{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T13:05:21Z","timestamp":1790168721298,"version":"4.1.0"},"reference-count":4,"publisher":"American Veterinary Medical Association (AVMA)","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["javma"],"published-print":{"date-parts":[[2026,9,23]]},"abstract":"Abstract<\/jats:title>\n \n Objective<\/jats:title>\n To demonstrate the technique of gene electrotransfer (GET) for administration of plasmid DNA immunotherapy in veterinary oncology.<\/jats:p>\n <\/jats:sec>\n \n Animals<\/jats:title>\n The method demonstrated in the video is relevant to both canine and feline cancer patients undergoing administration of DNA immunotherapies.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n This Technical Tutorial Video demonstrates patient preparation, IM plasmid administration, electrode positioning, electroporation pulse delivery, and postprocedural assessment for a GET procedure. Required equipment, procedure workflow, and practical technical considerations are reviewed.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n The video illustrates the general workflow for delivery of plasmid DNA immunotherapy using IM GET under general anesthesia, including patient preparation, plasmid administration, pulse delivery, and patient monitoring.<\/jats:p>\n <\/jats:sec>\n \n Clinical Relevance<\/jats:title>\n GET is increasingly used in veterinary oncology to facilitate delivery of DNA immunotherapies targeting tumor-associated antigens and immunomodulatory molecules. This tutorial provides a practical overview of the technique.<\/jats:p>\n <\/jats:sec>","DOI":"10.2460\/javma.26.05.0428","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T12:24:51Z","timestamp":1790166291000},"page":"1","source":"Crossref","is-referenced-by-count":0,"title":["Gene electrotransfer for DNA immunotherapy in veterinary oncology: Technical Tutorial Video"],"prefix":"10.2460","author":[{"given":"Joseph A.","family":"Impellizeri","sequence":"first","affiliation":[{"name":"Veterinary Oncology Services, New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arbel","family":"Vigodny","sequence":"additional","affiliation":[{"name":"Veterinary Oncology Services, New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Sanchez","sequence":"additional","affiliation":[{"name":"Veterinary Oncology Services, New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3110-4534","authenticated-orcid":true,"given":"Luigi","family":"Aurisicchio","sequence":"additional","affiliation":[{"name":"Evvivax Biotech, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Conforti","sequence":"additional","affiliation":[{"name":"Evvivax Biotech, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"issue":"4","key":"ref1","series-title":"Vet Comp Oncol","first-page":"310","article-title":"Electro-gene-transfer as a new tool for cancer immunotherapy in animals","volume":"12","author":"Impellizeri JA","year":"2014","unstructured":"Impellizeri JA, Ciliberto G, Aurisicchio L. Electro-gene-transfer as a new tool for cancer immunotherapy in animals. Vet Comp Oncol. 2014;12(4):310-318. doi:10.1111\/vco.12006"},{"key":"ref2","series-title":"Mol Ther Methods Clin Dev","first-page":"238","article-title":"A linear DNA encoding the SARS-CoV-2 receptor binding domain elicits potent immune response and neutralizing antibodies in domestic cats","volume":"28","author":"Conforti A","year":"2023","unstructured":"Conforti A, Sanchez E, Salvatori E, A linear DNA encoding the SARS-CoV-2 receptor binding domain elicits potent immune response and neutralizing antibodies in domestic cats. Mol Ther Methods Clin Dev. 2023;28:238-248. doi:10.1016\/j.omtm.2022.12.015"},{"issue":"2","key":"ref3","series-title":"Curr Gene Ther","first-page":"98","article-title":"Gene electrotransfer: a mechanistic perspective","volume":"16","author":"Rosazza C","year":"2016","unstructured":"Rosazza C, Meglic SH, Zumbusch A, Rols MP, Miklavcic D. Gene electrotransfer: a mechanistic perspective. Curr Gene Ther. 2016;16(2):98-129. doi:10.2174\/1566523216666160331130040"},{"key":"ref4","series-title":"Int Immunopharmacol","first-page":"110274","article-title":"Treatment of spontaneous canine mast cell tumors by electrochemotherapy combined with IL-12 gene electrotransfer: comparison of intratumoral and peritumoral application of IL-12","volume":"120","author":"Lampreht Tratar U","year":"2023","unstructured":"Lampreht Tratar U, Milevoj N, Cemazar M, Treatment of spontaneous canine mast cell tumors by electrochemotherapy combined with IL-12 gene electrotransfer: comparison of intratumoral and peritumoral application of IL-12. Int Immunopharmacol. 2023;120:110274. doi:10.1016\/j.intimp.2023.110274"}],"container-title":["Journal of the American Veterinary Medical Association"],"original-title":[],"link":[{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/javma\/aop\/javma.26.05.0428\/javma.26.05.0428.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/javma\/aop\/javma.26.05.0428\/javma.26.05.0428.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T12:24:51Z","timestamp":1790166291000},"score":1,"resource":{"primary":{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/javma\/aop\/javma.26.05.0428\/javma.26.05.0428.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,23]]},"references-count":4,"URL":"https:\/\/doi.org\/10.2460\/javma.26.05.0428","relation":{},"ISSN":["0003-1488"],"issn-type":[{"value":"0003-1488","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9,23]]}}}