{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T23:01:20Z","timestamp":1785020480926,"version":"3.55.0"},"reference-count":18,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Fracture-related infection is a challenging aspect of orthopedic care, as it leads to prolonged inflammation and impaired bone healing. Although isovitexin is a naturally occurring flavonoid with anti-inflammatory properties, its effects on fracture-related infection remain unclear. A rat fracture infection model was established and randomly divided into four groups: control, model, Vancomycin, and isovitexin. Tissue staining was used to assess bone healing. In contrast, enzyme-linked immunosorbent assay and biochemical kits were used to measure the levels of inflammatory factors and bone metabolism-related indicators. The expression of proteins associated with osteogenesis and the Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-\u03baB) signaling pathway was examined using Western blot. When compared to the model group, isovitexin treatment more effectively reduced inflammatory cell infiltration in bone tissue and promoted callus formation than the Vancomycin group. In addition, isovitexin improved the imbalance of bone metabolism and promoted the expression of osteogenesis-related proteins Bone Morphogenetic Protein 2 (BMP2), Osteopontin (OPN), and Runt-related Transcription Factor 2 (RUNX2). It also decreased the levels of pro-inflammatory factors tumor necrosis factor (TNF)-\u03b1 and interleukin (IL)-6, and increased the level of the anti-inflammatory factor IL-10. Mechanistic studies showed that isovitexin significantly inhibited the activation of the NF-\u03baB signaling pathway. Isovitexin can promote bone metabolism and healing, suppress the NF-\u03baB signaling pathway, and reduce the inflammatory response associated with fracture-related infection. This study suggests that isovitexin may be a viable therapy option for fracture-related infections.<\/jats:p>","DOI":"10.2478\/aite-2026-0021","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T17:58:16Z","timestamp":1783965496000},"source":"Crossref","is-referenced-by-count":0,"title":["Isovitexin can Alleviate Fracture-Related Infection, Promote Osteogenesis, and Inhibit Activation of the NF-\u03baB Signaling Pathway"],"prefix":"10.2478","volume":"74","author":[{"given":"Yaping","family":"Gu","sequence":"first","affiliation":[{"name":"Disinfection Supply Center, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine , Suzhou , Jiangsu , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine , Suzhou , Jiangsu , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2026,7,13]]},"reference":[{"key":"2026072522431279851_j_aite-2026-0021_ref_001","doi-asserted-by":"crossref","unstructured":"Abdulai IL, Kwofie SK, Gbewonyo WS et al. (2021) Multitargeted effects of vitexin and isovitexin on diabetes mellitus and its complications. Sci World J 2021:6641128. https:\/\/doi.org\/10.1155\/2021\/6641128","DOI":"10.1155\/2021\/6641128"},{"key":"2026072522431279851_j_aite-2026-0021_ref_002","doi-asserted-by":"crossref","unstructured":"Cheng H (2025) Isovitexin: A promising active compound found in nature's bounty. Plant Foods Hum Nutr 80:197. https:\/\/doi.org\/10.1007\/s11130-025-01440-6","DOI":"10.1007\/s11130-025-01440-6"},{"key":"2026072522431279851_j_aite-2026-0021_ref_003","doi-asserted-by":"crossref","unstructured":"Dai Y, Yi X, Huang Y et al. (2024) miR-345-3p modulates M1\/M2 macrophage polarization to inhibit inflammation in bone infection via targeting MAP3K1 and NF-\u03baB pathway. J Immunol 212:844\u2013854. https:\/\/doi.org\/10.4049\/jimmunol.2300561","DOI":"10.4049\/jimmunol.2300561"},{"key":"2026072522431279851_j_aite-2026-0021_ref_004","doi-asserted-by":"crossref","unstructured":"Depypere M, Sliepen J, Onsea J et al. (2022) The microbiological etiology of fracture-related infection. Front Cell Infect Microbiol 12:934485. https:\/\/doi.org\/10.3389\/fcimb.2022.934485","DOI":"10.3389\/fcimb.2022.934485"},{"key":"2026072522431279851_j_aite-2026-0021_ref_005","doi-asserted-by":"crossref","unstructured":"Dvorak JE, Lasinski AM, Romeo NM et al. (2024) Fracture related infection and sepsis in orthopedic trauma: A review. Surgery 176:535\u2013540. https:\/\/doi.org\/10.1016\/j.surg.2024.04.031","DOI":"10.1016\/j.surg.2024.04.031"},{"key":"2026072522431279851_j_aite-2026-0021_ref_006","doi-asserted-by":"crossref","unstructured":"Fan Y, Chen Z, Wang H et al. (2025) Isovitexin targets SIRT3 to prevent steroid-induced osteonecrosis of the femoral head by modulating mitophagy-mediated ferroptosis. Bone Res 13:18. https:\/\/doi.org\/10.1038\/s41413-024-00390-0","DOI":"10.1038\/s41413-024-00390-0"},{"key":"2026072522431279851_j_aite-2026-0021_ref_007","doi-asserted-by":"crossref","unstructured":"Ferdausi N, Islam S, Rimti FH et al. (2022) Point-specific interactions of isovitexin with the neighboring amino acid residues of the hACE2 receptor as a targeted therapeutic agent in suppressing the SARS-CoV-2 influx mechanism. J Adv Vet Anim Res 9:230\u2013240. https:\/\/doi.org\/10.5455\/javar.2022.i588","DOI":"10.5455\/javar.2022.i588"},{"key":"2026072522431279851_j_aite-2026-0021_ref_008","doi-asserted-by":"crossref","unstructured":"Graan D, Balogh ZJ (2022) Microbiology of fracture related infections. 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