{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T22:05:10Z","timestamp":1782857110929,"version":"3.54.5"},"reference-count":14,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:bold>Objective.<\/jats:bold>\n                    The study aims to evaluate the severity of endogenous intoxication and characterize morpho-functional liver changes during experimental acute generalized peritonitis (AGP) in diabetic rats.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods.<\/jats:bold>\n                    Fifty-six adult male Wistar rats were used, including 8 controls and 48 males with experimental pathology. Diabetes mellitus was induced by an intraperitoneal (i.p.) injection of streptozotocin (60 mg\/kg). On day 14, AGP was induced by i.p. injection of a 10% filtered fecal suspension. Endogenous intoxication was assessed by measuring hydrophilic and hydrophobic molecular products in the blood. Liver function was evaluated by serum aminotransferase activity, total protein, and protein fractions. Histological analysis of liver tissue was performed using standard hematoxylin-eosin staining.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results.<\/jats:bold>\n                    A progressive increase in endogenous intoxication was observed peaking on day 7. This was marked by a significant elevation in middle molecular weight molecule (MMWM) concentrations at wavelengths of 254 nm and 280 nm by 103.0% (p&lt;0.001) and 340.0% (p&lt;0.001), respectively. The erythrocyte intoxication index (EII) increased by 148.8% (p&lt;0.001) compared to controls. Concurrently, aminotransferase activity increased, while serum total protein and albumin levels decreased. Histologically, inflammatory infiltration and vascular congestion were evident on day 1 progressing to hepatocellular dystrophy and necrosis by day 3. By day 7, signs of hepatic failure were present including disruption of trabecular architecture, hydropic degeneration, intra-cellular cholestasis, and portal tract expansion due to vascular hyperemia.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions.<\/jats:bold>\n                    Experimental acute generalized peritonitis in diabetic rats resulted in a pronounced endogenous intoxication accompanied by progressive morpho-functional liver damage culminating in hepatic insufficiency by day 7.\n                  <\/jats:p>","DOI":"10.2478\/enr-2025-0027","type":"journal-article","created":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T06:00:57Z","timestamp":1765605657000},"page":"236-243","source":"Crossref","is-referenced-by-count":0,"title":["Endogenous intoxication and morpho-functional changes in the liver during experimental acute generalized peritonitis in diabetic rats"],"prefix":"10.2478","volume":"59","author":[{"given":"Bohdana","family":"Verveha","sequence":"first","affiliation":[{"name":"Department of Pathological Physiology , State Non-Profit Enterprise \u00abDanylo Halytsky Lviv National Medical University\u00bb , Lviv , Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Kondro","sequence":"additional","affiliation":[{"name":"Department of Pathological Physiology , State Non-Profit Enterprise \u00abDanylo Halytsky Lviv National Medical University\u00bb , Lviv , Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petro","family":"Bodnar","sequence":"additional","affiliation":[{"name":"L.Ya. Kovalchuk Department of Surgery No.1 with Urology and Minimally Invasive Surgery , I. Horbachevsky Ternopil National Medical University, Ministry of Health of Ukraine , Ternopil , Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2025,12,12]]},"reference":[{"key":"2026063014594757236_j_enr-2025-0027_ref_001","doi-asserted-by":"crossref","unstructured":"Ahmed MME, Wang T, Luo Y, Ye S, Wu Q, Guo Z, Roebuck BD, Sutter TR, Yang JY. Aldo-Keto Reductase-7A protects liver cells and tissues from acetaminophen-induced oxidative stress and hepatotoxicity. Hepatology 54, 1322\u20131332, 2011.","DOI":"10.1002\/hep.24493"},{"key":"2026063014594757236_j_enr-2025-0027_ref_002","unstructured":"Bancroft JD, Gamble M. (Eds.) Theory and practice of histological techniques, 6th Edition. Churchill Livingstone Elsevier, Philadelphia, PA, Elsevier Health Sciences 2008."