{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T09:31:45Z","timestamp":1777282305141,"version":"3.51.4"},"reference-count":9,"publisher":"Walter de Gruyter GmbH","issue":"6","license":[{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Dengue virus infection is an intriguing illness. It is traditionally thought of as a self-limited and nonpersistent disease.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>We report a case with persistent dengue virus genome detectable in hematopoietic cells of a person with remote infection.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A patient with multiple myeloma in remission was prepared for peripheral blood stem cell (PBSC) transplantation. Plasma and G-CSF-stimulated, mobilized PBSCs were collected. Dengue-specific reverse transcription polymerase chain reaction (RT-PCR) was performed in both pre- and post-stimulated blood specimens. Anti-dengue antibodies by ELISA and by neutralization assay were measured before and after the stem cell mobilization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The viral genome was detected only in the PBSC of the post-G-CSF-stimulated specimens. Anti-dengue antibodies were negative and positive, by ELISA and neutralization assays, respectively, both before and after stem cell mobilization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings reveal a persistent infection. Whether and how this strain may interact with subsequent serotype(s) remains to be elucidated.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.2478\/abm-2023-0072","type":"journal-article","created":{"date-parts":[[2023,12,28]],"date-time":"2023-12-28T14:51:42Z","timestamp":1703775102000},"page":"287-290","source":"Crossref","is-referenced-by-count":2,"title":["Persistence of dengue genome in a remotely infected patient"],"prefix":"10.2478","volume":"17","author":[{"given":"Soraya","family":"Thaivanich","sequence":"first","affiliation":[{"name":"Clinical Excellence Center for Emerging Infectious Diseases , King Chulalongkorn Memorial Hospital , Thai Red Cross Society , Bangkok , Thailand"},{"name":"Department of Medicine, Faculty of Medicine , Chulalongkorn University , Bangkok , Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jirayu","family":"Visuthranukul","sequence":"additional","affiliation":[{"name":"Clinical Excellence Center for Emerging Infectious Diseases , King Chulalongkorn Memorial Hospital , Thai Red Cross Society , Bangkok , Thailand"},{"name":"Department of Medicine, Faculty of Medicine , Chulalongkorn University , Bangkok , Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kesinee","family":"Arunyingmongkol","sequence":"additional","affiliation":[{"name":"Clinical Excellence Center for Emerging Infectious Diseases , King Chulalongkorn Memorial Hospital , Thai Red Cross Society , Bangkok , Thailand"},{"name":"Department of Medicine, Faculty of Medicine , Chulalongkorn University , Bangkok , Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Udomsak","family":"Bunworasate","sequence":"additional","affiliation":[{"name":"Department of Medicine, Faculty of Medicine , Chulalongkorn University , Bangkok , Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Padet","family":"Siriyasatien","sequence":"additional","affiliation":[{"name":"Clinical Excellence Center for Emerging Infectious Diseases , King Chulalongkorn Memorial Hospital , Thai Red Cross Society , Bangkok , Thailand"},{"name":"Department of Parasitology, Faculty of Medicine , Chulalongkorn University , Bangkok , Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanla","family":"Kulwichit","sequence":"additional","affiliation":[{"name":"Clinical Excellence Center for Emerging Infectious Diseases , King Chulalongkorn Memorial Hospital , Thai Red Cross Society , Bangkok , Thailand"},{"name":"Department of Medicine, Faculty