{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T00:50:43Z","timestamp":1777855843347,"version":"3.51.4"},"reference-count":9,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Although juvenile idiopathic arthritis (JIA) is the most common form of autoimmune-driven arthritis in pediatric population, due to the lack of universal diagnostic markers and heterogeneity of the disease, many patients face a significant delay when establishing the diagnosis. As the hallmark of JIA is the inflammation of synovial membrane with pathological angiogenesis, this study aimed to compare the concentrations of semaphorin 5A (SEMA5A), protein involved in both angiogenesis and immune response, between new-onset JIA and healthy controls. Thirty-five JIA patients and 35 sex-and-age matched healthy controls were enrolled in this study. Serum concentrations of SEMA5A and vascular endothelial growth factor A (VEGF-A) were established using the enzyme-linked immunosorbent assay (ELISA) method. The serum concentration of SEMA5A was elevated in JIA patients in comparison to healthy controls with the median value of 2.04 (interquartile range [IQR]: 12.41) in JIA patients and 1.34 (IQR: 1.79) in healthy controls (\n                    <jats:italic>p<\/jats:italic>\n                    = 0.002). The difference was particularly prominent in oligoarticular JIA, where median SEMA5A concentration equaled 1.76 ng\/mL (IQR: 1.56) in comparison to 0.33 ng\/mL (IQR: 1.34) in sex-and-age-matched healthy controls (\n                    <jats:italic>p<\/jats:italic>\n                    = 0.001). SEMA5A concentration correlated strongly with VEGF-A concentration (\n                    <jats:italic>r<\/jats:italic>\n                    = 0.807,\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.001) and differed significantly in subgroups of different synovial membrane power-Doppler ultrasound (PDUS) inflammatory activities (\n                    <jats:italic>p<\/jats:italic>\n                    = 0.018). Although our findings need to be repeated on larger groups of JIA patients, SEMA5A is a promising marker of JIA, linked to pathological angiogenesis of synovial membrane in this entity. Moreover, it may be useful as a target of innovative treatment strategies in the nearest future.\n                  <\/jats:p>","DOI":"10.2478\/aite-2025-0013","type":"journal-article","created":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T21:32:54Z","timestamp":1748554374000},"source":"Crossref","is-referenced-by-count":0,"title":["Semaphorin 5A as a Novel Diagnostic Biomarker of Juvenile Idiopathic Arthritis"],"prefix":"10.2478","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1487-7156","authenticated-orcid":false,"given":"Justyna","family":"Roszkiewicz","sequence":"first","affiliation":[{"name":"Department of Pediatric Cardiology and Rheumatology , Medical University of Lodz , Lodz , Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6599-8188","authenticated-orcid":false,"given":"Krystyna","family":"Wyka","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Hematology and Oncology , Medical University of Lodz , Lodz , Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8421-0448","authenticated-orcid":false,"given":"El\u017cbieta","family":"Smolewska","sequence":"additional","affiliation":[{"name":"Department of Pediatric Cardiology and Rheumatology , Medical University of Lodz , Lodz , Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2025,5,28]]},"reference":[{"key":"2026042921034469751_j_aite-2025-0013_ref_001","doi-asserted-by":"crossref","unstructured":"Cheng Q, Chen M, Liu M et al. (2022) Semaphorin 5A suppresses ferroptosis through activation of