{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T08:54:22Z","timestamp":1777366462210,"version":"3.51.4"},"reference-count":37,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:bold>Objective.<\/jats:bold>\n                    Modified levels of pro- (caspase3, Bax) and anti-apoptotic (Bcl-2) regulatory proteins have been detected in certain brain areas of schizophrenic patients indicating a possible dysregulation of apoptosis. In the present study, effects of antipsychotics, haloperidol (HAL) and olanzapine (OLA), on the gene expression of caspase3 (\n                    <jats:italic>casp3<\/jats:italic>\n                    ),\n                    <jats:italic>Bax<\/jats:italic>\n                    and\n                    <jats:italic>Bcl-2<\/jats:italic>\n                    were studied\n                    <jats:italic>in vitro<\/jats:italic>\n                    in mouse hippocampal mHippoE-2 cell line and\n                    <jats:italic>in vivo<\/jats:italic>\n                    in the hippocampus of MK-801 animal schizophrenia model with the aim to provide evidence that antipsychotics may affect the activity of apoptosis-related markers.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods.<\/jats:bold>\n                    mHippoE-2 cells were incubated with MK-801 (20 \u00b5M), HAL (10 \u00b5M), and OLA (10 \u00b5M) alone or combined, MK-801+HAL\/OLA, for 24, 48, and 72 h. Male Sprague Dawley rats were injected with saline or MK-801 (0.5 mg\/kg) for 6 days and since the 7th day, they were treated with vehicle (VEH), HAL (1 mg\/kg) or OLA (2 mg\/kg) for the next 7 days. The\n                    <jats:italic>casp3<\/jats:italic>\n                    ,\n                    <jats:italic>Bax<\/jats:italic>\n                    and\n                    <jats:italic>Bcl-2<\/jats:italic>\n                    gene expression in mHippoE-2 cells and rat hippocampus was measured by RT-PCR.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results.<\/jats:bold>\n                    In mHippoE-2 cells,\n                    <jats:italic>casp3<\/jats:italic>\n                    gene expression was increased by MK-801 and OLA treatments alone for 48 h, HAL treatment alone for 24 and 72 h, and co-treatment with MK-801+OLA for 24 and 72 h compared to controls. HAL and OLA suppressed the stimulatory effect of MK-801 on\n                    <jats:italic>casp3<\/jats:italic>\n                    mRNA levels in cells after 48 h of incubation.\n                    <jats:italic>Bax<\/jats:italic>\n                    mRNA levels in mHippoE-2 cells were decreased after HAL treatment for 24 and 48 h, and also after co-treatment with MK-801+HAL for 72 h.\n                    <jats:italic>In vivo<\/jats:italic>\n                    , MK-801 decreased mRNA levels of both pro-apoptotic markers,\n                    <jats:italic>casp3<\/jats:italic>\n                    and\n                    <jats:italic>Bax<\/jats:italic>\n                    , in hippocampus of VEH-treated rats and\n                    <jats:italic>Bax<\/jats:italic>\n                    mRNA levels in hippocampus of HAL-treated animals. OLA reversed the inhibitory effect of MK-801 on\n                    <jats:italic>casp3<\/jats:italic>\n                    expression in the VEH-treated animals. Neither MK-801 nor antipsychotics induced changes in the gene expression of anti-apoptotic marker\n                    <jats:italic>Bcl-2<\/jats:italic>\n                    in mHippoE-2 cells as well as hippocampus of rats.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions.<\/jats:bold>\n                    The results of the present study demonstrate that antipsychotics, HAL and OLA, may affect mRNA levels of pro-apoptotic markers in hippocampal cells\n                    <jats:italic>in vitro<\/jats:italic>\n                    , but not\n                    <jats:italic>in vivo<\/jats:italic>\n                    . The obtained data do not clearly support the assumed potentiating role of MK-801 in inducing apoptosis in specific brain areas and a possible protective role of antipsychotics against induction of apoptosis. The obtained data may contribute to a deeper insight into the neurodevelopmental changes connected with schizophrenia.\n                  <\/jats:p>","DOI":"10.2478\/enr-2023-0019","type":"journal-article","created":{"date-parts":[[2023,8,10]],"date-time":"2023-08-10T12:38:26Z","timestamp":1691671106000},"page":"152-161","source":"Crossref","is-referenced-by-count":6,"title":["Effects of antipsychotics, haloperidol and olanzapine, on the expression of apoptosis-related genes in mouse mHippoE-2 cells and rat hippocampus"],"prefix":"10.2478","volume":"57","author":[{"given":"Jana","family":"Osacka","sequence":"first","affiliation":[{"name":"Institute of Experimental Endocrinology, Biomedical Research Center, Slovak Academy of Sciences , Bratislava , Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Kiss","sequence":"additional","affiliation":[{"name":"Institute of Experimental Endocrinology, Biomedical Research Center, Slovak Academy of Sciences , Bratislava , Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zuzana","family":"Bacova","sequence":"additional","affiliation":[{"name":"Institute of Experimental Endocrinology, Biomedical Research Center, Slovak Academy of Sciences , Bratislava , Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrej","family":"Tillinger","sequence":"additional","affiliation":[{"name":"Institute of Experimental Endocrinology, Biomedical Research Center, Slovak Academy of Sciences , Bratislava , Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2023,8,10]]},"reference":[{"key":"2026042723214634739_j_enr-2023-0019_ref_001","doi-asserted-by":"crossref","unstructured":"Abekawa T, Ito K, Nakagawa S, Nakato Y, Koyama T. 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