of Medicine , Chulalongkorn University , Bangkok , Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2023,12,28]]},"reference":[{"key":"2026042708193137175_j_abm-2023-0072_ref_001","doi-asserted-by":"crossref","unstructured":"Simmons CP, Farrar JJ, Nguyen vV, Wills B. Dengue. N Engl J Med. 2012; 366:1423\u201332.","DOI":"10.1056\/NEJMra1110265"},{"key":"2026042708193137175_j_abm-2023-0072_ref_002","doi-asserted-by":"crossref","unstructured":"Putcharoen P, Krajiw S, Nilaratanakul V, Rojnuckarin P, Bhattarakosol P, Nisalak A, et al. Presence of dengue virus genome in the bone marrow of asymptomatic adults in a dengue-hyperendemic country: implication for complicated dengue pathogenesis. Clin Microbiol Infect. 2007; 13(S1):S14.","DOI":"10.1016\/S0924-8579(07)70047-X"},{"key":"2026042708193137175_j_abm-2023-0072_ref_003","doi-asserted-by":"crossref","unstructured":"Innis BL, Nisalak A, Nimmannitya S, Kusalerdchariya S, Chongswasdi V, Suntayakorn S, et al. An enzyme-linked immunosorbent assay to characterize dengue infections where dengue and Japanese encephalitis co-circulate. Am J Trop Med Hyg. 1989; 40:418\u201327.","DOI":"10.4269\/ajtmh.1989.40.418"},{"key":"2026042708193137175_j_abm-2023-0072_ref_004","unstructured":"Okuno Y, Igarashi A, Fukai K. Neutralization tests for dengue and Japanese encephalitis viruses by the focus reduction method using peroxidase-anti-peroxidase staining. Biken J. 1978; 21:137\u201347."},{"key":"2026042708193137175_j_abm-2023-0072_ref_005","doi-asserted-by":"crossref","unstructured":"Lanciotti RS, Calisher CH, Gubler DJ, Chang GJ, Vorndam AV. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J Clin Microbiol. 1992; 30:545\u201351.","DOI":"10.1128\/jcm.30.3.545-551.1992"},{"key":"2026042708193137175_j_abm-2023-0072_ref_006","doi-asserted-by":"crossref","unstructured":"Kwok S, Higuchi R. Avoiding false positives with PCR. Nature. 1989; 339:237\u20138.","DOI":"10.1038\/339237a0"},{"key":"2026042708193137175_j_abm-2023-0072_ref_007","doi-asserted-by":"crossref","unstructured":"Murray K, Walker C, Herrington E, Lewis JA, McCormick J, Beasley DW, et al. Persistent infection with West Nile virus years after initial infection. J Infect Dis. 2010; 201:2\u20134.","DOI":"10.1086\/648731"},{"key":"2026042708193137175_j_abm-2023-0072_ref_008","unstructured":"Kulwichit W, Mekmullica J, Krajiw S, Prommalikit O, Yapom R, Intaraprasong P, et al. Highly-sensitive virologic diagnosis of dengue infection by a newly-developed protocol of reverse transcription-nested polymerase chain reaction (RT-Nested PCR) of serum\/plasma, peripheral blood leukocyte (PBL), and urine specimens. The 41st Annual Meeting of the Infectious Diseases Society of America; 2003 October 9\u201312, San Diego, USA. 2003."},{"key":"2026042708193137175_j_abm-2023-0072_ref_009","doi-asserted-by":"crossref","unstructured":"Perret C, Chanthavanich P, Pengsaa K, Limkittikul K, Hutajaroen P, Bunn JEG, Brabin BJ. Dengue infection during pregnancy and transplacental antibody transfer in Thai mothers. J Infect. 2005; 51:287\u201393.","DOI":"10.1016\/j.jinf.2004.10.003"}],"container-title":["Asian Biomedicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/reference-global.com\/pdf\/10.2478\/abm-2023-0072","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T08:31:42Z","timestamp":1777278702000},"score":1,"resource":{"primary":{"URL":"https:\/\/reference-global.com\/article\/10.2478\/abm-2023-0072"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,1]]},"references-count":9,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,12,28]]},"published-print":{"date-parts":[[2023,12,1]]}},"alternative-id":["10.2478\/abm-2023-0072"],"URL":"https:\/\/doi.org\/10.2478\/abm-2023-0072","relation":{},"ISSN":["1875-855X"],"issn-type":[{"value":"1875-855X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,1]]}}}