PI3K-AKT-mTOR signaling in rheumatoid arthritis. Cell Death Dis 13:608. https:\/\/doi.org\/10.1038\/s41419-022-05065-4","DOI":"10.1038\/s41419-022-05065-4"},{"key":"2026042921034469751_j_aite-2025-0013_ref_002","doi-asserted-by":"crossref","unstructured":"Chen K, Zeng H, Togizbayev G et al. (2023) New classification criteria for juvenile idiopathic arthritis. Int J Rheum Dis 26:1889\u20131892. https:\/\/doi.org\/10.1111\/1756-185X.14813","DOI":"10.1111\/1756-185X.14813"},{"key":"2026042921034469751_j_aite-2025-0013_ref_003","doi-asserted-by":"crossref","unstructured":"Gras C, Eiz-Vesper B, Jaimes Y et al. (2014) Secreted semaphorin 5A activates immune effector cells and is a biomarker for rheumatoid arthritis. Arthritis Rheumatol 66:1461\u20131471. https:\/\/doi.org\/10.1002\/art.38425","DOI":"10.1002\/art.38425"},{"key":"2026042921034469751_j_aite-2025-0013_ref_004","unstructured":"Petty RE, Southwood TR, Manners P et al. (2004) International league of associations for rheumatology classification of juvenile idiopathic arthritis: Second revision, Edmonton, 2001. J Rheumatol 31:390\u2013392. PMID: 14760812."},{"key":"2026042921034469751_j_aite-2025-0013_ref_005","doi-asserted-by":"crossref","unstructured":"Qin Y, Jin J, Zhang J et al. (2023) A fully human monoclonal antibody targeting Semaphorin 5A alleviates the progression of rheumatoid arthritis. Biomed Pharmacother 168:115666. https:\/\/doi.org\/10.1016\/j.biopha.2023.115666","DOI":"10.1016\/j.biopha.2023.115666"},{"key":"2026042921034469751_j_aite-2025-0013_ref_006","doi-asserted-by":"crossref","unstructured":"Sadanandam A, Rosenbaugh EG, Singh S et al. (2010) Semaphorin 5A promotes angiogenesis by increasing endothelial cell proliferation, migration, and decreasing apoptosis. Microvasc Res 79:1\u20139. https:\/\/doi.org\/10.1016\/j.mvr.2009.10.005","DOI":"10.1016\/j.mvr.2009.10.005"},{"key":"2026042921034469751_j_aite-2025-0013_ref_007","doi-asserted-by":"crossref","unstructured":"\u015awidrowska-Jaros J, Smolewska E (2018) A fresh look at angiogenesis in juvenile idiopathic arthritis. Centr Eur J Immunol 43:325\u2013330. https:\/\/doi.org\/10.5114\/ceji.2018.80052","DOI":"10.5114\/ceji.2018.80052"},{"key":"2026042921034469751_j_aite-2025-0013_ref_008","doi-asserted-by":"crossref","unstructured":"Xiao C, Lv C, Sun S et al. (2021) TSP1 is the essential domain of SEMA5A involved in pannus formation in rheumatoid arthritis. Rheumatology 60:5833\u20135842. https:\/\/doi.org\/10.1093\/rheumatology\/keab133","DOI":"10.1093\/rheumatology\/keab133"},{"key":"2026042921034469751_j_aite-2025-0013_ref_009","doi-asserted-by":"crossref","unstructured":"Zaripova LN, Midgley A, Christmas SE et al. (2021) Juvenile idiopathic arthritis: From aetiopathogenesis to therapeutic approaches. Pediatr Rheumatol Online J 19:135. https:\/\/doi.org\/10.1186\/s12969-021-00629-8","DOI":"10.1186\/s12969-021-00629-8"}],"container-title":["Archivum Immunologiae et Therapiae Experimentalis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/reference-global.com\/pdf\/10.2478\/aite-2025-0013","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T23:31:17Z","timestamp":1777505477000},"score":1,"resource":{"primary":{"URL":"https:\/\/reference-global.com\/article\/10.2478\/aite-2025-0013"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,1]]},"references-count":9,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,5,28]]},"published-print":{"date-parts":[[2025,1,1]]}},"alternative-id":["10.2478\/aite-2025-0013"],"URL":"https:\/\/doi.org\/10.2478\/aite-2025-0013","relation":{},"ISSN":["1661-4917"],"issn-type":[{"value":"1661-4917","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,1]]},"article-number":"20250013"}}