination with bovine respiratory syncytial virus and bovine coronavirus. Can J Vet Res. 2020;84(3):163-171."},{"issue":"2","key":"ref15","series-title":"J Virol Methods","first-page":"195","article-title":"Evaluation of a single-tube fluorogenic RT-PCR assay for detection of bovine respiratory syncytial virus in clinical samples","volume":"123","author":"Hakhverdyan M","year":"2005","unstructured":"Hakhverdyan M, H\u00e4gglund S, Larsen LE, Bel\u00e1k S. Evaluation of a single-tube fluorogenic RT-PCR assay for detection of bovine respiratory syncytial virus in clinical samples. J Virol Methods. 2005;123(2):195-202. doi:10.1016\/j.jviromet.2004.09.016"},{"issue":"2","key":"ref16","series-title":"J Am Vet Med Assoc","first-page":"267","article-title":"The perilous P value","volume":"263","author":"White CN","year":"2024","unstructured":"White CN. The perilous P value. J Am Vet Med Assoc. 2024;263(2):267-270. doi:10.2460\/javma.24.10.0645"},{"key":"ref17","series-title":"MSD Veterinary Manual","article-title":"Normal rectal temperature ranges","author":"MSD Veterinary Manual","unstructured":"MSD Veterinary Manual. Normal rectal temperature ranges. MSD Veterinary Manual. Accessed June 19, 2026. https:\/\/www.msdvetmanual.com\/reference-values-and-conversion-tables\/reference-guides\/normal-rectal-temperature-ranges"},{"issue":"10","key":"ref18","series-title":"Animals (Basel)","first-page":"754","article-title":"The 3Rs and humane experimental technique: implementing change","volume":"9","author":"Hubrecht RC","year":"2019","unstructured":"Hubrecht RC, Carter E. The 3Rs and humane experimental technique: implementing change. Animals (Basel). 2019;9(10):754. doi:10.3390\/ani9100754"},{"issue":"2","key":"ref19","series-title":"J Am Vet Med Assoc","first-page":"233","article-title":"Response of calves to challenge exposure with virulent bovine respiratory syncytial virus following intranasal administration of vaccines formulated for parenteral administration","volume":"230","author":"Ellis J","year":"2007","unstructured":"Ellis J, Gow S, West K,  Response of calves to challenge exposure with virulent bovine respiratory syncytial virus following intranasal administration of vaccines formulated for parenteral administration. J Am Vet Med Assoc. 2007;230(2):233-243. doi:10.2460\/javma.230.2.233"},{"issue":"9-10","key":"ref20","series-title":"Vaccine","first-page":"907","article-title":"The efficacy of modified-live bovine respiratory syncytial virus vaccines in experimentally infected calves","volume":"18","author":"West K","year":"1999","unstructured":"West K, Petrie L, Konoby C, Haines DM, Cortese V, Ellis JA. The efficacy of modified-live bovine respiratory syncytial virus vaccines in experimentally infected calves. Vaccine. 1999;18(9-10):907-919. doi:10.1016\/s0264-410x(99)00324-2"},{"issue":"12","key":"ref21","series-title":"J Am Vet Med Assoc","first-page":"1973","article-title":"Efficacy of an inactivated respiratory syncytial virus vaccine in calves","volume":"218","author":"Ellis J","year":"2001","unstructured":"Ellis J, West K, Konoby C,  Efficacy of an inactivated respiratory syncytial virus vaccine in calves. J Am Vet Med Assoc. 2001;218(12):1973-1980. doi:10.2460\/javma.2001.218.1973"},{"issue":"12","key":"ref22","series-title":"Can Vet J","first-page":"1311","article-title":"Comparative efficacy of modified-live and inactivated vaccines in boosting responses to bovine respiratory syncytial virus following neonatal mucosal priming of beef calves","volume":"59","author":"Ellis J","year":"2018","unstructured":"Ellis J, Gow S, Berenik A, Lacoste S, Erickson N. Comparative efficacy of modified-live and inactivated vaccines in boosting responses to bovine respiratory syncytial virus following neonatal mucosal priming of beef calves. Can Vet J. 2018;59(12):1311-1319."