{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T19:45:55Z","timestamp":1792007155989,"version":"4.4.0"},"reference-count":20,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    MicroRNAs (miRNAs) are key post-transcriptional regulators of gene expression. Given the dynamic nature of the peripheral blood transcriptome, profiling circulating miRNAs in autism spectrum disorder (ASD) represents a promising strategy for identifying novel disease-associated biomarkers. Large-scale miRNA expression profiling was performed using the \u03bcParaflo\u2122 miRNA microarray assay on pooled RNA samples from 23 individuals with ASD and 21 healthy controls. Differential expression analysis (p &lt; 0.05) identified several dysregulated miRNAs in peripheral blood, including miR-128-3p. Microarray findings for miR-128-3p were validated by quantitative reverse transcription PCR (qRT-PCR), which confirmed its significant downregulation in the ASD group. Target gene analysis using miRWalk indicated that miR-128-3p exerts multilevel regulatory effects across diverse biological processes. Notably, predicted and validated targets included genes involved in neuronal development (e.g.,\n                    <jats:italic>PTEN<\/jats:italic>\n                    ) and epigenetic regulation (e.g.,\n                    <jats:italic>DNMT1, RNASEN, DICER,<\/jats:italic>\n                    and\n                    <jats:italic>EIF2C2<\/jats:italic>\n                    ). Receiver operating characteristic (ROC) curve analysis demonstrated significant diagnostic potential of miR-128-3p for ASD. Collectively, these findings support an integrative model of ASD as a heterogeneous neurodevelopmental disorder in which specific miRNAs, such as miR-128-3p, contribute to disease pathogenesis through modulation of gene expression networks.\n                  <\/jats:p>","DOI":"10.2478\/bjmg-2026-0009","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:57:27Z","timestamp":1792004247000},"page":"7-16","source":"Crossref","is-referenced-by-count":0,"title":["Identification of Peripheral Blood mIR-128-3p Molecular Signature in Children with Autism Spectrum Disorder"],"prefix":"10.2478","volume":"29","author":[{"given":"TN","family":"Popov","sequence":"first","affiliation":[{"name":"Medical Center Spectar \u2013 Plovdiv ; 107 Macedonia Street ; Plovdiv , Bulgaria ."},{"name":"Department of Psychology ; University of Plovdiv \u201cPaisii Hilendarski\u201d , 24 Tzar Asen Street ; Plovdiv , Bulgaria ."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SD","family":"Minchev","sequence":"additional","affiliation":[{"name":"Department of Human Anatomy and Physiology ; University of Plovdiv \u201cPaisii Hilendarski\u201d , 24 Tzar Asen Street ; Plovdiv , Bulgaria ."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TI","family":"Vachev","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology ; University of Plovdiv \u201cPaisii Hilendarski\u201d , 24 Tzar Asen St. ; Plovdiv , Bulgaria ."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"2026101418572342930_j_bjmg-2026-0009_ref_001","doi-asserted-by":"crossref","unstructured":"Abrahams, B.S., & Geschwind, D.H. 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