{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T11:00:29Z","timestamp":1791284429755,"version":"4.1.0"},"reference-count":30,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2020,1,25]],"date-time":"2020-01-25T00:00:00Z","timestamp":1579910400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,1,25]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Two species of digenetic trematodes of the genus\n                    <jats:italic>Pleurogenoides viz<\/jats:italic>\n                    .,\n                    <jats:italic>P. cyanophlycti<\/jats:italic>\n                    Shinad &amp; Prasadan (2018a) and\n                    <jats:italic>P. euphlycti<\/jats:italic>\n                    Shinad &amp; Prasadan (2018b) have been described from India. Information regarding the molecular data of various species of the genus\n                    <jats:italic>Pleurogenoides<\/jats:italic>\n                    Travassos, 1921 is virtually lacking. This study addresses the application of molecular markers to validate the phylogenetic position of\n                    <jats:italic>P. cyanophlycti<\/jats:italic>\n                    and\n                    <jats:italic>P. euphlycti<\/jats:italic>\n                    . In the present study, two species\n                    <jats:italic>P. cyanophlycti<\/jats:italic>\n                    and\n                    <jats:italic>P. euphlycti<\/jats:italic>\n                    were collected between January 2016 to October 2017, infecting the freshwater frogs inhabiting the Western Ghats, India. In the present study, the two species were identifi ed morphologically and by PCR amplification of the 28S ribosomal RNA gene. Phylogenetic tree results clearly demonstrate that both\n                    <jats:italic>P. cyanophlycti<\/jats:italic>\n                    and\n                    <jats:italic>P. euphlycti<\/jats:italic>\n                    belongs to the family Pleurogenidae Looss, 1899. Based on these results, we presented and discussed the phylogenetic relationships of\n                    <jats:italic>P. cyanophlycti<\/jats:italic>\n                    and\n                    <jats:italic>P. euphlycti<\/jats:italic>\n                    within family Pleurogenidae from India. Phylogenetic analyses showed that\n                    <jats:italic>P. cyanophlycti<\/jats:italic>\n                    and\n                    <jats:italic>P. euphlycti<\/jats:italic>\n                    cluster according to their vertebrate host and revealed an important congruence between the phylogenetic trees of\n                    <jats:italic>Pleurogenoides<\/jats:italic>\n                    and of their vertebrate hosts.\n                    <jats:italic>P. cyanophlycti<\/jats:italic>\n                    and\n                    <jats:italic>P. euphlycti<\/jats:italic>\n                    clearly constitute a separate, sister branch with other species of the genera,\n                    <jats:italic>Pleurogenoides, Pleurogenes<\/jats:italic>\n                    (\n                    <jats:italic>=Candidotrema<\/jats:italic>\n                    )\n                    <jats:italic>, Prosotocus<\/jats:italic>\n                    and\n                    <jats:italic>Brandesia<\/jats:italic>\n                    . The present study firstly provides important information about the molecular study and phylogenetic analysis of\n                    <jats:italic>P. cyanophlycti<\/jats:italic>\n                    and\n                    <jats:italic>P. euphlycti<\/jats:italic>\n                    . This study will also serve as a baseline for\n                    <jats:italic>Pleurogenoides<\/jats:italic>\n                    species identifi cation for further studies.\n                  <\/jats:p>","DOI":"10.2478\/helm-2020-0006","type":"journal-article","created":{"date-parts":[[2020,1,26]],"date-time":"2020-01-26T04:31:19Z","timestamp":1580013079000},"page":"71-77","source":"Crossref","is-referenced-by-count":2,"title":["Phylogenetic position of\n                    <i>Pleurogenoides<\/i>\n                    species (Plagiorchiida: Pleurogenidae) from the duodenum of Indian skipper frog,\n                    <i>Euphlyctis cyanophlyctis<\/i>\n                    (Amphibia: Dicroglossidae) inhabiting the Western Ghats, India"],"prefix":"10.2478","volume":"57","author":[{"given":"A.","family":"Chaudhary","sequence":"first","affiliation":[{"name":"Molecular Taxonomy Laboratory, Department of Zoology, Chaudhary Charan Singh University , Meerut (U.P.) - 250004 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Shinad","sequence":"additional","affiliation":[{"name":"Ecological Parasitology and Tropical Biodiversity Laboratory, Department of Zoology, Kannur University, Mananthavady Campus , Wayanad , Kerala 670645 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. K.","family":"Prasadan","sequence":"additional","affiliation":[{"name":"Ecological Parasitology and Tropical Biodiversity Laboratory, Department of Zoology, Kannur University, Mananthavady Campus , Wayanad , Kerala 670645 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. S.","family":"Singh","sequence":"additional","affiliation":[{"name":"Molecular Taxonomy Laboratory, Department of Zoology, Chaudhary Charan Singh University , Meerut (U.P.) - 250004 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2020,1,25]]},"reference":[{"key":"2026042803504483843_j_helm-2020-0006_ref_001_w2aab2b8c24b1b7b1ab2b2b1Aa","doi-asserted-by":"crossref","unstructured":"Bella, J.A., Gonzalez-Acuna, D., Tkach, V.V. (2018): First record of Gyrabascus (Digenea, Pleurogenidae) from Dromiciops bozinovici D\u2019Elia et al., 2016 (Marsupialia: Microbiotheriidae) in Chile and its phylogenetic relationships. Comp. 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