{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T19:45:58Z","timestamp":1792007158748,"version":"4.4.0"},"reference-count":19,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Background: Maternal overweight and obesity are associated with metabolic, vascular, and obstetric complications. Circulating microRNAs (miRNAs) are stable in plasma and may reflect obesity-related molecular alterations during pregnancy. Aim: To compare plasma miRNA expression profiles between normal-weight and overweight\/obese pregnant women. Methods: The study included 50 pregnant women: 25 with normal weight and 25 with overweight\/obesity. RNA was isolated from individual plasma samples and combined into one pooled RNA sample per study group. The pooled samples were analyzed in technical triplicate using a 96-well miRCURY LNA PCR panel containing 84 target miRNA assays and quality-control\/reference assays. Results: Seventeen target miRNAs met the predefined &gt;2-fold higher-expression threshold and 15 met the &lt;0.5-fold threshold in the over-weight\/obese pool. miR-21-5p was the only target miRNA that met both the predefined twofold magnitude criterion and the nominal p &lt; 0.05 threshold (fold change = 2.58; p = 0.030266). Conclusion: The pooled-sample analysis identifies miR-21-5p as an exploratory candidate signal associated with maternal overweight\/obesity. Individual-sample testing and validation in larger independent cohorts are required before biomarker performance or clinical utility can be established.<\/jats:p>","DOI":"10.2478\/bjmg-2026-0012","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:57:32Z","timestamp":1792004252000},"page":"1-6","source":"Crossref","is-referenced-by-count":0,"title":["Plasma Microrna Profiling in Overweight and Obese Pregnant Women: An Exploratory Pooled-Sample Study"],"prefix":"10.2478","volume":"29","author":[{"given":"V","family":"Spasova","sequence":"first","affiliation":[{"name":"Department of Medical Genetics , Faculty of Medicine, Medical University \u2013 Sofia , Sofia , Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Hristova","sequence":"additional","affiliation":[{"name":"Department of Immunology , National Center of Infectious and Parasitic Diseases , Sofia , Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Rukova","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics , Faculty of Medicine, Medical University \u2013 Sofia , Sofia , Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Koleva","sequence":"additional","affiliation":[{"name":"Clinic of Operative Gynecology , University Hospital \u201cPirogov\u201d , Sofia , Bulgaria"},{"name":"Department of Obstetrics and Gynecology , SofiaMed University Hospital , Sofia , Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Vladimirov","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Medical University \u2013 Sofia , Sofia , Bulgaria"},{"name":"Research Department , SBALAGRM Sofia , Sofia , Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Kolev","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Medical University \u2013 Sofia , Sofia , Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Koleva","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Medical University \u2013 Sofia , Sofia , Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O","family":"Antonova","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics , Faculty of Medicine, Medical University \u2013 Sofia , Sofia , Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Karamishev","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Medical University \u2013 Sofia , Sofia , Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Hadzhidekova","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics , Faculty of Medicine, Medical University \u2013 Sofia , Sofia , Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Karamisheva","sequence":"additional","affiliation":[{"name":"Second Gynecology Clinic, University Hospital \u201cMaichin Dom\u201d , Sofia , Bulgaria"},{"name":"Department of Obstetrics and Gynecology , Faculty of Medicine, Medical University \u2013 Sofia , Sofia , Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"2026101418572430664_j_bjmg-2026-0012_ref_001","unstructured":"World Health Organization. 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