{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T23:01:21Z","timestamp":1785020481624,"version":"3.55.0"},"reference-count":42,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Acute pancreatitis (AP) frequently triggers intestinal barrier dysfunction, yet the underlying molecular mechanisms remain poorly understood. This study investigated whether small extracellular vesicle (sEV)-enriched serum preparations from healthy individuals protect against AP-induced intestinal barrier dysfunction through microRNA-mediated regulation of pyroptosis. sEV-enriched preparations were isolated from 10 AP patients (AP-sEV) and 10 healthy controls (HC-sEV) using ExoQuick precipitation followed by ultracentrifugation. A murine AP model was established using cerulein and lipopolysaccharide, and human intestinal epithelial cells were stimulated with lipopolysaccharide for\n                    <jats:italic>in vitro<\/jats:italic>\n                    studies. HC-sEV preparations significantly ameliorated AP-induced tissue damage, restored tight junction proteins (claudin-1, occludin, ZO-1), reduced intestinal permeability, and suppressed inflammatory cytokines (TNF-\u03b1, IL-6, IL-1\u03b2) and pyroptosis-related proteins including NLRP3, gasdermin D, and cleaved caspase-1. Conversely, AP-sEV preparations exacerbated barrier dysfunction. Bioinformatics analysis identified miR-579-3p as significantly downregulated in AP-sEV. Inhibition of miR-579-3p reversed HC-sEV protective effects. ANXA3 was validated as a direct miR-579-3p target, and ANXA3 overexpression counteracted miR-579-3p-mediated protection. These findings demonstrate that HC-sEV preparations protect against AP-induced intestinal barrier dysfunction through miR-579-3p delivery targeting ANXA3 to suppress NLRP3 inflammasome-mediated pyroptosis. The miR-579-3p\/ANXA3\/NLRP3 axis represents a novel therapeutic target for preserving intestinal barrier integrity during acute pancreatitis.\n                  <\/jats:p>","DOI":"10.2478\/aite-2026-0018","type":"journal-article","created":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T13:58:00Z","timestamp":1782395880000},"source":"Crossref","is-referenced-by-count":0,"title":["sEV-Enriched Serum Preparations From Healthy Individuals Protect Against Acute Pancreatitis-Induced Intestinal Barrier Dysfunction Through miR-579-3p\/ANXA3-Mediated Inhibition of NLRP3 Inflammasome Pyroptosis"],"prefix":"10.2478","volume":"74","author":[{"given":"Huiyuan","family":"Gu","sequence":"first","affiliation":[{"name":"Department of Gastroenterology , The First Affiliated Hospital of Soochow University , Suzhou , Jiangsu Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenyue","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology , The First Affiliated Hospital of Soochow University , Suzhou , Jiangsu Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqi","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology , The First Affiliated Hospital of Soochow University , Suzhou , Jiangsu Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqing","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology , The First Affiliated Hospital of Soochow University , Suzhou , Jiangsu Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunfang","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology , The First Affiliated Hospital of Soochow University , Suzhou , Jiangsu Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2026,6,25]]},"reference":[{"key":"2026072522431477105_j_aite-2026-0018_ref_001","doi-asserted-by":"crossref","unstructured":"Bei Y, Chen T, Banciu DD et al. 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