},{"issue":"4","key":"ref23","series-title":"Can Vet J","first-page":"321","article-title":"Comparative efficacy of modified-live and inactivated vaccines in boosting disease-sparing responses to bovine viral diarrhea virus challenge in neonatally mucosally primed weanling beef calves","volume":"64","author":"Erickson N","year":"2023","unstructured":"Erickson N, Godson D, Gow S, Lacoste S, Waldner C, Ellis J. Comparative efficacy of modified-live and inactivated vaccines in boosting disease-sparing responses to bovine viral diarrhea virus challenge in neonatally mucosally primed weanling beef calves. Can Vet J. 2023;64(4):321-328."},{"issue":"5","key":"ref24","series-title":"Can Vet J","first-page":"463","article-title":"A comparison of 2 vaccination programs in feedlot calves at ultra-high risk of developing undifferentiated fever\/bovine respiratory disease","volume":"49","author":"Wildman BK","year":"2008","unstructured":"Wildman BK, Perrett T, Abutarbush SM,  A comparison of 2 vaccination programs in feedlot calves at ultra-high risk of developing undifferentiated fever\/bovine respiratory disease. Can Vet J. 2008;49(5):463-472."},{"key":"ref25","series-title":"Bov Pract","first-page":"23","article-title":"A comparison of two multivalent modified live viral\/bacterial combination vaccines in feedlot calves at ultra-high risk of developing undifferentiated fever\/bovine respiratory disease","volume":"58","author":"Hunsaker BD","year":"2024","unstructured":"Hunsaker BD, McMullen CA, Stamm CG,  A comparison of two multivalent modified live viral\/bacterial combination vaccines in feedlot calves at ultra-high risk of developing undifferentiated fever\/bovine respiratory disease. Bov Pract. 2024;58:23-33. doi:10.21423\/bpj20249018"},{"issue":"10","key":"ref26","series-title":"Am J Respir Crit Care Med","first-page":"1305","article-title":"Viral load drives disease in humans experimentally infected with respiratory syncytial virus","volume":"182","author":"DeVincenzo JP","year":"2010","unstructured":"DeVincenzo JP, Wilkinson T, Vaishnaw A,  Viral load drives disease in humans experimentally infected with respiratory syncytial virus. Am J Respir Crit Care Med. 2010;182(10):1305-1314. doi:10.1164\/rccm.201002-0221OC"},{"issue":"16","key":"ref27","series-title":"N Engl J Med","first-page":"1465","article-title":"Efficacy and safety of a bivalent RSV prefusion F vaccine in older adults","volume":"388","author":"Walsh EE","year":"2023","unstructured":"Walsh EE, P\u00e9rez Marc G, Zareba AM,  Efficacy and safety of a bivalent RSV prefusion F vaccine in older adults. N Engl J Med. 2023;388(16):1465-1477. doi:10.1056\/NEJMoa2213836"},{"issue":"10","key":"ref28","series-title":"J Am Vet Med Assoc","first-page":"1119","article-title":"Innate and acquired immune responses of colostrum-fed neonatal Holstein calves following intranasal vaccination with two commercially available modified-live virus vaccines","volume":"258","author":"Midla LT","year":"2021","unstructured":"Midla LT, Hill KL, Van Engen NK,  Innate and acquired immune responses of colostrum-fed neonatal Holstein calves following intranasal vaccination with two commercially available modified-live virus vaccines. J Am Vet Med Assoc. 2021;258(10):1119-1129. doi:10.2460\/javma.258.10.1119"}],"container-title":["Journal of the American Veterinary Medical Association"],"original-title":[],"link":[{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/javma\/264\/11\/javma.26.04.0247.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/javma\/264\/11\/javma.26.04.0247.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/javma\/264\/11\/javma.26.04.0247.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:25:18Z","timestamp":1792171518000},"score":1,"resource":{"primary":{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/javma\/264\/11\/javma.26.04.0247.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11,1]]},"references-count":28,"journal-issue":{"issue":"11"},"URL":"https:\/\/doi.org\/10.2460\/javma.26.04.0247","relation":{},"ISSN":["0003-1488"],"issn-type":[{"value":"0003-1488","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11,1]]}}}