},{"key":"2026063014594757236_j_enr-2025-0027_ref_003","doi-asserted-by":"crossref","unstructured":"Barbeiro HV, Catanozi S, Barbeiro DF, Lorigados CB, Passarelli M, Soriano FG. Albumin replacement reduces mortality in hypoalbuminemic rats with LPS-induced endotoxemia, 2024. PREPRINT (Version 1) available at Research Square.","DOI":"10.21203\/rs.3.rs-5361860\/v1"},{"key":"2026063014594757236_j_enr-2025-0027_ref_004","unstructured":"Filip SS, Skrypinets YP. Dynamics of endogenic intoxication findings and non-specific resistance in complex treatment of peritonitis by using endolymphatic combine therapy. Sci Bull Uzhhorod Univ Ser Med 1, 56\u201359, 2019."},{"key":"2026063014594757236_j_enr-2025-0027_ref_005","doi-asserted-by":"crossref","unstructured":"Hnativ VV, Plytka OV. Simulation of peritoneal sepsis and its treatment with serum in an experiment: peculiarities of morphological disorders of liver, spleen and kidney tissues. Rep Morphol 29, 38\u201344, 2023.","DOI":"10.31393\/morphology-journal-2023-29(2)-06"},{"key":"2026063014594757236_j_enr-2025-0027_ref_006","unstructured":"Kumar A, Verma S, Gupta R. Endotoxemia and liver failure in secondary peritonitis: clinical correlations and prognostic implications. World J Gastroenterol 26, 1423\u20131435, 2020."},{"key":"2026063014594757236_j_enr-2025-0027_ref_007","unstructured":"Li Y, Chen Z. Hepatic dysfunction in sepsis and peritonitis: role of inflammatory mediators and microcirculatory disturbances. J Hepatol 75, 894\u2013906, 2021."},{"key":"2026063014594757236_j_enr-2025-0027_ref_008","doi-asserted-by":"crossref","unstructured":"Mammadova ET. Endogenous intoxication indicators in advanced peritonitis and their correction. Klin Khir 88, 39\u201344, 2021.","DOI":"10.26779\/2522-1396.2021.1-2.39"},{"key":"2026063014594757236_j_enr-2025-0027_ref_009","doi-asserted-by":"crossref","unstructured":"Ramos-Lobo AM, Buonfiglio DC, Cipolla-Neto J. Streptozotocin-induced diabetes disrupts the body temperature daily rhythm in rats. Diabetol Metab Syndr 7, 39, 2015.","DOI":"10.1186\/s13098-015-0035-2"},{"key":"2026063014594757236_j_enr-2025-0027_ref_010","doi-asserted-by":"crossref","unstructured":"Recknagel P, Gonnert FA, Westermann M, Lambeck S, Lupp A, Rudiger A, Dyson A, Carr\u00e9 JE, Kortgen A, Krafft C, Popp J, Sponholz C, Fuhrmann V, Hilger I, Claus RA, Riedemann NC, Wetzker R, Singer M, Trauner M, Bauer M. Liver dysfunction and phosphatidylinositol-3-kinase signalling in early sepsis: experimental studies in rodent models of peritonitis. PLoS Med 9, e1001338, 2012.","DOI":"10.1371\/journal.pmed.1001338"},{"key":"2026063014594757236_j_enr-2025-0027_ref_011","doi-asserted-by":"crossref","unstructured":"Reitman S, Frankel S. A colorimetric method for determination of serum glutamate oxaloacetate and glutamic pyruvate transaminase. Am J Clin Pathol 28, 56\u201363, 1957.","DOI":"10.1093\/ajcp\/28.1.56"},{"key":"2026063014594757236_j_enr-2025-0027_ref_012","unstructured":"Savytskyi IV, Mukhin OM, Tsypoviaz SV, Merza YM, Zashchuk RG, Znamerovsky SG, Badiuk NS. Oxidative stress and lipid peroxidation in experimental peritonitis. Pharmacologyonline 1, 125\u2013129, 2021."},{"key":"2026063014594757236_j_enr-2025-0027_ref_013","doi-asserted-by":"crossref","unstructured":"Verveha BM, Gutyj BV, Lishchuk SH, Holubiev MI, Mylostyvyi RV. Oxidative modification of proteins and antioxidant status in blood of the rats with experimental acute generalized peritonitis against the background of streptozotocin-induced diabetes. Regul Mech Biosyst 14, 260\u2013265, 2023.","DOI":"10.15421\/022338"},{"key":"2026063014594757236_j_enr-2025-0027_ref_014","unstructured":"Vlizlo VV, Maksymovych IA, Halyas VL, et al. Laboratory diagnostics in veterinary medicine (reference book). Lviv, 92, 2008."}],"container-title":["Endocrine Regulations"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/reference-global.com\/pdf\/10.2478\/enr-2025-0027","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T21:09:17Z","timestamp":1782853757000},"score":1,"resource":{"primary":{"URL":"https:\/\/reference-global.com\/article\/10.2478\/enr-2025-0027"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,1]]},"references-count":14,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12,12]]},"published-print":{"date-parts":[[2025,1,1]]}},"alternative-id":["10.2478\/enr-2025-0027"],"URL":"https:\/\/doi.org\/10.2478\/enr-2025-0027","relation":{},"ISSN":["1336-0329"],"issn-type":[{"value":"1336-0329","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,1]]}}}