{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T19:22:04Z","timestamp":1785439324487,"version":"3.56.0"},"reference-count":26,"publisher":"Walter de Gruyter GmbH","issue":"4","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Preeclampsia (PE) is a complex pregnancy disorder that may cause adverse outcomes for mother and baby. Combining risk factors with clinical, laboratory, and ultrasonographic data can help identify women at risk. This study investigated the relationship between maternal risk factors, soluble fms-like tyro-sine kinase 1 (sFlt-1), placental growth factor (PlGF), their ratio, and pregnancy outcomes, involving 68 women with PE risk factors and 21 controls. There were no significant differences in the frequency of adverse outcomes (PE, foetal death, and infants with abnormal birth weight), sFlt-1, PlGF, the sFlt-1\/PlGF ratio, or birth weight centiles between the PE-risk and control groups. The most frequently recorded high-risk factor was gestational diabetes mellitus, whereas moderate risk was a pre-pregnancy body mass index of over 30 kg m\n                    <jats:sup>\u20132<\/jats:sup>\n                    . The most prominent difference was observed in the subgroups with gestational hypertension and first-time pregnant women as risk factors, with significantly higher sFlt-1\/PlGF ratios compared to the control group. Combining multiple risk factors increased the sFlt-1\/PlGF ratio compared to both the control group and the group with only one risk factor. The study documented PE risk factors and outcomes at a Croatian hospital where angiogenic markers are not routinely used in screening. Findings highlighted the importance of integrating PE screening into standard practice.\n                  <\/jats:p>","DOI":"10.2478\/acph-2025-0040","type":"journal-article","created":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T11:03:29Z","timestamp":1768302209000},"page":"687-697","source":"Crossref","is-referenced-by-count":1,"title":["Investigating preeclampsia risk factors and angiogenic profiles in low-screening area"],"prefix":"10.2478","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0795-3547","authenticated-orcid":false,"given":"Adriana","family":"Bokuli\u0107","sequence":"first","affiliation":[{"name":"Sestre Milosrdnice University Hospital Center , Department of Clinical Chemistry , Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1863-8198","authenticated-orcid":false,"given":"Dra\u017ean","family":"Butorac","sequence":"additional","affiliation":[{"name":"Sestre Milosrdnice University Hospital Center , Clinic of Gynaecology and Obstetrics Zagreb , Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2275-6389","authenticated-orcid":false,"given":"Ivka","family":"Djakovi\u0107","sequence":"additional","affiliation":[{"name":"Bethesda Hospital, Clinic for Gynaecology , Basel , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6332-0667","authenticated-orcid":false,"given":"Marijo","family":"\u010cukelj","sequence":"additional","affiliation":[{"name":"Sestre Milosrdnice University Hospital Center , Clinic of Gynaecology and Obstetrics Zagreb , Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7054-3656","authenticated-orcid":false,"given":"Marija Grdi\u0107","family":"Rajkovi\u0107","sequence":"additional","affiliation":[{"name":"University of Zagreb Faculty of Pharmacy and Biochemistry , Department of Medical Biochemistry and Haematology , Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2026,1,13]]},"reference":[{"key":"2026062413352478324_j_acph-2025-0040_ref_001","doi-asserted-by":"crossref","unstructured":"B. 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Tsigas and P. von Dadelszen, The 2021 International Society for the Study of Hypertension in Pregnancy classification, diagnosis & management recommendations for international practice, Pregnancy Hypertension 27 (2022) 148\u2013169; https:\/\/doi.org\/10.1016\/j.preghy.2021.09.008","DOI":"10.1016\/j.preghy.2021.09.008"},{"key":"2026062413352478324_j_acph-2025-0040_ref_006","doi-asserted-by":"crossref","unstructured":"E. Dimitriadis, D. L. Rolnik, W. Zhou, G. Estrada-Gutierrez, K. Koga, R. P. V. Francisco, C. White-head, J. Hyett, F. Da Silva Costa, K. Nicolaides and E. Menkhorst, Pre-eclampsia, Nat. Rev. Dis. Primers 9(1) (2023) Article ID 8 (22 pages); https:\/\/doi.org\/10.1038\/s41572-023-00417-6","DOI":"10.1038\/s41572-023-00417-6"},{"key":"2026062413352478324_j_acph-2025-0040_ref_007","doi-asserted-by":"crossref","unstructured":"C. W. Ives, R. Sinkey, I. Rajapreyar, A. T. N. Tita and S. Oparil, Preeclampsia \u2013 pathophysiology and clinical presentations, J. Am. 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Snell, C. Chan, L. C. Chappell, R. Hooper, M. Green, B. W. Mol, B. Thilaganathan, S. Thangaratinam, and on behalf of the IPPIC Network, Prediction of pre-eclampsia: Review of reviews, Ultrasound Obstet. Gynecol. 54(1) (2019) 16\u201327; https:\/\/doi.org\/10.1002\/uog.20117","DOI":"10.1002\/uog.20117"},{"key":"2026062413352478324_j_acph-2025-0040_ref_015","doi-asserted-by":"crossref","unstructured":"K. Duckitt and D. Harrington, Risk factors for pre-eclampsia at antenatal booking: Systematic review of controlled studies, BMJ 330(7491) (2005) Article ID 565 (7 pages); https:\/\/doi.org\/10.1136\/bmj.38380.674340.E0","DOI":"10.1136\/bmj.38380.674340.E0"},{"key":"2026062413352478324_j_acph-2025-0040_ref_016","doi-asserted-by":"crossref","unstructured":"E. Bartsch, K. E. Medcalf, A. L. Park and J. G. 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Graupner, Performance of sFlt-1\/PIGF ratio for the prediction of perinatal outcome in obese pre-eclamptic women, JCM 11(11) (2022) Article ID 3023 (13 pages); https:\/\/doi.org\/10.3390\/jcm11113023","DOI":"10.3390\/jcm11113023"},{"key":"2026062413352478324_j_acph-2025-0040_ref_021","doi-asserted-by":"crossref","unstructured":"G. Dekker and B. Sibai, Primary, secondary, and tertiary prevention of pre-eclampsia, The Lancet 357(9251) (2001) 209\u2013215; https:\/\/doi.org\/10.1016\/S0140-6736(00)03599-6","DOI":"10.1016\/S0140-6736(00)03599-6"},{"key":"2026062413352478324_j_acph-2025-0040_ref_022","doi-asserted-by":"crossref","unstructured":"R. Skj\u00e6rven, A. J. Wilcox and R. T. Lie, The interval between pregnancies and the risk of preeclampsia, N. Engl. J. Med. 346(1) (2002) 33\u201338; https:\/\/doi.org\/10.1056\/NEJMoa011379","DOI":"10.1056\/NEJMoa011379"},{"key":"2026062413352478324_j_acph-2025-0040_ref_023","doi-asserted-by":"crossref","unstructured":"Y. Bdolah, U. Elchalal, S. 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